Complete determination of the Pin1 catalytic domain thermodynamic cycle by NMR lineshape analysis [0.03%]
NMR线形分析确定Pin1催化结构域热力学循环的完整动力学参数
Alexander I Greenwood,Monique J Rogals,Soumya De et al.
Alexander I Greenwood et al.
The phosphorylation-specific peptidyl-prolyl isomerase Pin1 catalyzes the isomerization of the peptide bond preceding a proline residue between cis and trans isomers. To best understand the mechanisms of Pin1 regulation, rigorous enzymatic ...
Dissecting electrostatic interactions in Bacillus circulans xylanase through NMR-monitored pH titrations [0.03%]
利用核磁共振监测的pH滴定分析轮枝芽孢杆菌木聚糖酶中的静电作用力
Lawrence P McIntosh,Daigo Naito,Simon J Baturin et al.
Lawrence P McIntosh et al.
NMR-monitored pH titration curves of proteins provide a rich source of structural and electrostatic information. Although relatively straightforward to measure, interpreting pH-dependent chemical shift changes to obtain site-specific acid d...
Kevin H Gardner,Anthony Mittermaier,Frans A A Mulder
Kevin H Gardner
4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins [0.03%]
用于蛋白质中芳香族氨基酸侧链自动化指认的4D APSY-HCBCD实验
Barbara Krähenbühl,Sebastian Hiller,Gerhard Wider
Barbara Krähenbühl
A four-dimensional (4D) APSY (automated projection spectroscopy)-HBCB(CG)CDHD experiment is presented. This 4D experiment correlates aromatic with aliphatic carbon and proton resonances from the same amino acid side chain of proteins in aqu...
Interactions of protein side chains with RNA defined with REDOR solid state NMR [0.03%]
利用REDOR固态NMR定义蛋白质侧链与RNA的相互作用
Wei Huang,Gabriele Varani,Gary P Drobny
Wei Huang
Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. ...
Alternative SAIL-Trp for robust aromatic signal assignment and determination of the χ(2) conformation by intra-residue NOEs [0.03%]
基于亚残基内NOE的稳健芳香族信号指认及其χ(2)构象确定的替代SAIL-Trp方法
Yohei Miyanoiri,Mitsuhiro Takeda,JunGoo Jee et al.
Yohei Miyanoiri et al.
Tryptophan (Trp) residues are frequently found in the hydrophobic cores of proteins, and therefore, their side-chain conformations, especially the precise locations of the bulky indole rings, are critical for determining structures by NMR. ...
High-resolution membrane protein structure by joint calculations with solid-state NMR and X-ray experimental data [0.03%]
利用固态NMR和X射线实验数据联合计算的高分辨率膜蛋白结构
Ming Tang,Lindsay J Sperling,Deborah A Berthold et al.
Ming Tang et al.
X-ray diffraction and nuclear magnetic resonance spectroscopy (NMR) are the staple methods for revealing atomic structures of proteins. Since crystals of biomolecular assemblies and membrane proteins often diffract weakly and such large sys...
Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methyl-methyl nuclear Overhauser enhancement spectroscopy [0.03%]
利用顺磁去相和甲基-甲基NOE谱对甲基标记蛋白质进行自动化序同位素标记及立体异构体指认
Vincenzo Venditti,Nicolas L Fawzi,G Marius Clore
Vincenzo Venditti
Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignm...
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe [0.03%]
基于片段的配体筛选利用顺磁性镧系元素探针的核磁共振策略
Tomohide Saio,Kenji Ogura,Kazumi Shimizu et al.
Tomohide Saio et al.
A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation enh...
The use of 1H-31P GHMBC and covariance NMR to unambiguously determine phosphate ester linkages in complex polysaccharide mixtures [0.03%]
利用~(1)H-(~31)P GHMBC和协方差NMR确定复杂糖混合物中磷酸酯键的连接性
Edward R Zartler,Gary E Martin
Edward R Zartler
Poly- and oligo-saccharides are commonly employed as antigens in many vaccines. These antigens contain phosphoester structural elements that are crucial to the antigenicity, and hence the effectiveness of the vaccine. Nuclear Magnetic Reson...