Conformational changes and protein stability of the pro-apoptotic protein Bax [0.03%]
促凋亡蛋白Bax的构象变化及其稳定性研究
Stephanie Bleicken,Kornelius Zeth
Stephanie Bleicken
Pro-apoptotic Bax is a soluble and monomeric protein under normal physiological conditions. Upon its activation substantial structural rearrangements occur: The protein inserts into the mitochondrial outer membrane and forms higher molecula...
Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2, UCP3, UCP4, and UCP5 show different patterns in rat and mice tissues [0.03%]
大鼠和小鼠组织中线粒体解偶联蛋白UCP2、UCP3、UCP4和UCP5的转录本绝对水平显示不同的模式
Lukás Alán,Katarína Smolková,Eva Kronusová et al.
Lukás Alán et al.
Existing controversies led us to analyze absolute mRNA levels of mitochondrial uncoupling proteins (UCP1-UCP5). Individual UCP isoform mRNA levels varied by up to four orders of magnitude in rat and mouse tissues. UCP2 mRNA content was rela...
The fully-active and structurally-stable form of the mitochondrial ATP synthase of Polytomella sp. is dimeric [0.03%]
Polytomella线粒体ATP合酶的全活性和结构稳定形式是二聚体
Alexa Villavicencio-Queijeiro,Miriam Vázquez-Acevedo,Araceli Cano-Estrada et al.
Alexa Villavicencio-Queijeiro et al.
Mitochondrial F(1)F(O)-ATP synthase of chlorophycean algae is a stable dimeric complex of 1,600 kDa. It lacks the classic subunits that constitute the peripheral stator-stalk and the orthodox polypeptides involved in the dimerization of the...
Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content [0.03%]
操纵脂肪酸含量的酵母线粒体介导脂质过氧化作用影响电子传递链的机制研究
Christian Cortés-Rojo,Elizabeth Calderón-Cortés,Mónica Clemente-Guerrero et al.
Christian Cortés-Rojo et al.
Lipoperoxidative damage to the respiratory chain proteins may account for disruption in mitochondrial electron transport chain (ETC) function and could lead to an augment in the production of reactive oxygen species (ROS). To test this hypo...
Effects of Gingko Extract (EGb761) on oxidative damage under different conditions of serum supply [0.03%]
不同血清条件下石杉碱甲对氧化损伤的影响研究
Chun Shi,Zhibing Yao,Jie Xu et al.
Chun Shi et al.
Standardized Ginkgo biloba extract EGb761 is known to have multivalent properties such as anti-oxidation and anti-apoptosis. In this study, we determined in rat pheochromocytoma (PC12) cells effects of EGb761 treatment on oxidative damage u...
Cloning and sequencing of V-ATPase subunit d from mung bean and its function in passive proton transport [0.03%]
绿豆V-ATP酶d亚基的克隆、序列分析及其介导的被动质子转运功能研究
Zhuqing Ouyang,Zhuo Li,Xujia Zhang
Zhuqing Ouyang
We have previously shown that vacuolar H+-ATPase subcomplex V(o) from mung bean contains subunit d, however, its sequence and function were unknown. In the present study, we report the cloning and recombinant over expression of subunit d fr...
Purinergic signalling in rat GFSHR-17 granulosa cells: an in vitro model of granulosa cells in maturing follicles [0.03%]
大鼠GFSHR-17颗粒细胞中嘌呤能信号转导：成熟卵泡颗粒细胞的体外模型
Willem Bintig,Judith Baumgart,Wilhelm J Walter et al.
Willem Bintig et al.
Purinergic signalling in rat GFSHR-17 granulosa cells was characterised by Ca(2+)-imaging and perforated patch-clamp. We observed a resting intracellular Ca(2+)-concentration ([Ca(2+)](i)) of 100 nM and a membrane potential of -40 mV. This ...
Caspase-independent apoptosis in Friend's erythroleukemia cells: role of mitochondrial ATP synthesis impairment in relocation of apoptosis-inducing factor and endonuclease G [0.03%]
溶血性链球菌红白血病细胞的 caspase非依赖凋亡：线粒体ATP合酶功能障碍在凋亡诱导因子和内切酶G重新定位中的作用
Marina Comelli,Nadia Genero,Irene Mavelli
Marina Comelli
Mitochondria have emerged as the central components of both caspase-dependent and independent apoptosis signalling pathways through release of different apoptogenic proteins. We previously documented that parental and differentiated Friend'...
Silencing of mitochondrial alternative oxidase gene of Aspergillus fumigatus enhances reactive oxygen species production and killing of the fungus by macrophages [0.03%]
烟曲霉线粒体替代氧化酶基因沉默增强巨噬细胞杀灭真菌所产生活性氧的能力
Taisa Magnani,Frederico Marianetti Soriani,Vicente de Paulo Martins et al.
Taisa Magnani et al.
We previously demonstrated that conidia from Aspergillus fumigatus incubated with menadione and paraquat increases activity and expression of cyanide-insensitive alternative oxidase (AOX). Here, we employed the RNA silencing technique in A....
Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F(1)-ATPase [0.03%]
大肠杆菌ATP合成酶b二聚体与F(1)-ATP酶结合时的构象变化
Tarek M Zaida,Tassilo Hornung,Oleg A Volkov et al.
Tarek M Zaida et al.
Conformational changes within the subunit b-dimer of the E. coli ATP synthase occur upon binding to the F(1) sector. ESR spectra of spin-labeled b at room temperature indicated a pivotal point in the b-structure at residue 62. Spectra of fr...