Electrostatically-driven fast association and perdeuteration allow detection of transferred cross-relaxation for G protein-coupled receptor ligands with equilibrium dissociation constants in the high-to-low nanomolar range [0.03%]
静电驱动快速关联和全去uter允许检测高至低nanomolar范围的G蛋白偶联受体配体转移交叉松弛
Laurent J Catoire,Marjorie Damian,Marc Baaden et al.
Laurent J Catoire et al.
The mechanism of signal transduction mediated by G protein-coupled receptors is a subject of intense research in pharmacological and structural biology. Ligand association to the receptor constitutes a critical event in the activation proce...
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups [0.03%]
利用肽键碳氧单线态弛豫dispersionNMR光谱技术量化蛋白质毫秒时间尺度的交换过程
Alexandar L Hansen,Lewis E Kay
Alexandar L Hansen
A new pulse sequence is presented for the measurement of relaxation dispersion profiles quantifying millisecond time-scale exchange dynamics of side-chain carbonyl groups in uniformly (13)C labeled proteins. The methodology has been tested ...
Chemical shift correlation at high MAS frequencies employing low-power symmetry-based mixing schemes [0.03%]
基于低功率对称性混合策略的高MAS频率化学移位相关
Christian Herbst,Jirada Herbst,Jörg Leppert et al.
Christian Herbst et al.
An approach for conveniently implementing low-power CN ( n ) (ν) and RN ( n ) (ν) symmetry-based band-selective mixing sequences for generating homo- and heteronuclear chemical shift correlation NMR spectra of low γ nuclei in biological ...
Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy [0.03%]
用于NMR光谱的HIV-1gp120糖蛋白富集同位素外域的哺乳动物产生
Mallika Sastry,Ling Xu,Ivelin S Georgiev et al.
Mallika Sastry et al.
NMR spectroscopic characterization of the structure or the dynamics of proteins generally requires the production of samples isotopically enriched in (15)N, (13)C, or (2)H. The bacterial expression systems currently in use to obtain isotopi...
Backbone resonance assignment and order tensor estimation using residual dipolar couplings [0.03%]
利用剩余各向异性耦合进行骨架共振分配及顺序张量估算
Paul Shealy,Yizhou Liu,Mikhail Simin et al.
Paul Shealy et al.
An NMR investigation of proteins with known X-ray structures is of interest in a number of endeavors. Performing these studies through nuclear magnetic resonance (NMR) requires the costly step of resonance assignment. The prevalent assignme...
Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy [0.03%]
利用双同相/反相(DIPAP)光谱法提高蛋白质中一健和二键(15)N,(13)C (α)耦合常数的测定精度
Frank Löhr,Sina Reckel,Susanne Stefer et al.
Frank Löhr et al.
An extension to HN(CO-α/β-N,C(α)-J)-TROSY (Permi and Annila in J Biomol NMR 16:221-227, 2000) is proposed that permits the simultaneous determination of the four coupling constants (1) J (N'(i)Cα(i)), (2) J (HN(i)Cα(i)), (2) J (Cα(i-1...
How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar Cα-C'/H N-N cross-correlated relaxation [0.03%]
肽单元几何结构的一致性如何？关于偶极子Cα-C'/H-N自旋扩散-relaxation实验的高精度核磁共振研究
Beat Vögeli
Beat Vögeli
Highly precise and accurate measurements of very small NMR cross-correlated relaxation rates, namely those between protein H (i) (N) -N(i) and C (i-1) (α) -C(i-1)' dipoles, are demonstrated with an error of 0.03 s(-1) for GB3. Because the ...
Elucidating slow binding kinetics of a protein without observable bound resonances by longitudinal relaxation NMR spectroscopy [0.03%]
利用纵向弛豫NMR光谱阐明没有可观察到的结合共振的蛋白质的缓慢结合动力学
Kenji Sugase
Kenji Sugase
We developed a new method to elucidate the binding kinetics k(on) and k(off), and the dissociation constant K(D) (=k(off)/k(on)), of protein-protein interactions without observable bound resonances of the protein of interest due to high mol...
Adam D Schuyler,Mark W Maciejewski,Haribabu Arthanari et al.
Adam D Schuyler et al.
The full resolution afforded by high-field magnets is rarely realized in the indirect dimensions of multidimensional NMR experiments because of the time cost of uniformly sampling to long evolution times. Emerging methods utilizing nonunifo...
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies [0.03%]
一种系统诱变驱动的策略用于超分子组装体位点解析NMR研究
Carlos Amero,M Asunción Durá,Marjolaine Noirclerc-Savoye et al.
Carlos Amero et al.
Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance assignment of methyl probes in high molecular weigh...