Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media? [0.03%]
各向异性介质中分子对齐揭示多结构域蛋白质的协同作用机制
Tairan Yuwen,Carol Beth Post,Nikolai R Skrynnikov
Tairan Yuwen
Many proteins have modular design with multiple globular domains connected via flexible linkers. As a simple model of such system, we study a tandem construct consisting of two identical SH3 domains and a variable-length Gly/Ser linker. Whe...
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy [0.03%]
纵向交换：ZZ交换谱中动力学参数定量的一种新策略
Karin Kloiber,Romana Spitzer,Sarina Grutsch et al.
Karin Kloiber et al.
Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and excha...
Visualizing the principal component of ¹H, ¹⁵N-HSQC NMR spectral changes that reflect protein structural or functional properties: application to troponin C [0.03%]
基于蛋白质结构或功能属性的~1H, ~15N-HSQC核磁共振光谱变化的主要成分可视化：肌钙蛋白C为例
Ian M Robertson,Robert F Boyko,Brian D Sykes
Ian M Robertson
Laboratories often repeatedly determine the structure of a given protein under a variety of conditions, mutations, modifications, or in a number of states. This approach can be cumbersome and tedious. Given then a database of structures, id...
A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data [0.03%]
一种刚性的二硫键连接的硝基苯侧链简化了PRE数据的定量分析
Nicolas L Fawzi,Mark R Fleissner,Nicholas J Anthis et al.
Nicolas L Fawzi et al.
The measurement of (1)H transverse paramagnetic relaxation enhancement (PRE) has been used in biomolecular systems to determine long-range distance restraints and to visualize sparsely-populated transient states. The intrinsic flexibility o...
Measurement of (1)H-(15)N and (1)H-(13)C residual dipolar couplings in nucleic acids from TROSY intensities [0.03%]
基于TROSY信号强度测定核酸中的氢-氮和氢-碳残余偶合常数
Jinfa Ying,Jinbu Wang,Alex Grishaev et al.
Jinfa Ying et al.
Analogous to the recently introduced ARTSY method for measurement of one-bond (1)H-(15)N residual dipolar couplings (RDCs) in large perdeuterated proteins, we introduce methods for measurement of base (13)C-(1)H and (15)N-(1)H RDCs in proto...
Siderocalin Q83 exhibits differential slow dynamics upon ligand binding [0.03%]
配体结合后侧向钙黏蛋白Q83表现出不同的缓慢动力学性质
Nicolas Coudevylle,Leonhard Geist,Matthias Hoetzinger et al.
Nicolas Coudevylle et al.
Siderocalin Q83 is a small soluble protein that has the ability to bind two different ligands (enterobactin and arachidonic acid) simultaneously in two distinct binding sites. Here we report that Q83 exhibits an intriguing dynamic behavior....
Active site dynamics in NADH oxidase from Thermus thermophilus studied by NMR spin relaxation [0.03%]
NMR自旋弛豫研究的硫磺热嗜温菌NADH氧化酶活性位点的动力学问题
Teresa Miletti,Patrick J Farber,Anthony Mittermaier
Teresa Miletti
We have characterized the backbone dynamics of NADH oxidase from Thermus thermophilus (NOX) using a recently-developed suite of NMR experiments designed to isolate exchange broadening, together with (15)N R (1), R (1ρ ), and {(1)H}-(15)N s...
The feasibility of parameterizing four-state equilibria using relaxation dispersion measurements [0.03%]
基于弛豫弥散实验参数化四态平衡的可行性研究
Pilong Li,Ilídio R S Martins,Michael K Rosen
Pilong Li
Coupled equilibria play important roles in controlling information flow in biochemical systems, including allosteric molecules and multidomain proteins. In the simplest case, two equilibria are coupled to produce four interconverting states...
The structure and dynamic properties of the complete histidine phosphotransfer domain of the chemotaxis specific histidine autokinase CheA from Thermotoga maritima [0.03%]
来自海洋栖热菌的趋化专一性的组氨酸自动磷酸化酶CheA的完整His含磷结构域的结构及动态性质
Anh Vu,Damon J Hamel,Hongjun Zhou et al.
Anh Vu et al.
The bacterial histidine autokinase CheA contains a histidine phosphotransfer (Hpt) domain that accepts a phosphate from the catalytic domain and donates the phosphate to either target response regulator protein, CheY or CheB. The Hpt domain...
Mathematical treatment of adiabatic fast passage pulses for the computation of nuclear spin relaxation rates in proteins with conformational exchange [0.03%]
用于计算构象交换蛋白质中核自旋弛豫率的绝热快通脉冲的数学处理方法
Renate Auer,Martin Tollinger,Ilya Kuprov et al.
Renate Auer et al.
Although originally designed for broadband inversion and decoupling in NMR spectroscopy, recent methodological developments have introduced adiabatic fast passage (AFP) pulses into the field of protein dynamics. AFP pulses employ a frequenc...