Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy [0.03%]
基于固态NMR光谱法的极化优化实验（POE）确定膜蛋白在脂质双层中的结构拓扑性
Kaustubh R Mote,T Gopinath,Gianluigi Veglia
Kaustubh R Mote
The low sensitivity inherent to both the static and magic angle spinning techniques of solid-state NMR (ssNMR) spectroscopy has thus far limited the routine application of multidimensional experiments to determine the structure of membrane ...
Enhanced accuracy of kinetic information from CT-CPMG experiments by transverse rotating-frame spectroscopy [0.03%]
基于横向旋转框架谱学的CT-CPMG实验的动力学信息精度增强方法
David Ban,Adam Mazur,Marta G Carneiro et al.
David Ban et al.
Micro-to-millisecond motions of proteins transmit pivotal signals for protein function. A powerful technique for the measurement of these motions is nuclear magnetic resonance spectroscopy. One of the most widely used methodologies for this...
Resonance assignment for a particularly challenging protein based on systematic unlabeling of amino acids to complement incomplete NMR data sets [0.03%]
基于系统性氨基酸去标记以补充不完整核磁共振数据集的特别具有挑战性的蛋白质的共振指派
Peter Bellstedt,Thomas Seiboth,Sabine Häfner et al.
Peter Bellstedt et al.
NMR-based structure determination of a protein requires the assignment of resonances as indispensable first step. Even though heteronuclear through-bond correlation methods are available for that purpose, challenging situations arise in cas...
Direct correlation of consecutive C'-N groups in proteins: a method for the assignment of intrinsically disordered proteins [0.03%]
蛋白质中连续C'-N基团的直接相关性：一种用于指定固有无序蛋白的方法
David Pantoja-Uceda,Jorge Santoro
David Pantoja-Uceda
Two novel 3D (13)C-detected experiments, hNcocaNCO and hnCOcaNCO, are proposed to facilitate the resonance assignment of intrinsically disordered proteins. The experiments correlate the (15)N and (13)C' chemical shifts of two consecutive am...
Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins [0.03%]
带选择性的高效同核CO-CA交叉极化在蛋白质中的应用
Veniamin Chevelkov,Chaowei Shi,Hannes Klaus Fasshuber et al.
Veniamin Chevelkov et al.
Previously introduced for highly deuterated proteins, band-selective magnetization transfer between CO and CA spins by dipolar-based homonuclear cross polarization is applied here to a protonated protein. Robust and efficient recoupling is ...
Gijs van der Schot,Zaiyong Zhang,Robert Vernon et al.
Gijs van der Schot et al.
We report advances in the calculation of protein structures from chemical shift nuclear magnetic resonance data alone. Our previously developed method, CS-Rosetta, assembles structures from a library of short protein fragments picked from a...
Luciana E F Machado,Yulia Pustovalova,Andrew C Kile et al.
Luciana E F Machado et al.
SHPRH (SNF2, histone linker, PHD, RING, helicase) is a SWI2/SNF2-family ATP-dependent chromatin remodeling factor, and one of E3 ubiquitin ligases responsible for Ubc13-Mms2-dependent K63 poly-ubiquitination of PCNA (proliferating cell nucl...
Protein conformational exchange measured by 1H R1ρ relaxation dispersion of methyl groups [0.03%]
利用甲基1H R1ρ弛豫展宽测量蛋白质构象交换
Ulrich Weininger,Annica T Blissing,Janosch Hennig et al.
Ulrich Weininger et al.
Activated dynamics plays a central role in protein function, where transitions between distinct conformations often underlie the switching between active and inactive states. The characteristic time scales of these transitions typically fal...
Dynamics of exocyclic groups in the Escherichia coli O91 O-antigen polysaccharide in solution studied by carbon-13 NMR relaxation [0.03%]
采用碳-13核磁共振弛豫研究溶液中大肠杆菌O91脂多糖O抗原聚糖中外环状基团的动力学行为
Maria Soltesova,Jozef Kowalewski,Göran Widmalm
Maria Soltesova
Carbon-13 relaxation data are reported for exocyclic groups of hexopyranosyl sugar residues in the repeating unit within the Escherichia coli O91 O-antigen polysaccharide in a dilute D2O solution. The measurements of T 1, T 2 and heteronucl...
PDBStat: a universal restraint converter and restraint analysis software package for protein NMR [0.03%]
PDBStat：蛋白质NMR的通用约束转换器和约束分析软件包
Roberto Tejero,David Snyder,Binchen Mao et al.
Roberto Tejero et al.
The heterogeneous array of software tools used in the process of protein NMR structure determination presents organizational challenges in the structure determination and validation processes, and creates a learning curve that limits the br...