Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag [0.03%]
手性连接基团对刚性的顺磁标记物的立体专一性效应
Jia-Liang Chen,Bin Li,Bo Ma et al.
Jia-Liang Chen et al.
Flexibility between the paramagnetic tag and its protein conjugates is a common yet unresolved issue in the applications of paramagnetic NMR spectroscopy in biological systems. The flexibility greatly attenuates the magnetic anisotropy and ...
Metabolic15N labeling of the N-glycosylated immunoglobulin G1 Fc with an engineered Saccharomyces cerevisiae strain [0.03%]
工程酵母菌株合成N-糖基化抗体IgG1 Fc的全相对原子质量氮同位素标记技术研究
Anjali Shenoy,Alexander R Davis,Elijah T Roberts et al.
Anjali Shenoy et al.
The predominant protein expression host for NMR spectroscopy is Escherichia coli, however, it does not synthesize appropriate post-translation modifications required for mammalian protein function and is not ideal for expressing naturally s...
The time-zero HSQC method improves the linear free energy relationship of a polypeptide chain through the accurate measurement of residue-specific equilibrium constants [0.03%]
时间零点HSQC法通过精确测量特定残基平衡常数改善多肽链的线性自由能关系
Seiichiro Hayashi,Daisuke Kohda
Seiichiro Hayashi
EXSY (exchange spectroscopy) NMR provides the residue-specific equilibrium constants, K, and residue-specific kinetic rate constants, k, of a polypeptide chain in a two-state exchange in the slow exchange regime. A linear free energy relati...
Towards autonomous analysis of chemical exchange saturation transfer experiments using deep neural networks [0.03%]
基于深度神经网络的化学交换饱和转移实验自分析方法研究
Gogulan Karunanithy,Tairan Yuwen,Lewis E Kay et al.
Gogulan Karunanithy et al.
Macromolecules often exchange between functional states on timescales that can be accessed with NMR spectroscopy and many NMR tools have been developed to characterise the kinetics and thermodynamics of the exchange processes, as well as th...
Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins [0.03%]
配体诱导的结构转变结合顺磁离子有助于大蛋白质中甲基集团的NMR鉴定
Lars Mühlberg,Tuncay Alarcin,Thorben Maass et al.
Lars Mühlberg et al.
NMR spectroscopy allows the study of biomolecules in close-to-native conditions. Structural information can be inferred from the NMR spectra when an assignment is available. Protein assignment is usually a time-consuming task, being special...
Fundamental and practical aspects of machine learning for the peak picking of biomolecular NMR spectra [0.03%]
机器学习在生物大分子NMR谱峰识别中的理论与实际问题探讨
Da-Wei Li,Alexandar L Hansen,Lei Bruschweiler-Li et al.
Da-Wei Li et al.
Rapid progress in machine learning offers new opportunities for the automated analysis of multidimensional NMR spectra ranging from protein NMR to metabolomics applications. Most recently, it has been demonstrated how deep neural networks (...
77Se-13C based dipolar correlation experiments to map selenium sites in microcrystalline proteins [0.03%]
利用77Se-13C构建偶极相关实验在微晶蛋白质中寻找硒位置
Caitlin M Quinn,Shiping Xu,Guangjin Hou et al.
Caitlin M Quinn et al.
Sulfur-containing sites in proteins are of great importance for both protein structure and function, including enzymatic catalysis, signaling pathways, and recognition of ligands and protein partners. Selenium-77 is an NMR active spin-1/2 n...
Art Palmer
Art Palmer
Optimization and validation of multi-state NMR protein structures using structural correlations [0.03%]
基于结构相关性多构象NMR蛋白质结构的优化与验证
Dzmitry Ashkinadze,Harindranath Kadavath,Roland Riek et al.
Dzmitry Ashkinadze et al.
Recent advances in the field of protein structure determination using liquid-state NMR enable the elucidation of multi-state protein conformations that can provide insight into correlated and non-correlated protein dynamics at atomic resolu...
Site-specific protein methyl deuterium quadrupolar patterns by proton-detected 3D 2H-13C-1H MAS NMR spectroscopy [0.03%]
位点特异性蛋白质甲基氘四极图案的质子检测三维2H-13C-1H固体魔角旋转核磁共振光谱学研究
Ümit Akbey
Ümit Akbey
Determination of protein structure and dynamics is key to understand the mechanism of protein action. Perdeuterated proteins have been used to obtain high resolution/sensitivty NMR experiments via proton-detection. These methods utilizes 1H...