A 2D ¹³C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding [0.03%]
一种使用甲基探针研究缓慢交换蛋白质体系的二维13C-CEST实验及其在蛋白质折叠方面的应用研究
Guillaume Bouvignies,Lewis E Kay
Guillaume Bouvignies
A 2D (13)C Chemical Exchange Saturation Transfer (CEST) experiment is presented for studying slowly exchanging protein systems using methyl groups as probes. The utility of the method is first established through studies of protein L, a sma...
Measurement of ¹⁵N relaxation rates in perdeuterated proteins by TROSY-based methods [0.03%]
利用TROSY方法测量全氘交换蛋白质中的15N弛豫率
Nils-Alexander Lakomek,Jinfa Ying,Ad Bax
Nils-Alexander Lakomek
While extracting dynamics parameters from backbone (15)N relaxation measurements in proteins has become routine over the past two decades, it is increasingly recognized that accurate quantitative analysis can remain limited by the potential...
Wolfgang Bermel,Ivano Bertini,Isabella C Felli et al.
Wolfgang Bermel et al.
The characterization of intrinsically disordered proteins (IDPs) by NMR spectroscopy is made difficult by the extensive spectral overlaps. To overcome the intrinsic low-resolution of the spectra the introduction of high-dimensionality exper...
Resolution-by-proxy: a simple measure for assessing and comparing the overall quality of NMR protein structures [0.03%]
代理决议：一种评估和比较蛋白质NMR结构整体质量的简单措施
Mark Berjanskii,Jianjun Zhou,Yongjie Liang et al.
Mark Berjanskii et al.
In protein X-ray crystallography, resolution is often used as a good indicator of structural quality. Diffraction resolution of protein crystals correlates well with the number of X-ray observables that are used in structure generation and,...
Ivano Bertini,Lucio Ferella,Claudio Luchinat et al.
Ivano Bertini et al.
The MaxOcc web portal is presented for the characterization of the conformational heterogeneity of two-domain proteins, through the calculation of the Maximum Occurrence that each protein conformation can have in agreement with experimental...
A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR [0.03%]
利用双量子固态NMR测定视紫红质第一个光中间体中较大的几何构象变化
Maria Concistrè,Ole G Johannessen,Neville McLean et al.
Maria Concistrè et al.
Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-(13)C(2)-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The ...
Evgenii L Kovrigin
Evgenii L Kovrigin
Biological function of proteins relies on conformational transitions and binding of specific ligands. Protein-ligand interactions are thermodynamically and kinetically coupled to conformational changes in protein structures as conceptualize...
NMR structure note: the ferrous iron transport protein C (FeoC) from Klebsiella pneumoniae [0.03%]
核磁共振结构注释：肺炎克雷伯菌中的铁离子运输蛋白C(FeoC)
Kuo-Wei Hung,Tzu-Hsuan Juan,Yen-Lan Hsu et al.
Kuo-Wei Hung et al.
Georg Kontaxis
Georg Kontaxis
A method for generating protein backbone models from backbone only NMR data is presented, which is based on molecular fragment replacement (MFR). In a first step, the PDB database is mined for homologous peptide fragments using experimental...
4D non-uniformly sampled HCBCACON and ¹J(NCα)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins [0.03%]
用于固有无序蛋白序列鉴定及化学位移分析的4D非均匀采样HCBCACON和15NH(CA)选择性HCBCANCO实验
Jiří Nováček,Noam Y Haba,Jordan H Chill et al.
Jiří Nováček et al.
A pair of 4D NMR experiments for the backbone assignment of disordered proteins is presented. The experiments exploit (13)C direct detection and non-uniform sampling of the indirectly detected dimensions, and provide correlations of the ali...