Fractional deuteration applied to biomolecular solid-state NMR spectroscopy [0.03%]
固体状态下的生物分子核磁共振光谱中的分数氘标记法
Deepak Nand,Abhishek Cukkemane,Stefan Becker et al.
Deepak Nand et al.
Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of complex biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium t...
Asymmetry of 13C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy [0.03%]
手性13C标记的3-磷酸甘油用于RNA的位点特异性标记的核磁共振光谱学研究
Chandar S Thakur,T Kwaku Dayie
Chandar S Thakur
Selective isotopic labeling provides an unparalleled window within which to study the structure and dynamics of RNAs by high resolution NMR spectroscopy. Unlike commonly used carbon sources, the asymmetry of (13)C-labeled pyruvate provides ...
Al NMR: a novel NMR data processing program optimized for sparse sampling [0.03%]
一种新型的核磁共振数据处理程序-AlNMR优化用于稀疏采样
John M Gledhill Jr,A Joshua Wand
John M Gledhill Jr
Sparse sampling in biomolecular multidimensional NMR offers increased acquisition speed and resolution and, if appropriate conditions are met, an increase in sensitivity. Sparse sampling of indirectly detected time domains combined with the...
Jennifer H Tomlinson,Mike P Williamson
Jennifer H Tomlinson
Temperature coefficients have been measured for backbone amide (1)H and (15)N nuclei in the B1 domain of protein G (GB1), using temperatures in the range 283-313 K, and pH values from 2.0 to 9.0. Many nuclei display pH-dependent coefficient...
Asymmetry of (13)C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy [0.03%]
手性(13)C标记的丙酮酸可有效选择性地对RNA进行NMR光谱分析标记
Chandar S Thakur,T Kwaku Dayie
Chandar S Thakur
Selective isotopic labeling provides an unparalleled window within which to study the structure and dynamics of RNAs by high resolution NMR spectroscopy. Unlike commonly used carbon sources, the asymmetry of (13)C-labeled pyruvate provides ...
Maayan Gal,Katherine A Edmonds,Alexander G Milbradt et al.
Maayan Gal et al.
Experiments detecting low gyromagnetic nuclei have recently been proposed to utilize the relatively slow relaxation properties of these nuclei in comparison to (1)H. Here we present a new type of (15)N direct-detection experiment. Like the ...
Uncovering symmetry-breaking vector and reliability order for assigning secondary structures of proteins from atomic NMR chemical shifts in amino acids [0.03%]
基于氨基酸原子NMR化学位移分配蛋白质二级结构的对称性破缺向量及可靠性排序研究方法
Wookyung Yu,Woonghee Lee,Weontae Lee et al.
Wookyung Yu et al.
Unravelling the complex correlation between chemical shifts of (13) C (α), (13) C (β), (13) C', (1) H (α), (15) N, (1) H ( N ) atoms in amino acids of proteins from NMR experiment and local structural environments of amino acids facilita...
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins [0.03%]
用于大蛋白质固体状态MAS NMR谱学的三维氘-碳相关实验以获得高分辨率
Daniela Lalli,Paul Schanda,Anup Chowdhury et al.
Daniela Lalli et al.
Well-resolved (2)H-(13)C correlation spectra, reminiscent of (1)H-(13)C correlations, are obtained for perdeuterated ubiquitin and for perdeuterated outer-membrane protein G (OmpG) from E. coli by exploiting the favorable lifetime of (2)H d...
Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins [0.03%]
利用顺磁弛豫增强提高快速NMR方法灵敏度及其在固有无序蛋白研究中的应用
François-Xavier Theillet,Andres Binolfi,Stamatis Liokatis et al.
François-Xavier Theillet et al.
We report enhanced sensitivity NMR measurements of intrinsically disordered proteins in the presence of paramagnetic relaxation enhancement (PRE) agents such as Ni(2+)-chelated DO2A. In proton-detected (1)H-(15)N SOFAST-HMQC and carbon-dete...
Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition [0.03%]
去氘氨基酸无细胞合成中的氢交换及抑制方法研究
Marco Tonelli,Kiran K Singarapu,Shin-ichi Makino et al.
Marco Tonelli et al.
Perdeuteration, selective deuteration, and stereo array isotope labeling (SAIL) are valuable strategies for NMR studies of larger proteins and membrane proteins. To minimize scrambling of the label, it is best to use cell-free methods to pr...