Reliability of exclusively NOESY-based automated resonance assignment and structure determination of proteins [0.03%]
完全基于NOESY的蛋白质自动共振分配和结构测定的可靠性
Elena Schmidt,Peter Güntert
Elena Schmidt
Protein structure determination by NMR can in principle be speeded up both by reducing the measurement time on the NMR spectrometer and by a more efficient analysis of the spectra. Here we study the reliability of protein structure determin...
EZ-ASSIGN, a program for exhaustive NMR chemical shift assignments of large proteins from complete or incomplete triple-resonance data [0.03%]
一个程序可对大型蛋白质进行NMR化学位移的全面指定，由完整或不完整的三级共振数据得出
Erik R P Zuiderweg,Ireena Bagai,Paolo Rossi et al.
Erik R P Zuiderweg et al.
For several of the proteins in the BioMagResBank larger than 200 residues, 60 % or fewer of the backbone resonances were assigned. But how reliable are those assignments? In contrast to complete assignments, where it is possible to check wh...
ORIUM: optimized RDC-based Iterative and Unified Model-free analysis [0.03%]
基于RDC的优化迭代和统一模型独立分析方法ORIUM
T Michael Sabo,Colin A Smith,David Ban et al.
T Michael Sabo et al.
Residual dipolar couplings (RDCs) are NMR parameters that provide both structural and dynamic information concerning inter-nuclear vectors, such as N-H(N) and Cα-Hα bonds within the protein backbone. Two approaches for extracting this inf...
Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR [0.03%]
溶液NMR稳定溶酶体肽转运蛋白TAPL膜嵌入靶向结构域构象的研究
Franz Tumulka,Christian Roos,Frank Löhr et al.
Franz Tumulka et al.
The ATP binding cassette transporter TAPL translocates cytosolic peptides into the lumen of lysosomes driven by the hydrolysis of ATP. Functionally, this transporter can be divided into coreTAPL, comprising the transport function, and an ad...
NMR spectroscopy reveals unexpected structural variation at the protein-protein interface in MHC class I molecules [0.03%]
核磁共振光谱揭示了MHC I类分子在蛋白质-蛋白质界面上出乎意料的结构变化
Monika Beerbaum,Martin Ballaschk,Natalja Erdmann et al.
Monika Beerbaum et al.
β2-Microglobulin (β2m) is a small, monomorphic protein non-covalently bound to the heavy chain (HC) in polymorphic major histocompatibility complex (MHC) class I molecules. Given the high evolutionary conservation of structural features o...
Practical considerations over spectral quality in solid state NMR spectroscopy of soluble proteins [0.03%]
关于溶液中蛋白质固态NMR谱质量的实用考量因素
Marco Fragai,Claudio Luchinat,Giacomo Parigi et al.
Marco Fragai et al.
Great theoretical and methodological advances are pushing the limits of resolution and sensitivity in solid state NMR (SSNMR). However, sample preparation remains a critical issue for the success of an experiment. The factors affecting spec...
Selectively dispersed isotope labeling for protein structure determination by magic angle spinning NMR [0.03%]
选择性分散同位素标记用于魔角旋转固体核磁共振蛋白质结构测定
Matthew T Eddy,Marina Belenky,Astrid C Sivertsen et al.
Matthew T Eddy et al.
The power of nuclear magnetic resonance spectroscopy derives from its site-specific access to chemical, structural and dynamic information. However, the corresponding multiplicity of interactions can be difficult to tease apart. Complimenta...
Improved chemical shift based fragment selection for CS-Rosetta using Rosetta3 fragment picker [0.03%]
基于化学位移的CS-Rosetta片段选择的改进方法
Robert Vernon,Yang Shen,David Baker et al.
Robert Vernon et al.
A new fragment picker has been developed for CS-Rosetta that combines beneficial features of the original fragment picker, MFR, used with CS-Rosetta, and the fragment picker, NNMake, that was used for purely sequence based fragment selectio...
Structural and dynamical characterization of the Miz-1 zinc fingers 5-8 by solution-state NMR [0.03%]
溶液状态下NMR测定Miz-1 C2H2型锌指蛋白5-8结构与动力学特性
David Bernard,Mikaël Bédard,Josée Bilodeau et al.
David Bernard et al.
Myc-interacting zinc finger protein-1 (Miz-1) is a BTB/POZ transcription factor that activates the transcription of cytostatic genes, such as p15(INK4B) or p21(CIP1). The C-terminus of Miz-1 contains 13 consensus C2H2 zinc finger domains (Z...
4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs [0.03%]
一种用于13C,15N标记RNA顺序指认的4D非均匀采样C,C-NOESY实验
Jan Stanek,Peter Podbevšek,Wiktor Koźmiński et al.
Jan Stanek et al.
A 4D (13)C(aromatic),(13)C(ribose)-edited NOESY experiment is introduced to improve sequential assignment of non-coding RNA, often hampered by a limited dispersion of (1)H and (13)C chemical shifts. The (13)C-labeling of RNA is fully utiliz...