David W Greening,Alin Rai,Natalie Turner
David W Greening
Single-Cell Nanodroplet Processing Proteomics Pipeline for Analysis of Human-Derived Microglia [0.03%]
人源小胶质细胞分析的单细胞纳滴处理蛋白质组学流程
Ashley N Ives,James M Fulcher,Alex R Bautista et al.
Ashley N Ives et al.
Single-cell omics tools provide unique insights into heterogeneous cell populations and their responses to stimuli. For example, single-cell RNA sequencing has identified several transcriptionally distinct populations of microglia, which ar...
Practical Impact of Imputation and Batch-Effect Correction for Proteomics/Peptidomics Differential-Abundance Analysis [0.03%]
蛋白质组/肽组差异丰度分析中缺失值插补和批次效应校正的实用影响
Charis Gonidaki,Agnieszka Latosinska,Antonia Vlahou et al.
Charis Gonidaki et al.
MS-based proteomics offers powerful opportunities for biomarker discovery; nevertheless, it is associated with technical challenges, including missing values and batch effects. Although imputation and batch-correction methods are well estab...
Deep Proteomic Profiles of the Antarctic Diatom Fragilariopsis Cylindrus Under Varying Iron and Manganese Conditions [0.03%]
铁和锰条件变化下的南极圆筛藻深层蛋白质谱特征分析
Loay J Jabre,Elden Rowland,J Scott P McCain et al.
Loay J Jabre et al.
Fragilariopsis cylindrus is a key diatom in the Southern Ocean, where low iron and manganese availability constrain primary production and biogeochemical activity. The molecular mechanisms used by polar diatoms, including F. cylindrus, to c...
Identifying Subcellular Structure Components in Escherichia Coli by Crosslinking and SEC-MS [0.03%]
利用交联和尺寸排阻质谱法识别大肠杆菌的胞内组分
Rachel A Victor,Austin Lipinski,Paul R Langlais et al.
Rachel A Victor et al.
Cells are comprised of a broad spectrum of structures that compartmentalize biochemical and signaling mechanisms. These structures can be comprised of many biomolecules, but especially lipids, proteins, and nucleic acids. Techniques are lim...
Quantitative Proteomics Reveals the Adaptive Mechanisms of Aeromonas hydrophila Under Cobalt Stress [0.03%]
定量蛋白质组学揭示了霍里蒙菌在钴胁迫下的适应机制
Xiaowei Zhang,Chenghao Shen,Zhen Qiu et al.
Xiaowei Zhang et al.
Cobalt is an essential micronutrient but becomes toxic at elevated concentrations, requiring microorganisms to balance acquisition and detoxification. Aeromonas hydrophila, an opportunistic aquatic pathogen, is often encountered in metal-co...
Aivett Bilbao,Tim Causon
Aivett Bilbao
Proteomic and Lipidomic Profiling of Immune Cell-Derived Subpopulations of Extracellular Vesicles [0.03%]
免疫细胞源外泌体亚群的蛋白质组和脂质组分析
Anna Lischnig,Nasibeh Karimi,Per Larsson et al.
Anna Lischnig et al.
Extracellular vesicles (EVs) are heterogeneous and play important roles in intercellular communication, contributing to physiological and pathological processes. Since few markers currently exist to differentiate subtypes of EVs, this study...
Brownotate, a Comprehensive Solution to Generate Protein Sequence Databases for Any Species [0.03%]
Brownotate：一种全面的生成任何物种蛋白质序列数据库的方法
Adrien Brown,Alexandre Burel,Sarah Cianférani et al.
Adrien Brown et al.
Proteomics is strengthening research in biology and the diversification of the model organisms studied is very promising for fully understanding the complexity of biological principles. However, the lack of protein sequence databases for ma...
Proteomics in Practice: A Case Study Highlighting Tandem Mass Tag-Based MS for Quantitative Profiling of Extracellular Vesicles and Application to Irradiated Fibroblasts [0.03%]
定量分析辐射诱导的成纤维细胞来源的外泌体的蛋白质组学研究
Greg Berumen Sánchez,Purvi Patel,Kristie Lindsey Rose et al.
Greg Berumen Sánchez et al.
Extracellular vesicles (EVs) are critical mediators of intercellular communication, and valuable sources of biomarkers for diagnostic and therapeutic applications. However, the complexity and heterogeneity of EVs present significant challen...