Multi-Omics Assessment of Primed Tissue-Specific MSCs-sEV to Unveil Their Regenerative Potential [0.03%]
多组学评估预处理的组织特异性间充质干细胞外泌体以揭示其再生潜力
Yashvi Sharma,Rama N Behera,Naina Soni et al.
Yashvi Sharma et al.
Small extracellular vesicles (sEV) play a pivotal role in intercellular communication and hold immense therapeutic potential. In this study, we aimed to characterize sEV derived from bone marrow mesenchymal stromal cells (BM-MSCs) and Whart...
JUMPshiny: A User-Friendly Platform for Comprehensive Analysis and Visualization of Quantitative Proteomics Data [0.03%]
JUMPshiny：一种用于定量蛋白质组数据全面分析和可视化的用户友好型平台
Aijun Zhang,Yingxue Fu,Zuo-Fei Yuan et al.
Aijun Zhang et al.
Mass spectrometry-based quantitative proteomics has revolutionized our understanding of biological processes and unveiled the molecular mechanisms underlying various diseases. The analysis and visualization of quantitative proteomics data r...
Methionine Oxidation Footprinting in Intact Proteins (MOFIP) Using Top-Down Proteomics [0.03%]
基于顶部-down蛋白质组学的蛋氨酸氧化足迹法(MOFIP)
Anju Teresa Sunny,Kellye A Cupp-Sutton,Zhitao Zhao et al.
Anju Teresa Sunny et al.
Mass spectrometry (MS)-based proteomics methods, including protein footprinting methods such as hydrogen-deuterium exchange mass spectrometry (HDX-MS) and hydroxyl radical footprinting (HRF), can give unique insight into protein structure a...
Isolation and Characterization of Extracellular Vesicles From Ascochyta rabiei, a Necrotrophic Fungal Chickpea Pathogen [0.03%]
从引起 chickpea 病害的专性寄生真菌 Ascochyta rabiei 分离和鉴定细胞外囊泡
Matin Ghaheri,Chamindie Punyadeera,Ido Bar et al.
Matin Ghaheri et al.
Extracellular vesicles produced by pathogenic organisms play a role in host recognition and invasion across kingdoms, including fungi interacting with their plant hosts. Little is known about EVs from phytopathogenic fungi compared to human...
Proteomic Changes in Cancer Cell Lines as a Result of Bacterial Infection [0.03%]
细菌感染对癌细胞系蛋白质组变化的影响
Bo Ren,Kenneth Weke,Darryl Hardie et al.
Bo Ren et al.
Bacterial infections have been implicated in shaping the tumor microenvironment (TME), but their effects on cancer cell proteomes remain unexplored. In this study, we analyzed proteomic changes in melanoma (A375) and ovarian cancer (OVCAR3)...
Computational Approaches for Pathway-Centric Analysis of Protein Post-Translational Modifications [0.03%]
基于途径的蛋白质翻译后修饰计算方法研究
Julian Müller,Bernhard Kuster,Matthew The
Julian Müller
Protein function is dynamically modulated by post-translational modifications (PTMs). Many different types of PTMs can nowadays be identified and quantified at a large scale using mass spectrometry. It is well known that many PTMs have an e...
Katharina Hirn,Sofía Fajardo-Callejón,Dominic Winter
Katharina Hirn
Lysosomes constitute the main degradative organelle of most eukaryotic cells and are capable of breaking down a wide spectrum of biomolecules, including proteins, lipids, glycans, and DNA/RNA. They play crucial roles in the regulation of ce...
Expanding the Reach of Membrane Protein-Ligand Interaction Studies Through the Integration of Mass Spectrometry and Membrane Mimetics [0.03%]
通过整合质谱和膜模拟物来扩展跨膜蛋白-配体相互作用研究的范围
Jonathon C Lambos,Ashim Bhattacharya,Mohammed Al-Seragi et al.
Jonathon C Lambos et al.
Mass spectrometry (MS) offers robust, label-free approaches for characterizing ligand-protein interactions through two main strategies: affinity-based and stability-based assays. However, their application to membrane proteins (MPs)-a major...
Anna V Kolesov,Natalie Reitz,Marley Dewey et al.
Anna V Kolesov et al.
Extracellular vesicles (EVs) and particles (EPs) are diverse micro- and nanoparticles that circulate in bodily fluids and can attach to, or be deposited onto, the extracellular matrix (ECM) and other surfaces. To date, the nomenclature and ...
Comparative Proteomic Profiling of Human Cell Lines Using Capillary and Micro-Pillar Array Columns [0.03%]
毛细管和微柱阵列柱分析的人类细胞系比较蛋白质组学剖析法
Christina B Schroeter,Joao A Paulo
Christina B Schroeter
Chromatographic column selection can impact proteomic profiling, yet comparative studies remain limited. Here, we evaluate the performance of a conventional flame-pulled Accucore packed-bed capillary column and a microfabricated pillar arra...