Lysosomal membrane protein composition, acidic pH and sterol content are regulated via a light-dependent pathway in metazoan cells [0.03%]
线虫细胞溶酶体膜蛋白组成、酸性pH值和固醇含量受光依赖途径调节
M G Swetha,V Sriram,K S Krishnan et al.
M G Swetha et al.
In metazoans, lysosomes are characterized by a unique tubular morphology, acidic pH, and specific membrane protein (LAMP) and lipid (cholesterol) composition as well as a soluble protein (hydrolases) composition. Here we show that perturbat...
Endocytosis and endosomes at the crossroads of regulating trafficking of axon outgrowth-modifying receptors [0.03%]
内吞作用和内涵体在调节轴突生长修饰受体运输方面的交叉路口
Bettina Winckler,Chan Choo Yap
Bettina Winckler
In neurons, many receptors must be localized correctly to axons or dendrites for proper function. During development, receptors for nerve growth and guidance are targeted to axons and localized to growth cones where receptor activation by l...
Endosomally stored MHC class II does not contribute to antigen presentation by dendritic cells at inflammatory conditions [0.03%]
炎症条件下树突状细胞内体储存的MHC II类分子不参与抗原呈递
Toine ten Broeke,Guillaume van Niel,Marca H M Wauben et al.
Toine ten Broeke et al.
Major histocompatibility complex (MHC) class II (MHCII) is constitutively expressed by immature dendritic cells (DC), but has a short half-life as a consequence of its transport to and degradation in lysosomes. For its transfer to lysosomes...
Jun Katahira,Yoshihiro Yoneda
Jun Katahira
Small RNAs with lengths of 20-30 nucleotides, such as microRNAs (miRNAs), play important regulatory roles in various cellular processes. In conventional linear processing pathways, precursors of miRNAs are transported out of the nucleus by ...
Catherine Sem Wegner,Lina M W Rodahl,Harald Stenmark
Catherine Sem Wegner
Subunits of the endosomal sorting complex required for transport (ESCRT) were identified as components of a molecular machinery that sorts ubiquitinated membrane proteins into the intraluminal vesicles (ILVs) of multivesicular endosomes (MV...
Aaron W Lifland,Chiara Zurla,Joana Yu et al.
Aaron W Lifland et al.
Transport of messenger RNAs (mRNAs) in the cytoplasm is essential for localization to translation sites and for post-transcriptional regulation. Utilizing single-RNA sensitive probes and real-time fluorescence microscopy, we accurately quan...
Proline-rich regions and motifs in trafficking: from ESCRT interaction to viral exploitation [0.03%]
富含脯氨酸区域和序列在转运中的作用：从ESCRT相互作用到病毒利用
Xuefeng Ren,James H Hurley
Xuefeng Ren
Most membrane-enveloped viruses bud from infected cells by hijacking the host ESCRT machinery. The ESCRTs are recruited to the budding sites by viral proteins that contain short proline (Pro)-rich motifs (PRMs) known as late domains. The la...
Peroxisome fission is associated with reorganization of specific membrane proteins [0.03%]
过氧化物酶体分裂与特定膜蛋白重组相关
Małgorzata N Cepińska,Marten Veenhuis,Ida J van der Klei et al.
Małgorzata N Cepińska et al.
Membrane remodeling is an important aspect in organelle biogenesis. We show that different peroxisome membrane proteins that play a role in organelle biogenesis and proliferation (Pex8, Pex10, Pex14, Pex25 and Pex11) are subject to spatiote...
Regulation of β-catenin nuclear dynamics by GSK-3β involves a LEF-1 positive feedback loop [0.03%]
GSK-3β通过一个LEF-1正反馈环调节β-catenin核内动态变化
Cara Jamieson,Manisha Sharma,Beric R Henderson
Cara Jamieson
Nuclear localization of β-catenin is integral to its role in Wnt signaling and cancer. Cellular stimulation by Wnt or lithium chloride (LiCl) inactivates glycogen synthase kinase-3β (GSK-3β), causing nuclear accumulation of β-catenin an...
Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion [0.03%]
定量蛋白质组学研究表明酵母后高尔基体囊泡中的SRO7蛋白在蛋白质分泌中起区分作用
Annabelle Forsmark,Guendalina Rossi,Ingrid Wadskog et al.
Annabelle Forsmark et al.
We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spec...