Simultaneous acquisition of 13Cα-15N and 1H-15N-15N sequential correlations in proteins: application of dual receivers in 3D HNN [0.03%]
利用双接收器在三维HNN中同时获得蛋白质的13Cα-15N和1H-15N-15N序列表现式
Swagata Chakraborty,Subhradip Paul,Ramakrishna V Hosur
Swagata Chakraborty
We describe here, adaptation of the HNN pulse sequence for multiple nuclei detection using two independent receivers by utilizing the detectable (13)C(α) transverse magnetization which was otherwise dephased out in the conventional HNN exp...
High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument [0.03%]
一种高分辨率的变磁场NMR装置在一台商用仪器上实现生物大分子全范围弛豫和结构研究
Alfred G Redfield
Alfred G Redfield
Improvements are described in a shuttling field-cycling device (Redfield in Magn Reson Chem 41:753-768, 2003), designed to allow widespread access to this useful technique by configuring it as a removable module to a commercial 500 MHz NMR ...
VITAL NMR: using chemical shift derived secondary structure information for a limited set of amino acids to assess homology model accuracy [0.03%]
基于有限氨基酸的化学位移衍生二级结构信息评价同源模型精度的VITAL NMR方法
Michael C Brothers,Anna E Nesbitt,Michael J Hallock et al.
Michael C Brothers et al.
Homology modeling is a powerful tool for predicting protein structures, whose success depends on obtaining a reasonable alignment between a given structural template and the protein sequence being analyzed. In order to leverage greater pred...
Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers [0.03%]
利用长程H/C偶极转移对全去uter的蛋白质进行主链共振 assignments
Rasmus Linser
Rasmus Linser
For micro-crystalline proteins, solid-state nuclear magnetic resonance spectroscopy of perdeuterated samples can provide spectra of unprecedented quality. Apart from allowing to detect sparsely introduced protons and thereby increasing the ...
An NMR-based scoring function improves the accuracy of binding pose predictions by docking by two orders of magnitude [0.03%]
基于NMR的打分函数通过对接大幅度提高结合构象预测准确性
Julien Orts,Stefan Bartoschek,Christian Griesinger et al.
Julien Orts et al.
Low-affinity ligands can be efficiently optimized into high-affinity drug leads by structure based drug design when atomic-resolution structural information on the protein/ligand complexes is available. In this work we show that the use of ...
GUARDD: user-friendly MATLAB software for rigorous analysis of CPMG RD NMR data [0.03%]
GUARDD：用于CPMG RD NMR数据严格分析的用户友好型MATLAB软件
Ian R Kleckner,Mark P Foster
Ian R Kleckner
Molecular dynamics are essential for life, and nuclear magnetic resonance (NMR) spectroscopy has been used extensively to characterize these phenomena since the 1950s. For the past 15 years, the Carr-Purcell Meiboom-Gill relaxation dispersi...
Sugar-to-base correlation in nucleic acids with a 5D APSY-HCNCH or two 3D APSY-HCN experiments [0.03%]
具有5D APHSY-HCNCH或两个3D APHSY-HCN实验的核酸中的糖-碱基相关性
Barbara Krähenbühl,Daniela Hofmann,Christophe Maris et al.
Barbara Krähenbühl et al.
A five-dimensional (5D) APSY (automated projection spectroscopy) HCNCH experiment is presented, which allows unambiguous correlation of sugar to base nuclei in nucleic acids. The pulse sequence uses multiple quantum (MQ) evolution which ena...
Assignment strategies for aliphatic protons in the solid-state in randomly protonated proteins [0.03%]
固相中随机氢化的蛋白质脂肪族质子的分配策略
Sam Asami,Bernd Reif
Sam Asami
Biological solid-state nuclear magnetic resonance spectroscopy developed rapidly in the past two decades and emerged as an important tool for structural biology. Resonance assignment is an essential prerequisite for structure determination ...
Conformational analysis by quantitative NOE measurements of the β-proton pairs across individual disulfide bonds in proteins [0.03%]
利用定量核过度效应法测定蛋白质中个别二硫键上β质子对的构象分析
Mitsuhiro Takeda,Tsutomu Terauchi,Masatsune Kainosho
Mitsuhiro Takeda
NOEs between the β-protons of cysteine residues across disulfide bonds in proteins provide direct information on the connectivities and conformations of these important cross-links, which are otherwise difficult to investigate. With conven...
Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR [0.03%]
利用大肠杆菌野生型和转酮醇酶突变株生成位点选择性¹³C/¹⁵N核苷酸用于RNA的高分辨率NMR标记研究
Chandar S Thakur,Yiling Luo,Bin Chen et al.
Chandar S Thakur et al.
Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is lab...