Production and application of α-amylase, native AmyJ33-ABC, from Bacillus siamensis JJC33M in gelatinized potato starch and its industrial potential [0.03%]
来源于Bacillus siamensis JJC33M的α-淀粉酶AmyJ33-ABC在糊化马铃薯淀粉中的生产与应用及其工业潜力
Sarahi Hernández-Heredia,María Guadalupe Aguilar-Uscanga,Cirilo Nolasco-Hipólito et al.
Sarahi Hernández-Heredia et al.
α-Amylases (EC 3.2.1.1) are endoenzymes that hydrolyze α-1,4-glycosidic bonds in starch to produce maltooligosaccharides with broad industrial applications (food, textile, fermentation, biofuels). Most α-amylases act only on gelatinized ...
λ-exonuclease-driven split G-quadruplex hybridization-based DNA walking system for sensitive microRNA quantification [0.03%]
一种基于λ exonuclease驱动的G-四链体杂交DNA行走系统的高灵敏microRNA检测方法
Huijing Li,Miaohua Ruan
Huijing Li
The precise measurement of microRNAs (miRNAs) is essential for diagnosing newborn pneumonia. This paper presents a simple, sensitive and accurate fluorescence-based technique for miRNA identification, utilizing a λ-exonuclease (λ-Exo)-dri...
Propolis enhanced sodium alginate hydrogel enriched with copper doped mesoporous bioactive glass nanoparticles for advanced burn care applications [0.03%]
银杏叶提取物-壳聚糖复合膜的体外和体内降解性能及成骨细胞相容性研究
Aqsa Aizaz,Muhammad Haseeb Nawaz,Muhammad Sameet Ismat et al.
Aqsa Aizaz et al.
Chronic wounds, with prolonged healing time, remain a formidable challenge due to lack of angiogenesis, antimicrobial effect, and biocompatibility of conventional wound dressings. The current study focuses on developing novel copper-doped m...
An effective strategy for soluble bovine enterokinase expression in Escherichia coli [0.03%]
一种有效的表达于埃希氏大肠杆菌中的可溶性牛_ENTK 的策略
Akanksha,Manjul Tripathi,Krishna Jyoti Mukherjee et al.
Akanksha et al.
Bovine enterokinase light chain (bEkL) is a serine protease, widely used for the specific cleavage of affinity tags from various recombinant proteins. However, getting soluble expression in Escherichia coli is a challenging task given the p...
Exploring the properties of Rhizobium sp. HR1101-4 exopolysaccharide: introducing a novel exopolysaccharide in food industry [0.03%]
地衣芽孢杆菌HR1101-4胞外多糖的性质研究：一种应用于食品行业的新型胞外多糖
Umma Ayman Tishun,Farzana Sayed Sraboni,Mst Mamotaz Mohal et al.
Umma Ayman Tishun et al.
Microbial exopolysaccharides (EPSs) exhibit distinct physiological properties, including anti-inflammatory, anti-tumor, and anti-microbial activities that may have a range of industrial, pharmaceutical, and medical uses. This study aimed to...
Lethal endotoxin (ccdB) based counterselection improved the efficiency of sequential gene editing in Escherichia coli [0.03%]
致死性内毒素(ccdB)反向筛选可提高大肠杆菌逐次基因编辑的效率
Shiyao Zou,Weiqi Chen,Ying Cao et al.
Shiyao Zou et al.
The CRISPR/Cas9-based technology has been used for sequential gene editing in E. coli. The plasmids carrying the sgRNA and/or Cas9 genes need to be cured after each round of editing. Curing of these plasmids, particularly the sgRNA plasmid,...
Immobilizing D-lactonohydrolase with 3D-printed hollow filaments to improve the enzyme stability and reusability [0.03%]
利用3D打印空心纤维固定D-乳酸脱水酶以提高酶稳定性及重复使用性
Xuefeng Tu,Sifang Zhou,Can Yang et al.
Xuefeng Tu et al.
D-pantothenate is one of the essential micronutrients required by the organism. Stereoselective D-lactonohydrolase (D-lac) can hydrolyze DL-pantolactone into D-pantoic acid, the precursor of D-pantothenate. In this study, the D-lac gene fro...
Exploring the diversity of produced water bacteria with hydrocarbon-degrading potential using MALDI-TOF MS and multivariate statistical analyses [0.03%]
基于矩阵辅助激光解析离子化飞行时间质谱及多元统计分析探索石油开采产出水中烃降解菌的多样性研究
Maryam Al-Kaabi,Nabil Zouari,Mohammad Yousaf Ashfaq et al.
Maryam Al-Kaabi et al.
The success of bioremediation of produced water relies on the use of hydrocarbon-degrading bacteria. Hence, the selection of highly tolerant endogenous strains from produced water is crucial to designing successful bioremediation. However, ...
Incorporation of transmembrane protein antigens into phospholipid bilayers supported on silica microbeads using membrane fusion with budded virions of recombinant baculovirus [0.03%]
利用杆状病毒芽子体融合法将跨膜蛋白抗原插入硅珠支撑的磷脂双层膜中
Kohei Nakanishi,Seiwa Nishio,Yushi Isozaki et al.
Kohei Nakanishi et al.
For the reconstitution of artificial cell membranes, silica microbeads are often selected as scaffolds to reinforce fragile lipid membranes; these bilayer-covered beads are often called spherical supported lipid bilayer membranes (SS-BLMs)....
Enhancing lipase activity in Aspergillus niger through CRISPR/Cas9-mediated protease gene knockout and fermentation optimization [0.03%]
通过CRISPR/Cas9介导的蛋白酶基因敲除和发酵优化提高黑曲霉脂肪酶活性
Hongmei Nie,Zebin Wang,Zhenkai Lin et al.
Hongmei Nie et al.
The engineered Aspergillus niger strain AnCALB005 was selected as the research strain, which is a high-yield strain of Candida antarctica B lipase constructed in our laboratory. CRISPR/Cas9-mediated gene knockout was employed to construct t...