Julien Orts,Beat Vögeli,Roland Riek et al.
Julien Orts et al.
Recently developed methods to measure distances in proteins with high accuracy by "exact" nuclear Overhauser effects (eNOEs) make it possible to determine stereospecific assignments, which are particularly important to fully exploit the acc...
Equivalence between Euler angle conventions for the description of tensorial interactions in liquid NMR: application to different software programs [0.03%]
用于液态NMR张量相互作用描述的欧拉角约定之间的等价性：不同软件程序的应用
Patrice Dosset,Philippe Barthe,Martin Cohen-Gonsaud et al.
Patrice Dosset et al.
Long-range orientational restraints derived from alignment or rotational diffusion tensors have greatly contributed to the expansion of applications in biomolecular NMR. The orientation of the principal axis system of these tensors is usual...
Resonance assignment of the NMR spectra of disordered proteins using a multi-objective non-dominated sorting genetic algorithm [0.03%]
利用多目标非支配排序遗传算法对无序蛋白质核磁共振谱的共振峰指派问题进行求解
Yu Yang,Keith J Fritzsching,Mei Hong
Yu Yang
A multi-objective genetic algorithm is introduced to predict the assignment of protein solid-state NMR (SSNMR) spectra with partial resonance overlap and missing peaks due to broad linewidths, molecular motion, and low sensitivity. This non...
Amplitudes and time scales of picosecond-to-microsecond motion in proteins studied by solid-state NMR: a critical evaluation of experimental approaches and application to crystalline ubiquitin [0.03%]
固态NMR研究蛋白皮秒至微秒运动的幅度和时间尺度：实验方法评价及泛素晶体的应用
Jens D Haller,Paul Schanda
Jens D Haller
Solid-state NMR provides insight into protein motion over time scales ranging from picoseconds to seconds. While in solution state the methodology to measure protein dynamics is well established, there is currently no such consensus protoco...
Independent valine and leucine isotope labeling in Escherichia coli protein overexpression systems [0.03%]
大肠杆菌蛋白高效表达体系中独立的缬氨酸和亮氨酸同位素标记技术研究
Roman J Lichtenecker,Katharina Weinhäupl,Lukas Reuther et al.
Roman J Lichtenecker et al.
The addition of labeled α-ketoisovalerate to the growth medium of a protein-expressing host organism has evolved into a versatile tool to achieve concomitant incorporation of specific isotopes into valine- and leucine- residues. The result...
Specific labeling and assignment strategies of valine methyl groups for NMR studies of high molecular weight proteins [0.03%]
用于高分子量蛋白质核磁共振研究的缬氨酸甲基群的特异性标记和指派策略
Guillaume Mas,Elodie Crublet,Olivier Hamelin et al.
Guillaume Mas et al.
The specific protonation of valine and leucine methyl groups in proteins is typically achieved by overexpressing proteins in M9/D2O medium supplemented with either labeled α-ketoisovalerate for the labeling of the four prochiral methyl gro...
Kuo-Wei Hung,Chan-Chia Hsu,Chin Yu
Kuo-Wei Hung
Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids [0.03%]
利用立体选择性甲基化氨基酸通过大肠杆菌细胞表达对蛋白质中的亮氨酸和缬氨酸残基进行差异同位素标记
Yohei Miyanoiri,Mitsuhiro Takeda,Kosuke Okuma et al.
Yohei Miyanoiri et al.
The (1)H-(13)C HMQC signals of the (13)CH3 moieties of Ile, Leu, and Val residues, in an otherwise deuterated background, exhibit narrow line-widths, and thus are useful for investigating the structures and dynamics of larger proteins. This...
HN(CA)N and HN(COCA)N experiments for assignment of large disordered proteins [0.03%]
HN(CA)N和HN(COCA)N实验在大无序蛋白共振分配中的应用
Xiao Liu,Daiwen Yang
Xiao Liu
Two new 3D HN-based experiments are proposed for backbone assignment of large disordered proteins. The spectra obtained with the new pulse schemes are free of redundant diagonal peaks (HiNi-Ni) and provide sequential correlations (HiNi-Ni+1...
Internal protein dynamics on ps to μs timescales as studied by multi-frequency (15)N solid-state NMR relaxation [0.03%]
利用多频(15)N固态NMR弛豫研究蛋白质内部动力学（-ps到μs时间尺度-）
Tatiana Zinkevich,Veniamin Chevelkov,Bernd Reif et al.
Tatiana Zinkevich et al.
A comprehensive analysis of the dynamics of the SH3 domain of chicken alpha-spectrin is presented, based upon (15)N T1 and on- and off-resonance T1ρ relaxation times obtained on deuterated samples with a partial back-exchange of labile pro...