Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex [0.03%]
结合核磁共振和小角X射线散射及中子散射分析三元蛋白-RNA复合物的结构
Janosch Hennig,Iren Wang,Miriam Sonntag et al.
Janosch Hennig et al.
Many processes in the regulation of gene expression and signaling involve the formation of protein complexes involving multi-domain proteins. Individual domains that mediate protein-protein and protein-nucleic acid interactions are typicall...
Nmrglue: an open source Python package for the analysis of multidimensional NMR data [0.03%]
Nmrglue：一个用于分析多维核磁共振数据的开源Python包
Jonathan J Helmus,Christopher P Jaroniec
Jonathan J Helmus
Nmrglue, an open source Python package for working with multidimensional NMR data, is described. When used in combination with other Python scientific libraries, nmrglue provides a highly flexible and robust environment for spectral process...
Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA [0.03%]
应用核磁共振技术解析折叠蛋白质中的磷酸化事件：蛋白激酶A对Pin1脯氨酰-tRNA合成酶结构域的磷酸化作用
Caroline Smet-Nocca,Hélène Launay,Jean-Michel Wieruszeski et al.
Caroline Smet-Nocca et al.
The Pin1 protein plays a critical role in the functional regulation of the hyperphosphorylated neuronal Tau protein in Alzheimer's disease and is by itself regulated by phosphorylation. We have used Nuclear Magnetic Resonance (NMR) spectros...
BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins [0.03%]
用于大无序蛋白质高效共振分配的BEST-TROSY实验
Zsofia Solyom,Melanie Schwarten,Leonhard Geist et al.
Zsofia Solyom et al.
The characterization of the conformational properties of intrinsically disordered proteins (IDPs), and their interaction modes with physiological partners has recently become a major research topic for understanding biological function on t...
Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames [0.03%]
基于多旋转框架的宽带相关NMR谱脉冲序列设计
Paul Coote,Haribabu Arthanari,Tsyr-Yan Yu et al.
Paul Coote et al.
We present a method for designing radio-frequency (RF) pulses for broadband or multi-band isotropic mixing at low power, suitable for protein NMR spectroscopy. These mixing pulses are designed analytically, rather than by numerical optimiza...
Biosynthetic engineered B28(K)-B29(P) human insulin monomer structure in water and in water/acetonitrile solutions [0.03%]
水中及水/乙腈溶液中生物合成工程胰岛素单体B28(K)-B29(P)的人类胰岛素结构
Piotr Borowicz,Wojciech Bocian,Jerzy Sitkowski et al.
Piotr Borowicz et al.
Probing slowly exchanging protein systems via ¹³Cα-CEST: monitoring folding of the Im7 protein [0.03%]
利用13CA-CEST探测缓慢交换的蛋白质体系：监测Im7蛋白折叠过程
Alexandar L Hansen,Guillaume Bouvignies,Lewis E Kay
Alexandar L Hansen
A¹³C(α) chemical exchange saturation transfer based experiment is presented for the study of protein systems undergoing slow interconversion between an 'observable' ground state and one or more 'invisible' excited states. Here a labeling...
Leonardus M I Koharudin,Rolf Boelens,Rob Kaptein et al.
Leonardus M I Koharudin et al.
Structure determination of protein-nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA-nucleic acid complex, guided entirely by results from nuclear magnetic resonances spectr...
Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra [0.03%]
固态MAS核磁共振光谱的优异分辨率来源于酿酒酵母表达的人类膜蛋白aquaporin-1
Sanaz Emami,Ying Fan,Rachel Munro et al.
Sanaz Emami et al.
One of the biggest challenges in solid-state NMR studies of membrane proteins is to obtain a homogeneous natively folded sample giving high spectral resolution sufficient for structural studies. Eukaryotic membrane proteins are especially d...
An R(1ρ) expression for a spin in chemical exchange between two sites with unequal transverse relaxation rates [0.03%]
两交换位点横向弛豫速率不等时化学交换的自旋R(1ρ)表达式研究
Andrew J Baldwin,Lewis E Kay
Andrew J Baldwin
An analytical expression is derived for the rotating frame relaxation rate, R(1ρ), of a spin exchanging between two sites with different transverse relaxation times. A number of limiting cases are examined, with the equation reducing to fo...