Non-uniform sampling of similar NMR spectra and its application to studies of the interaction between alpha-synuclein and liposomes [0.03%]
不均匀采集相似NMR谱及其在研究α-突触核蛋白与脂osome相互作用中的应用
Alexandra Shchukina,Thomas C Schwarz,Michał Nowakowski et al.
Alexandra Shchukina et al.
The accelerated acquisition of multidimensional NMR spectra using sparse non-uniform sampling (NUS) has been widely adopted in recent years. The key concept in NUS is that a major part of the data is omitted during measurement, and then rec...
A methyl-TROSY based 13C relaxation dispersion NMR experiment for studies of chemical exchange in proteins [0.03%]
一种新的甲基-TROSY实验用于蛋白质中化学交换的研究
Vitali Tugarinov,James L Baber,G Marius Clore
Vitali Tugarinov
A methyl Transverse Relaxation Optimized Spectroscopy (methyl-TROSY) based, multiple quantum (MQ) 13C Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion NMR experiment is described. The experiment is derived from the previously develope...
NMR detection and conformational dependence of two, three, and four-bond isotope shifts due to deuteration of backbone amides [0.03%]
用于主链酰胺去氢位移的两、三和四键同位素位移的核磁共振检测及构象依赖性
Andrei T Alexandrescu,Aurelio J Dregni,Carolyn M Teschke
Andrei T Alexandrescu
NMR isotope shifts occur due to small differences in nuclear shielding when nearby atoms are different isotopes. For molecules dissolved in 1:1 H2O:D2O, the resulting mixture of N-H and N-D isotopes leads to a small splitting of resonances ...
Sparse pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction [0.03%]
一种非规范氨基酸连接的镧系标签获得的稀疏伪接触位移NMR数据可改善膜蛋白结构预测
Kaitlyn V Ledwitch,Georg Künze,Jacob R McKinney et al.
Kaitlyn V Ledwitch et al.
A single experimental method alone often fails to provide the resolution, accuracy, and coverage needed to model integral membrane proteins (IMPs). Integrating computation with experimental data is a powerful approach to supplement missing ...
Assessing the applicability of 19F labeled tryptophan residues to quantify protein dynamics [0.03%]
评估标记氟的色氨酸残基在量化蛋白质动力学中的应用性
Christina Krempl,Remco Sprangers
Christina Krempl
Nuclear magnetic resonance (NMR) spectroscopy is uniquely suited to study the dynamics of biomolecules in solution. Most NMR studies exploit the spins of proton, carbon and nitrogen isotopes, as these atoms are highly abundant in proteins a...
Three segment ligation of a 104 kDa multi-domain protein by SrtA and OaAEP1 [0.03%]
SrtA和OaAEP1介导的104kDa多结构域蛋白质的三片段连接反应
Stephan B Azatian,Marella D Canny,Michael P Latham
Stephan B Azatian
NMR spectroscopy is an excellent tool for studying protein structure and dynamics which provides a deeper understanding of biological function. As the size of the biomolecule of interest increases, it can become advantageous to dilute the n...
Water irradiation devoid pulses enhance the sensitivity of 1H,1H nuclear Overhauser effects [0.03%]
水辐照无脉冲增强氢的核超化效应灵敏度
V S Manu,Cristina Olivieri,Gianluigi Veglia
V S Manu
The nuclear Overhauser effect (NOE) is one of NMR spectroscopy's most important and versatile parameters. NOE is routinely utilized to determine the structures of medium-to-large size biomolecules and characterize protein-protein, protein-R...
Efficiently driving protein-based fragment screening and lead discovery using two-dimensional NMR [0.03%]
利用二维核磁共振有效推动蛋白为基础的片段筛选和先导发现
Chen Peng,Andrew T Namanja,Eva Munoz et al.
Chen Peng et al.
Fragment-based drug discovery (FBDD) and validation of small molecule binders using NMR spectroscopy is an established and widely used method in the early stages of drug discovery. Starting from a library of small compounds, ligand- or prot...
Investigation of lipid/protein interactions in trifluoroethanol-water mixtures proposes the strategy for the refolding of helical transmembrane domains [0.03%]
三氟乙醇-水混合物中脂质/蛋白质相互作用的调查提出了重构螺旋跨膜区的方法
Vladislav V Motov,Erik F Kot,Alexandra V Shabalkina et al.
Vladislav V Motov et al.
Membrane proteins are one of the keystone objects in molecular biology, but their structural studies often require an extensive search for an appropriate membrane-like environment and an efficient refolding protocol for a recombinant protei...
Band-selective universal 90° and 180° rotation pulses covering the aliphatic carbon chemical shift range for triple resonance experiments on 1.2 GHz spectrometers [0.03%]
在1.2GHz光谱仪上覆盖脂肪碳化学位移范围的三重共振实验用带选择性通用90°和180°翻转脉冲
Stella Slad,Wolfgang Bermel,Rainer Kümmerle et al.
Stella Slad et al.
Biomolecular NMR spectroscopy requires large magnetic field strengths for high spectral resolution. Today's highest fields comprise proton Larmor frequencies of 1.2 GHz and even larger field strengths are to be expected in the future. In pr...