Bingjie Ouyang,Lei Wang,Shuo Wan et al.
Bingjie Ouyang et al.
Divide and conquer is always best: sensitivity of methyl correlation experiments [0.03%]
分化制胜－甲基相关实验的灵敏度问题
Kaustubh Sinha,Linda Jen-Jacobson,Gordon S Rule
Kaustubh Sinha
The HMCM [CG]CBCA experiment (Tugarinov and Kay in J Am Chem Soc 125:13868-13878, 2003) correlates methyl carbon and proton shifts to Cγ, Cβ, and Cα resonances for the purpose of resonance assignments. The relative sensitivity of the HMC...
Fast and accurate fitting of relaxation dispersion data using the flexible software package GLOVE [0.03%]
快速而准确地使用GLOVE软件套件拟合弛豫分散数据
Kenji Sugase,Tsuyoshi Konuma,Jonathan C Lansing et al.
Kenji Sugase et al.
Relaxation dispersion spectroscopy is one of the most widely used techniques for the analysis of protein dynamics. To obtain a detailed understanding of the protein function from the view point of dynamics, it is essential to fit relaxation...
Biomolecular ligands screening using radiation damping difference WaterLOGSY spectroscopy [0.03%]
利用辐射弛豫差异WaterLOGSY谱学进行生物分子配体的筛选研究
Peng Sun,Xianwang Jiang,Bin Jiang et al.
Peng Sun et al.
Water-ligand observed via gradient spectroscopy (WaterLOGSY) is a widely used nuclear magnetic resonance method for ligand screening. The crucial procedure for the effectiveness of WaterLOGSY is selective excitation of the water resonance. ...
Protein backbone and sidechain torsion angles predicted from NMR chemical shifts using artificial neural networks [0.03%]
基于核磁共振化学位移的蛋白质骨干和侧链扭转角的人工神经网络预测方法
Yang Shen,Ad Bax
Yang Shen
A new program, TALOS-N, is introduced for predicting protein backbone torsion angles from NMR chemical shifts. The program relies far more extensively on the use of trained artificial neural networks than its predecessor, TALOS+. Validation...
Identifying inter-residue resonances in crowded 2D (13)C- (13)C chemical shift correlation spectra of membrane proteins by solid-state MAS NMR difference spectroscopy [0.03%]
利用固态MAS NMR差别谱学在拥挤的二维(13)C-(13)C化学位移相关谱中鉴别膜蛋白中的残基间共振峰
Yimin Miao,Timothy A Cross,Riqiang Fu
Yimin Miao
The feasibility of using difference spectroscopy, i.e. subtraction of two correlation spectra at different mixing times, for substantially enhanced resolution in crowded two-dimensional (13)C-(13)C chemical shift correlation spectra is pres...
Solution NMR study of the yeast cytochrome c peroxidase: cytochrome c interaction [0.03%]
酵母细胞色素c过氧化物酶-细胞色素c复合物的溶液NMR研究
Alexander N Volkov,Nico A J van Nuland
Alexander N Volkov
Here we present a solution NMR study of the complex between yeast cytochrome c (Cc) and cytochrome c peroxidase (CcP), a paradigm for understanding the biological electron transfer. Performed for the first time, the CcP-observed heteronucle...
Automated solid-state NMR resonance assignment of protein microcrystals and amyloids [0.03%]
蛋白质微晶和淀粉样蛋白的自动固态NMR共振分配
Elena Schmidt,Julia Gath,Birgit Habenstein et al.
Elena Schmidt et al.
Solid-state NMR is an emerging structure determination technique for crystalline and non-crystalline protein assemblies, e.g., amyloids. Resonance assignment constitutes the first and often very time-consuming step to a structure. We presen...
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology [0.03%]
利用动态核极化增强固态NMR作为核糖体结构生物学的新工具
Ioannis Gelis,Veronika Vitzthum,Neha Dhimole et al.
Ioannis Gelis et al.
The impact of Nuclear Magnetic Resonance (NMR) on studies of large macromolecular complexes hinges on improvements in sensitivity and resolution. Dynamic nuclear polarization (DNP) in the solid state can offer improved sensitivity, provided...
Mary J Harner,Andreas O Frank,Stephen W Fesik
Mary J Harner
Nuclear magnetic resonance (NMR) spectroscopy has evolved into a powerful tool for fragment-based drug discovery over the last two decades. While NMR has been traditionally used to elucidate the three-dimensional structures and dynamics of ...