Solution structure of the Magnaporthe oryzae avirulence protein AvrPiz-t [0.03%]
稻瘟菌效应蛋白AvrPi-z的溶液结构
Zhi-Min Zhang,Xu Zhang,Zi-Ren Zhou et al.
Zhi-Min Zhang et al.
Efficient resonance assignment of proteins in MAS NMR by simultaneous intra- and inter-residue 3D correlation spectroscopy [0.03%]
利用同时的单残差和双残差三维相关光谱有效进行蛋白质动态核磁共振实验中的精简共振分配
Eugenio Daviso,Matthew T Eddy,Loren B Andreas et al.
Eugenio Daviso et al.
Resonance assignment is the first step in NMR structure determination. For magic angle spinning NMR, this is typically achieved with a set of heteronuclear correlation experiments (NCaCX, NCOCX, CONCa) that utilize SPECIFIC-CP (15)N-(13)C t...
Detection of closed influenza virus hemagglutinin fusion peptide structures in membranes by backbone (13)CO- (15)N rotational-echo double-resonance solid-state NMR [0.03%]
利用固态NMR的方法在膜蛋白中检测血凝素融合肽结构
Ujjayini Ghosh,Li Xie,David P Weliky
Ujjayini Ghosh
The influenza virus fusion peptide is the N-terminal ~20 residues of the HA2 subunit of the hemagglutinin protein and this peptide plays a key role in the fusion of the viral and endosomal membranes during initial infection of a cell. The f...
Lena Buchner,Elena Schmidt,Peter Güntert
Lena Buchner
Peak lists are commonly used in NMR as input data for various software tools such as automatic assignment and structure calculation programs. Inconsistencies of chemical shift referencing among different peak lists or between peak and chemi...
Probing non-specific interactions of Ca²⁺-calmodulin in E. coli lysate [0.03%]
Ca²⁺-钙调素在大肠杆菌裂解物中的非特异性相互作用研究
Michael P Latham,Lewis E Kay
Michael P Latham
The biological environment in which a protein performs its function is a crowded milieu containing millions of molecules that can potentially lead to a great many transient, non-specific interactions. NMR spectroscopy is especially well sui...
Fast hydrogen exchange affects ¹⁵N relaxation measurements in intrinsically disordered proteins [0.03%]
快速氢交换影响内在无序蛋白质中的氮－15弛豫测量
Seho Kim,Kuen-Phon Wu,Jean Baum
Seho Kim
Unprotected amide protons can undergo fast hydrogen exchange (HX) with protons from the solvent. Generally, NMR experiments using the out-and-back coherence transfer with amide proton detection are affected by fast HX and result in reduced ...
Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins [0.03%]
提高固有无序蛋白多维核磁共振谱化学班的分散度
Wolfgang Bermel,Marta Bruix,Isabella C Felli et al.
Wolfgang Bermel et al.
Intrinsically disordered proteins (IDPs) have recently attracted the attention of the scientific community challenging the well accepted structure-function paradigm. In the characterization of the dynamic features of proteins nuclear magnet...
Virginie Gervais,Sébastien Campagne,Jade Durand et al.
Virginie Gervais et al.
The THAP (THanatos-Associated Protein) domain is an evolutionary conserved C2CH zinc-coordinating domain shared with a large family of cellular factors (THAP proteins). Many members of the THAP family act as transcription factors that contr...
James H Prestegard,Sarata C Sahu,Wendy K Nkari et al.
James H Prestegard et al.
While chemical shift prediction has played an important role in aspects of protein NMR that include identification of secondary structure, generation of torsion angle constraints for structure determination, and assignment of resonances in ...
MAP-XSII: an improved program for the automatic assignment of methyl resonances in large proteins [0.03%]
MAP-XSII：一种用于大型蛋白质甲基共振自动指认的改进程序
Yingqi Xu,Stephen Matthews
Yingqi Xu
NMR studies of large proteins have gathered much interest in recent years, especially after methyl-transverse relaxation optimized spectroscopy was successfully applied to systems as large as ~1 MDa in molecular weight. However, to fully ta...