Dynamic Phosphoproteomic Profiling Captured Early Signaling Events for Cold Acclimation in Larval Zebrafish [0.03%]
用于冷驯化的斑马鱼幼虫早期信号事件的动态磷酸蛋白质组学分析
Junjun Yan,Yong Long,Jing Ren et al.
Junjun Yan et al.
The acclimation at mild low temperature can improve the ability of cold tolerance and protect tissues from stress-induced injury in many species, but mechanisms triggering the signal transduction of cold acclimation remain largely unknown. ...
Comparison of In-Gel and SP3 Based Sample Preparation Protocols for LC-MS/MS Based Proteomics [0.03%]
基于LC-MS/MS蛋白质组学的In-Gel和SP3样品制备方案比较
Luisa Schwarzmüller,Dario Frey,Piotr Zadora et al.
Luisa Schwarzmüller et al.
Sensitivity, robustness, and reproducibility of sample preparation are main determinants of data quality in bottom-up mass spectrometry-based proteomics. To this end, in-gel protein clean-up and digestion has been used for decades and is ch...
Proteomic Characterization of AS1411 Reveals ATP6AP1 as a Mediator of Triple-Negative Breast Cancer Progression [0.03%]
AS1411的蛋白质组表征揭示ATP6AP1作为三阴性乳腺癌进展介导者的作用
Ha-Song Bae,Yun-Jeong Seo,Eun-Bi Kim et al.
Ha-Song Bae et al.
Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by high metastatic potential, poor prognosis, and limited effective therapeutic options. In this study, we investigated the molecular mechanisms un...
Mass Spectrometry Proteomics of the Nanoparticle Corona Is Highly Dependent on Sample Preparation Protocol [0.03%]
样品制备规程决定纳米颗粒蛋白冠质谱蛋白质组学结果
Asia Saorin,Alberto Martinez-Serra,Michael Henry et al.
Asia Saorin et al.
It is widely established that the layers of proteins bound to the surface of nanoparticles (NPs), also known as the protein corona, critically influence their behavior in biological environments. Despite numerous proteomics studies performe...
The N-Glycome to Differentiate Mesenchymal Stem Cells Upon Chondrogenic Differentiation, Dedifferentiation, and Senescence [0.03%]
N-糖组在间充质干细胞软骨分化、去分化和衰老过程中的变化规律研究
Houda Montacir,Karina Biskup,Michael Sittinger et al.
Houda Montacir et al.
Mesenchymal stem cells (MSCs) are adult stem cells able to self-renewal or differentiation into different cell types, including chondrocytes. N-Glycans are post-translational modifications of glycoproteins that contribute to vital cell func...
David W Greening,Yanling Cai,Ying Zhang
David W Greening
Extracellular vesicles (EVs) are fundamental intercellular signaling organelles; these lipid-bilayer-enclosed particles are secreted by all cell types and carry a cargo of proteins, lipids, and nucleic acids to facilitate such cell communic...
Reduction in Synaptic Vesicle Protein Abundance but Increased Amounts of Nsg2 and Lpcat1 in Cerebral Cortices Without the Endosomal SNARE Proteins Vti1a and Vti1b [0.03%]
无终末小体SNARE蛋白Vti1a和Vti1b的大脑皮层中突触囊泡蛋白含量减少但Nsg2和Lpcat1含量增加
Julia Gottschalk,Katharina Kotschnew,Julia Hahn et al.
Julia Gottschalk et al.
Absence of the endosomal SNAREs vti1a and vti1b results in perinatal death and severe neuronal phenotypes in mice, while lack of one of these proteins results in minor phenotypes. Proteomic differences were investigated to obtain a deeper i...
Optimization of Microfluidic Geometry for Extracellular Vesicle Capture Using an Automated Parallel Pattern Search [0.03%]
基于自动化平行模式搜索的用于细胞外囊泡捕获的微流控芯片结构优化
Arvin Lim,A J Tyler,Isha Nayyar et al.
Arvin Lim et al.
Microfluidic affinity-based capture of extracellular vesicles (EVs) holds great promise for disease diagnostics and monitoring therapeutic EV production. However, optimizing microfluidic channel geometries for efficient EV capture remains u...
Image Analysis Platform for Comprehensive Quantification of Extracellular Vesicle Morphology [0.03%]
用于全面量化细胞外囊泡形态的图像分析平台
Alexander Spark,Nan Jiang,Benjamin Purnell et al.
Alexander Spark et al.
Extracellular vesicles (EVs) are membrane-encapsulated nanoparticles with key roles in cell biology and growing importance in biomedicine. However, EVs are challenging to characterize because they are highly heterogeneous. Single EV analysi...
Imaging Mass Spectrometry-Based Assessment of ER, PR, and HER2 Protein Expression in Breast Cancer [0.03%]
基于成像质谱的乳腺癌中ER、PR和HER2蛋白表达评估方法
Kiran K Mangalaparthi,Daigo Gunji,Olajide E Olaleye et al.
Kiran K Mangalaparthi et al.
Imaging mass spectrometry has made significant advancements in recent years owing to its ability to study the spatial localization and abundance of proteins, peptides, lipids, glycans, metabolites, and drugs. In this study, we employed an i...