Paenibacillus barengoltzii: isolation, growth, and characterization of a high dextrinizer α-amylase [0.03%]
巴根藻芽孢杆菌（Paenibacillus barengoltzii）的分离、生长和高液化型α-淀粉酶的性质分析
Alonso R Poma Ticona,Mário da Silva Neto,Janice Lisboa de Marco et al.
Alonso R Poma Ticona et al.
Currently, the shift to a greener economy requires the prospection of new industrial processes to reduce greenhouse-gas emissions. Enzymatic catalysis is considered a green alternative to traditional industrial processes. Among enzymes of i...
Recombinant protein CP01850 adjuvanted with Iridea cordata or Sacorpletis skottibergii lipid extracts protected mice against infection by Corynebacterium pseudotuberculosis [0.03%]
用Iridea cordata或Sacorpletis skottibergii脂质提取物佐剂的重组CP01850蛋白使小鼠对Corynebacterium pseudotuberculosis感染具有抵抗力
Tallyson Nogueira Barbosa,Nicole Ramos Scholl,Mara Thais de Oliveira Silva et al.
Tallyson Nogueira Barbosa et al.
Recently, new immunomodulatory compounds have benn sought as effective adjuvants in vaccine development. In this context, the bioactive substances from macroalgae stand out, as they can satisfactorily activate an immune response against inf...
Chao Tao,Jizhou Meng,Dan Wen et al.
Chao Tao et al.
Purpose: Currently, a robust process using healthy Chinese hamster ovary (CHO) cells, the dominating host for biopharmaceutical protein therapeutics, is crucial for high productivity and successful scaling-up. However, is...
Bacillus alcalophilus RecJ (BaRecJ) can drive the adaptive evolution of S. cerevisiae [0.03%]
耐碱菌极端耐热核酸外切酶RecJ可驱动酿酒酵母的适应性进化
Jixiang Shang,Yanchao Zhang,Zongjun Xu et al.
Jixiang Shang et al.
With the continuous advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, in vivo mutagenesis methods based on perturbation factors are now widely utilized. We identified a RecJ ...
Optimization and characterization of collagenase KU665299 and its application in effective in-vitro clot digestion [0.03%]
一种新型胶原酶KU665299的优化及表征及其在高效体外溶解血栓中的应用研究
Shikha Chauhan,Kriti Kanwar,Deepika Sharma et al.
Shikha Chauhan et al.
Objective: The study employed response surface methodology (RSM) to optimize physicochemical variables for extracellular collagenase production by gram negative bacterial strain Chryseobacterium contaminans KU665299 under...
A single-vector CRISPR/Cas9 system for genome editing and heterologous enzyme secretion in Saccharomyces cerevisiae: a case study on pectate lyase for coffee mucilage removal [0.03%]
单载体CRISPR / Cas9系统用于基因组编辑和异源酶分泌以去除咖啡果胶中的半乳糖醛酸聚糖酶案例研究应用于酵母酿酒酵母Saccharomyces cerevisiae
La Ho Truc Lam,Nguyen Huynh Ha Nhi,Vo Thi Hoang Lan et al.
La Ho Truc Lam et al.
The CRISPR/Cas9 system facilitates precise genome editing in various organisms. In this study, a single-vector CRISPR/Cas9 system was developed for Saccharomyces cerevisiae, employing a type II Cas9 enzyme from Streptococcus pyogenes and a ...
A novel method for generating baculovirus bacmids using EGFP-mediated purification and linearization [0.03%]
一种利用EGFP介导的纯化和线性化的昆虫杆状病毒基因组细菌人工染色体bacmid的新构建方法
Wujie Su,Haoyi Gu,Xiaoxia Zhang et al.
Wujie Su et al.
Baculovirus bacmids have been widely used in over-expression and gene deletion. Traditionally, baculovirus bacmids are developed by inserting an 8.6 kbp bacterial DNA cassette into baculovirus genomes either through homologous recombination...
Sifan Shangguan,Taichang Wang,Di Zhao et al.
Sifan Shangguan et al.
Purpose: High-throughput screening methods for cytochrome P450 enzymes (P450s), such as colorimetric, mass spectrometric, and fluorescence-based assays, often face limitations in throughput, real-time monitoring, and vers...
Oral administration of Bacillus subtilis spores expressing Staphylococcus aureus IsdB induces mucosal immune responses in mice [0.03%]
口服枯草芽胞杆菌表达金黄色葡萄球菌IsdB蛋白孢子诱导小鼠黏膜免疫应答
Nhi N Y Nguyen,Lan Duong,Tam Th Nguyen et al.
Nhi N Y Nguyen et al.
Purpose: Bacillus subtilis spores, known for their durability and well-developed genetic manipulation tools, show promise for oral vaccine delivery. Staphylococcus aureus is a major global health concern due to multidrug ...
High throughput screening and metabolic engineering of Saccharopolyspora spinosa for spinosad production [0.03%]
放线菌芽孢链丝菌素产量的高效筛选及代谢工程研究
Jiaxin Du,Jiale Zhang,Chen Yang et al.
Jiaxin Du et al.
The macrolide antibiotics spinosad, synthesized by Saccharopolyspora spinosa, is a highly effective, environmentally-friendly insecticide. However, due to poor fermentation performance and difficulties in engineering S. spinosa strains, the...