Paramagnetic NMR to study iron sulfur proteins: 13C detected experiments illuminate the vicinity of the metal center [0.03%]
利用顺磁性NMR研究含铁硫蛋白：13C检测实验揭示金属活性中心附近的环境
Leonardo Querci,Deborah Grifagni,Inês B Trindade et al.
Leonardo Querci et al.
The robustness of NMR coherence transfer in proximity of a paramagnetic center depends on the relaxation properties of the nuclei involved. In the case of Iron-Sulfur Proteins, different pulse schemes or different parameter sets often provi...
Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics [0.03%]
一种用于研究蛋白质侧链相互作用和动态的13C甲基标记异亮氨酸前体的合成
Theresa Höfurthner,Giorgia Toscano,Georg Kontaxis et al.
Theresa Höfurthner et al.
In this study, we present the synthesis and incorporation of a metabolic isoleucine precursor compound for selective methylene labeling. The utility of this novel α-ketoacid isotopologue is shown by incorporation into the protein Brd4-BD1,...
Breaking boundaries: TINTO in POKY for computer vision-based NMR walking strategies [0.03%]
突破界限：在POKY中使用基于计算机视觉的NMR行走策略的TINTO研究
Andrea Estefania Lopez Giraldo,Zowie Werner,Mehdi Rahimi et al.
Andrea Estefania Lopez Giraldo et al.
Nuclear magnetic resonance is a crucial technique for studying biological complexes, as it provides precise structural and dynamic information at the atomic level. However, the process of assigning resonances can be time-consuming and chall...
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins [0.03%]
一种用于生物反应器辅助在细胞内核磁共振测量核酸和蛋白质的热敏凝胶基质
Matej Dzurov,Šárka Pospíšilová,Michaela Krafčíková et al.
Matej Dzurov et al.
Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by prolonging the measurement time available to acquire spectral information about biomacromolecules in metabolically active cells. Bioreactor technolog...
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study [0.03%]
膜蛋白动态特性的共振赋值谱获取策略：以G蛋白偶联受体为例
Evan J van Aalst,Jun Jang,Ty C Halligan et al.
Evan J van Aalst et al.
In protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heter...
Improved spectral resolution of [13C,1H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive 13C-labelled precursors [0.03%]
通过廉价的13C标记底物从细胞游离合成中提高蛋白质中芳香族氨基酸残基[13C,1H] HSQC光谱的光谱分辨率
Damian Van Raad,Thomas Huber,Gottfried Otting
Damian Van Raad
Cell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCe...
E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation [0.03%]
大肠杆菌"Stablelabel" S30裂解物在优化的无细胞NMR样品制备中的应用
Roman Levin,Frank Löhr,Betül Karakoc et al.
Roman Levin et al.
Cell-free (CF) synthesis with highly productive E. coli lysates is a convenient method to produce labeled proteins for NMR studies. Despite reduced metabolic activity in CF lysates, a certain scrambling of supplied isotope labels is still n...
Studying micro to millisecond protein dynamics using simple amide 15N CEST experiments supplemented with major-state R2 and visible peak-position constraints [0.03%]
基于主要状态R2和可见峰位置的简单酰胺15N CEST实验研究微秒至毫秒级蛋白质动态变化
Nihar Pradeep Khandave,Ashok Sekhar,Pramodh Vallurupalli
Nihar Pradeep Khandave
Over the last decade amide 15N CEST experiments have emerged as a popular tool to study protein dynamics that involves exchange between a 'visible' major state and sparsely populated 'invisible' minor states. Although initially introduced t...
Efficient 18.8 T MAS-DNP NMR reveals hidden side chains in amyloid fibrils [0.03%]
高效18.8 T MAS-DNP NMR揭示淀粉样纤维中隐藏的侧链
Alons Lends,Nicolas Birlirakis,Xinyi Cai et al.
Alons Lends et al.
Amyloid fibrils are large and insoluble protein assemblies composed of a rigid core associated with a cross-β arrangement rich in β-sheet structural elements. It has been widely observed in solid-state NMR experiments that semi-rigid prot...
1H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR [0.03%]
利用MAS NMR研究高度灵活的质子化生物分子中的碳-碳网络
Salima Bahri,Adil Safeer,Agnes Adler et al.
Salima Bahri et al.
In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly fl...