Conformational distributions of denatured and unstructured proteins are similar to those of 20 × 20 blocked dipeptides [0.03%]
变性蛋白和无规折叠蛋白的构象分布与20×20blocked二肽类似
Kwang-Im Oh,Young-Sang Jung,Geum-Sook Hwang et al.
Kwang-Im Oh et al.
Understanding intrinsic conformational preferences of amino-acids in unfolded proteins is important for elucidating the underlying principles of their stability and re-folding on biological timescales. Here, to investigate the neighbor inte...
High-yield Escherichia coli-based cell-free expression of human proteins [0.03%]
基于大肠杆菌的高产细胞free体系表达人源蛋白
Erich Michel,Kurt Wüthrich
Erich Michel
Production of sufficient amounts of human proteins is a frequent bottleneck in structural biology. Here we describe an Escherichia coli-based cell-free system which yields mg-quantities of human proteins in N-terminal fusion constructs with...
Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility [0.03%]
用于NMR结构研究的蛋白质片段同位素标记：采用蛋白S标签提高表达和溶解度
Hiroshi Kobayashi,G V T Swapna,Kuen-Phon Wu et al.
Hiroshi Kobayashi et al.
A common obstacle to NMR studies of proteins is sample preparation. In many cases, proteins targeted for NMR studies are poorly expressed and/or expressed in insoluble forms. Here, we describe a novel approach to overcome these problems. In...
Anders B Nielsen,Kathrin Székely,Julia Gath et al.
Anders B Nielsen et al.
We present a scheme that allows the simultaneous detection of PAR and PAIN correlation spectra in a single two-dimensional experiment. For both spectra, we obtain almost the same signal-to-noise ratio as if a PAR or PAIN spectrum is recorde...
RNA-PAIRS: RNA probabilistic assignment of imino resonance shifts [0.03%]
RNA-PAIRS：RNA酰胺共振位移的概率分配方法
Arash Bahrami,Lawrence J Clos nd,John L Markley et al.
Arash Bahrami et al.
The significant biological role of RNA has further highlighted the need for improving the accuracy, efficiency and the reach of methods for investigating RNA structure and function. Nuclear magnetic resonance (NMR) spectroscopy is vital to ...
Simultaneous single-structure and bundle representation of protein NMR structures in torsion angle space [0.03%]
扭转角空间中的蛋白质NMR结构的单一体系和束族表示同时进行
Daniel Gottstein,Donata K Kirchner,Peter Güntert
Daniel Gottstein
A method is introduced to represent an ensemble of conformers of a protein by a single structure in torsion angle space that lies closest to the averaged Cartesian coordinates while maintaining perfect covalent geometry and on average equal...
NMR analysis of Lys63-linked polyubiquitin recognition by the tandem ubiquitin-interacting motifs of Rap80 [0.03%]
Rap80的串联泛素相互作用基序识别Lys63连接多聚泛素链的核磁共振分析
Naotaka Sekiyama,Jungoo Jee,Shin Isogai et al.
Naotaka Sekiyama et al.
Ubiquitin is a post-translational modifier that is involved in cellular functions through its covalent attachment to target proteins. Ubiquitin can also be conjugated to itself at seven lysine residues and at its amino terminus to form eigh...
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins [0.03%]
用于固有无序蛋白主链共振.assign的高维和高分辨率脉冲序列
Anna Zawadzka-Kazimierczuk,Wiktor Koźmiński,Hana Sanderová et al.
Anna Zawadzka-Kazimierczuk et al.
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly d...
Recombinant A22(G)-B31 (R)-human insulin. A22 addition introduces conformational mobility in B chain C-terminus [0.03%]
重组A22(G)-B31(R)人胰岛素。A22的添加使β链C端具有构象灵活性
Piotr Borowicz,Elżbieta Bednarek,Wojciech Bocian et al.
Piotr Borowicz et al.
An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media [0.03%]
一种在全去 deuteration 重组蛋白中有效掺入非天然氨基酸的葡萄糖培养基协议
Vincenzo Venditti,Nicolas L Fawzi,G Marius Clore
Vincenzo Venditti
The in vivo incorporation of unnatural amino acids into proteins is a well-established technique requiring an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is incorporated at a position encoded by...