Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles [0.03%]
利用微线圈NMR监测去垢剂混合物中内在膜蛋白OmpW复性的优化条件
Pawel Stanczak,Qinghai Zhang,Reto Horst et al.
Pawel Stanczak et al.
Optimization of aqueous solutions of the integral membrane protein (IMP) OmpW for NMR structure determination has been monitored with micro-coil NMR, which enables the acquisition of NMR spectra using only micrograms of protein and detergen...
Ivano Bertini,Frank Engelke,Leonardo Gonnelli et al.
Ivano Bertini et al.
We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and t...
Transforming between discrete and continuous angle distribution models: application to protein χ₁ torsions [0.03%]
离散和连续角度分布模型之间的转换：应用于蛋白质χ₁扭转角度分布
Jürgen M Schmidt
Jürgen M Schmidt
Two commonly employed angular-mobility models for describing amino-acid side-chain χ(1) torsion conformation, the staggered-rotamer jump and the normal probability density, are discussed and performance differences in applications to scala...
Conformational exchange of aromatic side chains characterized by L-optimized TROSY-selected ¹³C CPMG relaxation dispersion [0.03%]
L-优化TROSY选择性13C CP-MG弛豫展宽法表征芳香族侧链的构象交换效应
Ulrich Weininger,Michal Respondek,Mikael Akke
Ulrich Weininger
Protein dynamics on the millisecond time scale commonly reflect conformational transitions between distinct functional states. NMR relaxation dispersion experiments have provided important insights into biologically relevant dynamics with s...
Compressed sensing reconstruction of undersampled 3D NOESY spectra: application to large membrane proteins [0.03%]
欠采样三维NOESY谱的压缩感知重建及在大型膜蛋白上的应用
Mark J Bostock,Daniel J Holland,Daniel Nietlispach
Mark J Bostock
Central to structural studies of biomolecules are multidimensional experiments. These are lengthy to record due to the requirement to sample the full Nyquist grid. Time savings can be achieved through undersampling the indirectly-detected d...
Joshua M Ward,Nikolai R Skrynnikov
Joshua M Ward
Main-chain (1)H(N)-(15)N residual dipolar couplings (RDCs) ranging from approximately -200 to 200 Hz have been measured for ubiquitin under strong alignment conditions in Pf1 phage. This represents a ten-fold increase in the degree of align...
Independent alignment of RNA for dynamic studies using residual dipolar couplings [0.03%]
基于残差各向异性耦合的RNA分子独立取向及动态学研究
Michael F Bardaro Jr,Gabriele Varani
Michael F Bardaro Jr
Molecular motion and dynamics play an essential role in the biological function of many RNAs. An important source of information on biomolecular motion can be found in residual dipolar couplings which contain dynamics information over the e...
NMR structure note: solution structure of Ca²⁺ binding domain 2B of the third isoform of the Na⁺/Ca²⁺ exchanger [0.03%]
核磁结构报告：Na+/Ca2+交换器同种异构体3的第二个钙结合域的溶液结构注解
Vincent Breukels,Wouter G Touw,Geerten W Vuister
Vincent Breukels
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra [0.03%]
TSAR：一个使用高维、高分辨率光谱二维切片进行自动共振分配的程序
Anna Zawadzka-Kazimierczuk,Wiktor Koźmiński,Martin Billeter
Anna Zawadzka-Kazimierczuk
While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decr...
Automated protein backbone assignment using the projection-decomposition approach [0.03%]
基于投影-分解法的蛋白质主链自动化归属
Jonas Fredriksson,Wolfgang Bermel,Doroteya K Staykova et al.
Jonas Fredriksson et al.
Spectral projection experiments by NMR in conjunction with decomposition analysis have been previously introduced for the backbone assignment of proteins; various pulse sequences as well as the behaviour with low signal-to-noise or chemical...