Determining the depth of insertion of dynamically invisible membrane peptides by gel-phase ¹H spin diffusion heteronuclear correlation NMR [0.03%]
利用凝胶相¹H自旋扩散异核相关NMR确定动态不可见膜肽的插入深度
T Wang,H Yao,M Hong
T Wang
Solid-state NMR determination of the depth of insertion of membrane peptides and proteins has so far utilized (1)H spin diffusion and paramagnetic relaxation enhancement experiments, which are typically conducted in the liquid-crystalline p...
Can metal ion complexes be used as polarizing agents for solution DNP? A theoretical discussion [0.03%]
金属离子配合物能否作为溶液DNP的极化剂？理论探讨
Claudio Luchinat,Giacomo Parigi,Enrico Ravera
Claudio Luchinat
Dynamic nuclear polarization (DNP) can be used to dramatically increase the NMR signal intensities in solutions and solids. DNP is usually performed using nitroxide radicals as polarizing agents, characterized by sharp EPR lines, fast rotat...
Frederik Hefke,Roland Schmucki,Peter Güntert
Frederik Hefke
Peak overlap is one of the major factors complicating the analysis of biomolecular NMR spectra. We present a general method for predicting the extent of peak overlap in multidimensional NMR spectra and its validation using both, experimenta...
NMR structure note: PHD domain from death inducer obliterator protein and its interaction with H3K4me3 [0.03%]
PHDI蛋白的PHD结构域及其与H3K4me3相互作用的核磁共振结构
Clara M Santiveri,M Flor García-Mayoral,José M Pérez-Cañadillas et al.
Clara M Santiveri et al.
Sliding and target location of DNA-binding proteins: an NMR view of the lac repressor system [0.03%]
DNA结合蛋白的滑动与目标定位： Lac阻遏物系统的NMR视角
Karine Loth,Manuel Gnida,Julija Romanuka et al.
Karine Loth et al.
In non-specific lac headpiece-DNA complexes selective NMR line broadening is observed that strongly depends on length and composition of the DNA fragments. This broadening involves amide protons found in the non-specific lac-DNA structure t...
Nucleic acid helix structure determination from NMR proton chemical shifts [0.03%]
基于核磁共振氢化学位移的核酸螺旋结构测定
Ramon M van der Werf,Marco Tessari,Sybren S Wijmenga
Ramon M van der Werf
We present a method for de novo derivation of the three-dimensional helix structure of nucleic acids using non-exchangeable proton chemical shifts as sole source of experimental restraints. The method is called chemical shift de novo struct...
Combination of ¹⁵N reverse labeling and afterglow spectroscopy for assigning membrane protein spectra by magic-angle-spinning solid-state NMR: application to the multidrug resistance protein EmrE [0.03%]
结合15N反向标记和余晖光谱的膜蛋白魔角旋转固态NMR谱学指认方法及其在多药耐药蛋白EmrE中的应用
James R Banigan,Anindita Gayen,Nathaniel J Traaseth
James R Banigan
Magic-angle-spinning (MAS) solid-state NMR spectroscopy has emerged as a viable method to characterize membrane protein structure and dynamics. Nevertheless, the spectral resolution for uniformly labeled samples is often compromised by redu...
PARAssign--paramagnetic NMR assignments of protein nuclei on the basis of pseudocontact shifts [0.03%]
基于伪接触移位的蛋白质核参量磁共振.assignments
Simon P Skinner,Mois Moshev,Mathias A S Hass et al.
Simon P Skinner et al.
The use of paramagnetic NMR data for the refinement of structures of proteins and protein complexes is widespread. However, the power of paramagnetism for protein assignment has not yet been fully exploited. PARAssign is software that uses ...
Modulating alignment of membrane proteins in liquid-crystalline and oriented gel media by changing the size and charge of phospholipid bicelles [0.03%]
通过改变双层磷脂圆盘状胶束的大小和电荷调节膜蛋白在液晶相及定向凝胶基质中的排列方向
Justin L Lorieau,Alexander S Maltsev,John M Louis et al.
Justin L Lorieau et al.
We demonstrate that alignment of a structured peptide or small protein solubilized in mixed phospholipid:detergent micelles or bicelles, when embedded in a compressed gel or liquid crystalline medium, can be altered by either changing the p...
Structure of hen egg-white lysozyme solvated in TFE/water: a molecular dynamics simulation study based on NMR data [0.03%]
基于NMR数据的TFE/H2O中溶有溶菌酶结构的分子动力学模拟研究
Andreas P Eichenberger,Wilfred F van Gunsteren,Lorna J Smith
Andreas P Eichenberger
Various experimental studies of hen egg white lysozyme (HEWL) in water and TFE/water clearly indicate structural differences between the native state and TFE state of HEWL, e.g. the helical content of the protein in the TFE state is much hi...