Eriks Kupče,Lewis E Kay
Eriks Kupče
We introduce the use of multiple receivers applied in parallel for simultaneously recording multi-dimensional data sets of proteins in a single experiment. The utility of the approach is established through the introduction of the 2D (15)N,...
NMR structure note: N-terminal domain of Thermus thermophilus CdnL [0.03%]
NMR结构注释：Thermus thermophilus的CdnL N末端结构域
Aranzazu Gallego-García,Yasmina Mirassou,Montserrat Elías-Arnanz et al.
Aranzazu Gallego-García et al.
High resolution methyl selective ¹³C-NMR of proteins in solution and solid state [0.03%]
溶液和固态中蛋白质高分辨率甲基选择性碳-13核磁共振研究
Garima Jaipuria,Nitin Prakash Lobo,Divya Shet et al.
Garima Jaipuria et al.
New ¹³C-detected NMR experiments have been devised for molecules in solution and solid state, which provide chemical shift correlations of methyl groups with high resolution, selectivity and sensitivity. The experiments achieve selective ...
¹³C relaxation experiments for aromatic side chains employing longitudinal- and transverse-relaxation optimized NMR spectroscopy [0.03%]
利用纵向和横向弛豫最佳化核磁共振光譜法研究芳香側鏈的¹³C弛豫現象
Ulrich Weininger,Carl Diehl,Mikael Akke
Ulrich Weininger
Aromatic side chains are prevalent in protein binding sites, perform functional roles in enzymatic catalysis, and form an integral part of the hydrophobic core of proteins. Thus, it is of great interest to probe the conformational dynamics ...
The J-UNIO protocol for automated protein structure determination by NMR in solution [0.03%]
溶液中利用NMR自动化测定蛋白质结构的J-UNIO协议
Pedro Serrano,Bill Pedrini,Biswaranjan Mohanty et al.
Pedro Serrano et al.
The J-UNIO (JCSG protocol using the software UNIO) procedure for automated protein structure determination by NMR in solution is introduced. In the present implementation, J-UNIO makes use of APSY-NMR spectroscopy, 3D heteronuclear-resolved...
Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing [0.03%]
利用表达蛋白连接和蛋白质反式剪接对来源于大肠杆菌的140千道尔顿双体多域蛋白CheA进行片段标记
Yuichi Minato,Takumi Ueda,Asako Machiyama et al.
Yuichi Minato et al.
Segmental isotopic labeling is a powerful labeling tool to facilitate NMR studies of larger proteins by not only alleviating the signal overlap problem but also retaining features of uniform isotopic labeling. Although two approaches, expre...
Determination of structural fluctuations of proteins from structure-based calculations of residual dipolar couplings [0.03%]
基于结构的残基各向异性偶极耦合计算用于确定蛋白质结构波动
Rinaldo W Montalvao,Alfonso De Simone,Michele Vendruscolo
Rinaldo W Montalvao
Residual dipolar couplings (RDCs) have the potential of providing detailed information about the conformational fluctuations of proteins. It is very challenging, however, to extract such information because of the complex relationship betwe...
Molecular dynamics re-refinement of two different small RNA loop structures using the original NMR data suggest a common structure [0.03%]
利用原始核磁共振数据用分子动力学法对两个小RNA发夹结构的重新优化表明二者可能具有相同结构
Niel M Henriksen,Darrell R Davis,Thomas E Cheatham rd
Niel M Henriksen
Restrained molecular dynamics simulations are a robust, though perhaps underused, tool for the end-stage refinement of biomolecular structures. We demonstrate their utility-using modern simulation protocols, optimized force fields, and incl...
Denise Steiner,Jane R Allison,Andreas P Eichenberger et al.
Denise Steiner et al.
Structural knowledge about proteins is mainly derived from values of observables, measurable in NMR spectroscopic or X-ray diffraction experiments, i.e. absorbed or scattered intensities, through theoretically derived relationships between ...
An automated system designed for large scale NMR data deposition and annotation: application to over 600 assigned chemical shift data entries to the BioMagResBank from the Riken Structural Genomics/Proteomics Initiative internal database [0.03%]
一个自动化系统的设计用于大规模NMR数据递交和标注：应用于向生物磁共振数据库从Riken结构基因组/蛋白质组计划内部数据库递交600多个指认的化学位移数据条目
Naohiro Kobayashi,Yoko Harano,Naoya Tochio et al.
Naohiro Kobayashi et al.
Biomolecular NMR chemical shift data are key information for the functional analysis of biomolecules and the development of new techniques for NMR studies utilizing chemical shift statistical information. Structural genomics projects are ma...