Probing Early α-Synuclein Oligomers: Insights Into Aggregation Pathways of NACore and preNAC Segments Probed by Trapped Ion-Mobility Mass Spectrometry and Fluorescence Spectroscopy [0.03%]
通过捕集离子淌度质谱和荧光光谱研究早期α-突触核蛋白寡聚体：NACore和preNAC片段聚集途径的见解
Agathe Depraz Depland,Stephanie Mikromanolis,Iuliia Stroganova et al.
Agathe Depraz Depland et al.
Misfolding and aggregation of α-Synuclein (α-Syn) play a central role in Parkinson's disease (PD), with oligomeric intermediates implicated as key toxic species. Here, we investigate the aggregation of two α-Syn segments, the NACore (68G...
High-Resolution Ion Mobility as an Alternative to Quadrupole-Based Precursor Isolation for Reducing Chimeric Fragmentation Spectra in Bottom-Up Proteomics [0.03%]
高分辨率离子淌逸作为四极杆前体选择的替代方法减少从上到下蛋白质组学中的伪并联碎片离子谱图
Isabel R Uribe,Liulin Deng,Leonard Rorrer rd et al.
Isabel R Uribe et al.
To assess the potential for high resolution ion mobility (HRIM) as an alternative means of precursor isolation for mass spectrometry fragmentation analysis we performed a meta-analysis of predicted tryptic peptide features from the human pr...
Mass Spectrometry-Based Quantification of Proteins and Post-Translational Modifications in Dried Blood: Longitudinal Sampling of Patients With Sepsis in Tanzania [0.03%]
基于质量测定的蛋白质和翻译后修饰量化：坦桑尼亚败血症患者纵向检测
Matthew W Foster,Timothy J McMahon,James S Ngocho et al.
Matthew W Foster et al.
The proteomic analysis of blood is routine for disease phenotyping and biomarker development. Blood is commonly separated into soluble and cellular fractions. However, this can introduce pre-analytical variability, and analysis of a single ...
Metabolic Profiling of the EmDia Cohort by LC-MS Reveals Empagliflozin-Intake Associated Regulation of 1,5-anhydroglucitol and Urate [0.03%]
基于LC-MS的EmDia队列代谢轮廓分析发现恩格列净摄入与1,5-脱水山梨醇和尿酸调节有关
Fabian Schmitt,Vincent Ten Cate,Zlatka Fischer et al.
Fabian Schmitt et al.
The EmDia trial, designed to study the effects of the sodium glucose cotransporter-2 (SGLT2) inhibitor empagliflozin on cardiovascular comorbidities in type 2 diabetes mellitus (T2DM) patients, has been investigated for short-term metabolic...
Elenbecestat and Compound 89 Potently Inhibit BACE1 but Not BACE2 When Subchronically Dosed in Non-Human Primates [0.03%]
Elenbecestat和化合物89在非人灵长类动物中短期给药时可有效抑制BACE1但不能抑制BACE2
Sarah K Tschirner,Andree Schmidt,Mana Ito et al.
Sarah K Tschirner et al.
The β-secretase BACE1 (β-site amyloid precursor (APP) cleaving enzyme 1) is a major drug target for Alzheimer's disease (AD), as it catalyzes the first step in amyloid β (Aβ) generation, but has additional substrates and functions, in p...
Plasma Extraction by Acetonitrile and Trichloroacetic Acid With Dithiothreitol and Combination for Low Molecular Weight Proteome Analysis [0.03%]
乙腈和三氯醋酸及二硫苏糖醇组合提取血浆用于低分子量蛋白质组分析
Ting Liu,Shenjian Xu,Chengzhuo Chu et al.
Ting Liu et al.
The low molecular weight (LMW) proteome fraction comprises small proteins and endogenous peptide fragments present in peripheral circulation that could serve as potential diagnostic markers; however, the coverage of the mass spectrometry-ba...
Urinary Peptidomic Profiling In Post-Acute Sequelae of SARS-CoV-2 Infection: A Case-Control Study [0.03%]
SARS-CoV-2感染后急性期后状态的尿肽组学特征：一项病例对照研究
Dilara Gülmez,Justyna Siwy,Katharina Kurz et al.
Dilara Gülmez et al.
Post-acute sequelae of severe acute respiratory syndrome coronavirus 2-infection (PASC) is challenging to diagnose and treat, and its molecular pathophysiology remains unclear. Urinary peptidomics can provide valuable information on urine p...
Proteomics of Prostate Cancer Tissue Small Extracellular Vesicles Reveal Alteration of Metabolism [0.03%]
前列腺癌组织小细胞外囊泡蛋白质组揭示了代谢改变
Lijuan Yu,Ting Ding,Roger Olofsson Bagge et al.
Lijuan Yu et al.
Prostate cancer (PCa) is a leading male malignancy worldwide, with metabolic reprogramming being a critical hallmark of its progression. Extracellular vesicles (EVs) derived from tissues directly reflect the tumor microenvironment, offering...
Weijie Cao,Fangli Hu,Yueyang Li et al.
Weijie Cao et al.
The central dogma of molecular biology, traditionally focused on nucleic acids and proteins, has historically overlooked the crucial involvement of carbohydrates (sugars/glycans) in cellular processes. Carbohydrates combine with lipids, pro...
Proteoform Mapping Calls for a Combined Effort From Bottom-Up and Top-Down Proteomics [0.03%]
整合运用经典的自下而上和自上而下的质谱策略进行蛋白质执行体研究
Yuri E M van der Burgt
Yuri E M van der Burgt
Proteomics has demonstrated that each protein consists of various proteoforms that provide an additional layer of biological data next to gene-, transcript-, or protein expression levels. As a result, proteome analyses are increasingly bein...