Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers [0.03%]
在磁性定向的脂质双层中对均一15N标记的内在膜蛋白进行连续固态核磁共振谱学鉴定
Kaustubh R Mote,T Gopinath,Nathaniel J Traaseth et al.
Kaustubh R Mote et al.
Oriented solid-state NMR is the most direct methodology to obtain the orientation of membrane proteins with respect to the lipid bilayer. The method consists of measuring (1)H-(15)N dipolar couplings (DC) and (15)N anisotropic chemical shif...
Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks [0.03%]
生物样本库中常见前处理变异对血清代谢谱的影响
Ophélie Fliniaux,Gwenaelle Gaillard,Antoine Lion et al.
Ophélie Fliniaux et al.
A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h ...
A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility [0.03%]
一种简单且可重现的昆虫细胞内选择性同位素标记氨基酸的方法
Alvar D Gossert,Alexandra Hinniger,Sascha Gutmann et al.
Alvar D Gossert et al.
An easy to use and robust approach for amino acid type selective isotope labeling in insect cells is presented. It relies on inexpensive commercial media and can be implemented in laboratories without sophisticated infrastructure. In contra...
Tim J Stevens,Rasmus H Fogh,Wayne Boucher et al.
Tim J Stevens et al.
Solid-state magic-angle-spinning (MAS) NMR of proteins has undergone many rapid methodological developments in recent years, enabling detailed studies of protein structure, function and dynamics. Software development, however, has not kept ...
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra [0.03%]
梯度增强型异核单量子相关光谱中混相伪峰的产生及消除
Frans A A Mulder,Renee Otten,Ruud M Scheek
Frans A A Mulder
Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become...
Simultaneous measurement of ¹H-¹⁵N and methyl ¹Hm-¹³Cm residual dipolar couplings in large proteins [0.03%]
大蛋白质中1H-15N和甲基1Hm-13Cm剩余偶合的同时测量
Xinli Liao,Raquel Godoy-Ruiz,Chenyun Guo et al.
Xinli Liao et al.
A two-dimensional TROSY-based SIM-(13)C(m)-(1)H(m)/(1)H-(15)N NMR experiment for simultaneous measurements of methyl (1) D (CH) and backbone amide (1) D (NH) residual dipolar couplings (RDC) in {U-[(15)N,(2)H]; Ileδ1-[(13)CH(3)]; Leu,Val-[...
Zn-binding AZUL domain of human ubiquitin protein ligase Ube3A [0.03%]
人 ubiquitin蛋白连接酶Ube3A的Zn-结合AZUL结构域
Alexander Lemak,Adelinda Yee,Irina Bezsonova et al.
Alexander Lemak et al.
Ube3A (also referred to as E6AP for E6 Associated Protein) is a E3 ubiquitin-protein ligase implicated in the development of Angelman syndrome by controlling degradation of synaptic protein Arc and oncogenic papilloma virus infection by con...
Topology and immersion depth of an integral membrane protein by paramagnetic rates from dissolved oxygen [0.03%]
利用溶解氧的顺磁速率测定跨膜蛋白在膜中的拓扑结构和浸入深度
M Sameer Al-Abdul-Wahid,Raffaello Verardi,Gianluigi Veglia et al.
M Sameer Al-Abdul-Wahid et al.
In studies of membrane proteins, knowledge of protein topology can provide useful insight into both structure and function. In this work, we present a solution NMR method for the measurement the tilt angle and average immersion depth of alp...
NMR detection and characterization of sialylated glycoproteins and cell surface polysaccharides [0.03%]
用于糖蛋白及细胞表面多糖的唾液酸检测和表征的NMR技术
Adam W Barb,Darón I Freedberg,Marcos D Battistel et al.
Adam W Barb et al.
Few solution NMR pulse sequences exist that are explicitly designed to characterize carbohydrates (glycans). This is despite the essential role carbohydrate motifs play in cell-cell communication, microbial pathogenesis, autoimmune disease ...
Measurement of amide hydrogen exchange rates with the use of radiation damping [0.03%]
利用辐射阻尼测量酰胺氢交换速率
Jing-Song Fan,Jackwee Lim,Binhan Yu et al.
Jing-Song Fan et al.
A simple method for measuring amide hydrogen exchange rates is presented, which is based on the selective inversion of water magnetization with the use of radiation damping. Simulations show that accurate exchange rates can be measured desp...