Efficient protocol for backbone and side-chain assignments of large, intrinsically disordered proteins: transient secondary structure analysis of 49.2 kDa microtubule associated protein 2c [0.03%]
高效地对大的无序蛋白进行主链和侧链共振峰指认的实验方法：分析微管结合蛋白TAU（2C区）的瞬态二级结构动力学性质
Jiří Nováček,Lubomír Janda,Radka Dopitová et al.
Jiří Nováček et al.
Microtubule-associated proteins (MAPs) are abundantly present in axons and dendrites, and have been shown to play crucial role during the neuronal morphogenesis. The period of main dendritic outgrowth and synaptogenesis coincides with high ...
Geerten W Vuister,Rasmus H Fogh,Pieter M S Hendrickx et al.
Geerten W Vuister et al.
Biomolecular structures at atomic resolution present a valuable resource for the understanding of biology. NMR spectroscopy accounts for 11% of all structures in the PDB repository. In response to serious problems with the accuracy of some ...
13C structuring shifts for the analysis of model β-hairpins and β-sheets in proteins: diagnostic shifts appear only at the cross-strand H-bonded residues [0.03%]
用于蛋白质模型β-发卡和β-片层结构分析的13C重排移位：仅在交叉链氢键残基处出现诊断移位
Irene Shu,Michele Scian,James M Stewart et al.
Irene Shu et al.
The present studies have shown that (13)C=O, (13)C(α) and (13)C(β) of H-bonded strand residues in β-hairpins provide additional probes for quantitating the extent of folding in β-hairpins and other β-sheet models. Large differences in ...
Paramagnetic properties of the low- and high-spin states of yeast cytochrome c peroxidase [0.03%]
酵母细胞色素c过氧化物酶低自旋态和高自旋态的顺磁性性质
Sophie Vanwetswinkel,Nico A J van Nuland,Alexander N Volkov
Sophie Vanwetswinkel
Here we describe paramagnetic NMR analysis of the low- and high-spin forms of yeast cytochrome c peroxidase (CcP), a 34 kDa heme enzyme involved in hydroperoxide reduction in mitochondria. Starting from the assigned NMR spectra of a low-spi...
Quantitative one- and two-dimensional 13C spectra of microcrystalline proteins with enhanced intensity [0.03%]
增强强度的微晶蛋白的一维和二维定量碳－13光谱学研究
Rudra N Purusottam,Geoffrey Bodenhausen,Piotr Tekely
Rudra N Purusottam
We recorded quantitative, uniformly enhanced one- and two-dimensional (13)C spectra of labelled microcrystalline proteins. The approach takes advantage of efficient equilibration of magnetization by low-power proton irradiation using Phase ...
Out-and-back 13C-13C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS [0.03%]
利用超快魔角旋转固态NMR在质子检测下通过往返13C-13C旋轨转移进行蛋白质共振分配
Emeline Barbet-Massin,Andrew J Pell,Kristaps Jaudzems et al.
Emeline Barbet-Massin et al.
We present here (1)H-detected triple-resonance H/N/C experiments that incorporate CO-CA and CA-CB out-and-back scalar-transfer blocks optimized for robust resonance assignment in biosolids under ultra-fast magic-angle spinning (MAS). The fi...
Johannes Björnerås,Adolfo Botana,Gareth A Morris et al.
Johannes Björnerås et al.
Complex mixtures are at the heart of biology, and biomacromolecules almost always exhibit their function in a mixture, e.g., the mode of action for a spider venom is typically dependent on a cocktail of compounds, not just the protein. Info...
PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer [0.03%]
带有和不带PAR的PAIN：第三自旋辅助异核极化转移的变体
Vipin Agarwal,Mariana Sardo,Ingo Scholz et al.
Vipin Agarwal et al.
In this article, we describe third-spin assisted heteronuclear recoupling experiments, which play an increasingly important role in measuring long-range heteronuclear couplings, in particular (15)N-(13)C, in proteins. In the proton-assisted...
Partial alignment and measurement of residual dipolar couplings of proteins under high hydrostatic pressure [0.03%]
高压下的蛋白质部分对齐及残余偶极耦合的测定
Yinan Fu,A Joshua Wand
Yinan Fu
High-pressure NMR spectroscopy has emerged as a complementary approach for investigating various structural and thermodynamic properties of macromolecules. Noticeably absent from the array of experimental restraints that have been employed ...
Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-β peptide: perspectives for DNP [0.03%]
利用固态NMR对阿尔茨海默病淀粉样蛋白原纤维的研究及其动态核极化前景展望
Juan-Miguel Lopez del Amo,Dennis Schneider,Antoine Loquet et al.
Juan-Miguel Lopez del Amo et al.
Dynamic Nuclear Polarization solid-state NMR holds the potential to enable a dramatic increase in sensitivity by exploiting the large magnetic moment of the electron. However, applications to biological solids are hampered in uniformly isot...