See the Unseen: Rapid, Multiparametric Single-Particle Analysis of Extracellular Vesicles With Nano-Flow Cytometry [0.03%]
窥见无形：纳米流式快速分析外泌体的单粒子多参数表征技术
Yunyun Hu,Qiujin Wu,Junyan Chen et al.
Yunyun Hu et al.
Extracellular vesicles (EVs) are heterogeneous lipid membrane-bound nanoparticles that carry diverse biomolecular cargos, including proteins, nucleic acids, lipids, and metabolites. Serving as fundamental mediators of intercellular communic...
Philipp T Kaulich,Hartmut Schlüter,Andreas Tholey
Philipp T Kaulich
Brooke J Wanrooy,Jenny L Wilson,Althea R Suthya et al.
Brooke J Wanrooy et al.
Microglia are abundantly distributed throughout the central nervous system (CNS) to play critical roles in neural development and homeostasis, and act as immune sentinels to constantly monitor their surrounding neural environment. Given the...
Advancing Extracellular Vesicle Research: A Review of Systems Biology and Multiomics Perspectives [0.03%]
外泌体研究进展：系统生物学及多组学观点综述
Gloria Kemunto,Samaneh Ghadami,Kristen Dellinger
Gloria Kemunto
Extracellular vesicles (EVs) are membrane-bound vesicles secreted by various cell types into the extracellular space and play a role in intercellular communication. Their molecular cargo varies depending on the cell of origin and its functi...
Comparative Analysis of Proteoform Clean-Up Methods for In-Depth Top-Down Proteomics [0.03%]
深入顶部蛋白质组学的蛋白变体净化方法比较分析
Alyssa A Williams,Kendalyn N Firenza,Amy K Carfagno et al.
Alyssa A Williams et al.
Proteoforms, the endogenous forms of proteins that take into account all sources of variation (genetic as well as co- and post-translational), may be more relevant for understanding complex biological mechanisms, including disease phenotype...
Lyophilization Improved Efficiency in 2-in-1 Metabolite and Protein Extraction for Plant Multi-Omics [0.03%]
冷冻干燥提高了植物多组学中代谢物和蛋白质联合提取的效率
Qijie Guan,Tahmina Akter,Yatendra Singh et al.
Qijie Guan et al.
Recent advancement of integrated omics has enabled a new era characterized by the systematic identification and functional analysis of a large number of genes and molecules in an organism. For seamless integration of multi-omics data, it is...
Aleksander Moldt Haack,Konstantinos Kalogeropoulos
Aleksander Moldt Haack
Proteolytic cleavage is an irreversible post-translational modification (PTM), and dysregulation of protease activity is often a hallmark in disease. Aberrant proteolysis can alter protein abundance or function, disturbing cellular state an...
Reproductive Aging Impacts the Seminal Fluid Proteome in the Yellow Fever Mosquito, Aedes aegypti [0.03%]
生殖老化影响黄热病蚊子(Aedes aegypti)的精液蛋白组学
Sara V Villa-Arias,Jessica O Atehortúa,Steve Dorus et al.
Sara V Villa-Arias et al.
Aedes aegypti mosquitoes transmit numerous pathogens that pose significant risks to human health despite current interventions. Sex-specific molecules essential for reproduction are valuable potential targets for the suppression of mosquito...
OmixLitMiner 2: Guided Literature Mining for Automated Categorization of Marker Candidates in Omics Studies [0.03%]
OmixLitMiner 2：用于自动分类组学研究中的标记候选物的指导式文献挖掘
Antonia Gocke,Bente Siebels,Jelena Navolić et al.
Antonia Gocke et al.
Omics analyses are crucial for understanding molecular mechanisms in biological research. The vast quantity of detected biomolecules presents a significant challenge in identifying potential biomarkers. Traditional methods rely on labor-int...
Easy Proteomics Sample Preparation: Technical Repeatability and Workflow Optimization Across 8 Biological Matrices in a New Core Facility Setting [0.03%]
简便的蛋白质组学样品制备：新核心设施环境下8种生物基质的技术重复性和工作流程优化
Paraskevi Karousi,Maria Voumvouraki,Panagiota Efstathia Nikolaou et al.
Paraskevi Karousi et al.
Bottom-up proteomics relies on efficient and repeatable sample preparation for accurate protein identification and precise quantification. This study evaluates the performance of adapted SPEED (Sample Preparation by Easy Extraction and Dige...