The protein amide ¹H(N) chemical shift temperature coefficient reflects thermal expansion of the N-H···O=C hydrogen bond [0.03%]
蛋白质酰胺氢核磁共振化学位移的温度系数反映了N-H···O=C氢键的热膨胀效应
Jingbo Hong,Qingqing Jing,Lishan Yao
Jingbo Hong
The protein amide (1)H(N) chemical shift temperature coefficient can be determined with high accuracy by recording spectra at different temperatures, but the physical mechanism responsible for this temperature dependence is not well underst...
Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic ¹⁵N chemical shielding anisotropy tensors [0.03%]
蛋白质中丝氨酸、苏氨酸和酪氨酸O-磷酸化对酰胺₁⁵N化学屏蔽各向异性张量的影响
Jiří Emmer,Andrea Vavrinská,Vladimír Sychrovský et al.
Jiří Emmer et al.
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic (15)N chemical shielding anisotropy (CSA) tensor in the context of the complex chemical environments of prot...
Biosynthetically directed ²H labelling for stereospecific resonance assignments of glycine methylene groups [0.03%]
以生物合成为导向的同位素标记技术在甘氨酸亚甲基立体位共振分配中的应用
Karin V Loscha,Gottfried Otting
Karin V Loscha
Stereospecific resonance assignments of the α-protons of glycine are often difficult to obtain by measurements of scalar coupling constants or nuclear Overhauser effects. Here we show that these stereospecific resonance assignments can rea...
GFT projection NMR for efficient (1)H/ (13)C sugar spin system identification in nucleic acids [0.03%]
高效区分核酸中(1)H/(13)C糖自旋体系的GFT Projection NMR法
Hanudatta S Atreya,Bharathwaj Sathyamoorthy,Garima Jaipuria et al.
Hanudatta S Atreya et al.
A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify (1)H/(13)C sugar spin systems in (13)C labeled nucleic acids. This experiment enables rapid ...
Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements [0.03%]
利用双电子-电子共振及顺磁弛豫增强法测定同二聚体亚基交换速率常数
Yunhuang Yang,Theresa A Ramelot,Shuisong Ni et al.
Yunhuang Yang et al.
Here, we report novel methods to measure rate constants for homodimer subunit exchange using double electron-electron resonance (DEER) electron paramagnetic resonance spectroscopy measurements and nuclear magnetic resonance spectroscopy bas...
Database proton NMR chemical shifts for RNA signal assignment and validation [0.03%]
用于RNA信号指认与验证的数据库质子NMR化学位移值
Shawn Barton,Xiao Heng,Bruce A Johnson et al.
Shawn Barton et al.
The Biological Magnetic Resonance Data Bank contains NMR chemical shift depositions for 132 RNAs and RNA-containing complexes. We have analyzed the (1)H NMR chemical shifts reported for non-exchangeable protons of residues that reside withi...
Solid state NMR of proteins at high MAS frequencies: symmetry-based mixing and simultaneous acquisition of chemical shift correlation spectra [0.03%]
固体核磁中的蛋白质高转速魔角旋转技术：基于对称性的混合及化学位移相关光谱的同时获取
Peter Bellstedt,Christian Herbst,Sabine Häfner et al.
Peter Bellstedt et al.
We have carried out chemical shift correlation experiments with symmetry-based mixing sequences at high MAS frequencies and examined different strategies to simultaneously acquire 3D correlation spectra that are commonly required in the str...
Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies [0.03%]
利用核磁共振和光学光谱技术对海洋原生蛋白紫红质地面态动力学的表征研究
Jochen Stehle,Frank Scholz,Frank Löhr et al.
Jochen Stehle et al.
We characterized the dynamics of proteorhodopsin (PR), solubilized in diC7PC, a detergent micelle, by liquid-state NMR spectroscopy at T = 323 K. Insights into the dynamics of PR at different time scales could be obtained and dynamic hot sp...
A study on the influence of fast amide exchange on the accuracy of (15)N relaxation rate constants [0.03%]
关于快速酰胺交换对15N弛豫速率常数准确性影响的研究
Simon Jurt,Oliver Zerbe
Simon Jurt
(15)N relaxation rates of amide moieties provide insight both into global as well as local backbone dynamics of peptides and proteins. As the differences in the relaxation rates in general are small, their accurate determination is of prime...
Sabine Gradmann,Christian Ader,Ines Heinrich et al.
Sabine Gradmann et al.
We present a computational environment for Fast Analysis of multidimensional NMR DAta Sets (FANDAS) that allows assembling multidimensional data sets from a variety of input parameters and facilitates comparing and modifying such "in silico...