Urinary Peptidomic Signatures Predict Overall and Progression-Free Survival in Bladder Cancer [0.03%]
尿肽谱可预测膀胱癌的总生存率和无病生存期
Melika Ahangar,Harald Mischak,Napoleon Moulavasilis et al.
Melika Ahangar et al.
Clinicopathologic calculators for bladder cancer (BC) provide only moderate prognostic accuracy and do not capture the underlying molecular phenotypes. Herein, we applied capillary electrophoresis-mass spectrometry (CE-MS) to identify progn...
Parallel Analysis of Acidic and Basic Proteoforms in Cell Lysates via Native Cation and Anion Exchange Chromatography-Native Mass Spectrometry [0.03%]
基于原位阳离子和阴离子交换色谱-原位质谱的细胞裂解液中酸性和碱性蛋白型平行分析
Ziran Zhai,Hafsa Zakri,Matteo Damian et al.
Ziran Zhai et al.
Comprehensive characterization of proteoforms in complex biological systems remains a significant challenge. While native ion-exchange chromatography (IEC) hyphenated with native mass spectrometry (nMS) is a powerful tool for resolving prot...
Branched-Chain Amino and Keto Acids Reduce Hepatocyte Lipid Droplet Size and Number via Distinct Proteomic Pathways [0.03%]
支链氨基酸和酮酸通过不同的蛋白质组学途径减少肝细胞脂滴的大小和数量
Jayasimha R Daddam,Mounica Sura,Edward Kolodziej et al.
Jayasimha R Daddam et al.
Branched-chain amino acids (BCAA) and their corresponding keto acids (BCKA) have been associated with changes in hepatic lipid metabolism and the resulting alterations in intracellular triglyceride concentrations. In this study, we utilized...
Plasma Proteomics Across Three Generations of Mass Spectrometry Instruments: Lessons for Biofluid Method Optimisation [0.03%]
三代质谱仪的血浆蛋白质组学研究：生物液体方法优化的经验教训
Jack Edwards,Dylan H Multari,Toby Dite et al.
Jack Edwards et al.
In biomarker discovery phases clinical proteomics provides large-scale identification and quantification of proteins in donor samples. For these studies plasma is the most frequently used biofluid, as it reflects both physiological and path...
Proteomic Profiling of Primary Hippocampal Neurons Reveals Noncanonical GFAP Expression and Metabolic Adaptations in Glia-Free Culture [0.03%]
原代海马神经元蛋白质组分析揭示无星形胶质细胞培养中的非经典GFAP表达及代谢适应性变化
Dominika Drulis-Fajdasz,Kinga Gostomska-Pampuch,Natalia Pudełko-Malik et al.
Dominika Drulis-Fajdasz et al.
Despite their widespread use as a research model, a comprehensive, quantitative proteomic profile of the cultured hippocampal neurons has remained unexplored. Here, we provide the first global proteomic characterization of primary murine hi...
Maria Frantzi,Felix Keller,Agnieszka Latosinska et al.
Maria Frantzi et al.
Organ fibrosis caused by the presence of excessive extracellular matrix (ECM) is related to mortality. Urinary peptide signatures were reported to be predictive of death in SARS-CoV-2 and chronic kidney disease. Such signatures were compose...
Hypoxia Modulates Nanovesicle Phenotype and Angiogenic Function From Human Pluripotent Stem Cells [0.03%]
低氧调节人多能干细胞的纳米囊泡表型和血管生成功能
Jonathan Lozano,Alin Rai,Ren J Phang et al.
Jonathan Lozano et al.
Nanovesicles (NVs)-generated directly from donor cells-have emerged as important mediators of intercellular communication, drug delivery devices, and in therapeutic applications, including regenerative biology. Top-down strategies to enhanc...
SPROUTS_DB: An Implemented Database of Contaminants for Extracellular Vesicle Proteomics Studies [0.03%]
SPROUTS_DB：一个用于外泌体蛋白质组学研究的污染物实现数据库
Maria Gaetana Giovanna Pittalà,Loredana Leggio,Greta Paternò et al.
Maria Gaetana Giovanna Pittalà et al.
Current proteomics techniques allow rapid identification and quantification of proteins within any given biological source. However, LC-MS/MS proteomics is vulnerable to laboratory and sample-associated contaminants. Therefore, accurate ide...
Unraveling the Molecular Pathways of Insulin-Producing Cells Derived From Placenta Multipotent Stem Cells via Multi-Omics Analysis [0.03%]
基于多组学分析的胎盘多能干细胞衍生胰岛素生成细胞分子通路研究
Jie Xu,Xinggui Shen,Yang Gu et al.
Jie Xu et al.
Stem cell-derived insulin-producing cells (Ins-PCs) hold great promise for diabetes treatment. Placenta-derived multipotent stem cells (PMSCs) are considered an ideal source of Ins-PC generation due to their immunomodulatory and differentia...
Multiple Roles of Protamine Kinase SRPK1 and Phosphatase PP1γ in Sperm Development [0.03%]
组蛋白H3激酶SRPK1和精液PP1γ磷酸酶在精子发生中的多种作用
Alberto de la Iglesia,Mélina Blanco,Meritxell Jodar et al.
Alberto de la Iglesia et al.
Protein phosphorylation regulates key events of male germ cell differentiation. In the testis, SRPK1 and PP1γ are involved in protamine phosphorylation and dephosphorylation, respectively, and their deregulation is linked to defective chro...