Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR [0.03%]
固态核磁在脂质体中对酵母OST亚基Ost4及其关键突变体Ost4V23D的复性和化学位移指认
Bharat P Chaudhary,Jochem Struppe,Hem Moktan et al.
Bharat P Chaudhary et al.
N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively call...
Kieran T Cockburn,Brian D Sykes
Kieran T Cockburn
The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19F to determine the orientation of fluorine labeled biomolecules in situ in oriented biological systems such as muscle. The difficulty with a sing...
A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy [0.03%]
人胚肾293细胞中选择性氨基酸15N标记的全面评估用于核磁共振光谱学
Ganesh P Subedi,Elijah T Roberts,Alexander R Davis et al.
Ganesh P Subedi et al.
A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. Thus, mammalian expression systems are often employed to characterize structure/function relationships using NMR spectr...
Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale [0.03%]
超越两态蛋白质构象交换的慢速CEST：应用于毫秒时间尺度上的三态蛋白质相互转化
Ved Prakash Tiwari,Debajyoti De,Nemika Thapliyal et al.
Ved Prakash Tiwari et al.
Although NMR spectroscopy is routinely used to study the conformational dynamics of biomolecules, robust analyses of the data are challenged in cases where exchange is more complex than two-state, such as when a 'visible' major conformer ex...
Pauline Defant,Christof Regl,Christian G Huber et al.
Pauline Defant et al.
Reducing sugars can spontaneously react with free amines in protein side chains leading to posttranslational modifications (PTMs) called glycation. In contrast to glycosylation, glycation is a non-enzymatic modification with consequences on...
Short and long range 2D 15N-15N NMR correlations among peptide groups by novel solid state dipolar mixing schemes [0.03%]
利用新的固体状态偶极去相方案研究肽段之间的短程和长程15N-15N二维核磁共振谱学相关性
Sungsool Wi,Conggang Li,Karen Pham et al.
Sungsool Wi et al.
A recently developed homonuclear dipolar recoupling scheme, Adiabatic Linearly FREquency Swept reCOupling (AL FRESCO), was applied to record two-dimensional (2D) 15N-15N correlations on uniformly 15N-labeled GB1 powders. A major feature exp...
19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor [0.03%]
不同去污剂和纳米盘对A2A腺苷受体的影响的19F-NMR研究
Francisco Mendoza-Hoffmann,Canyong Guo,Yanzhuo Song et al.
Francisco Mendoza-Hoffmann et al.
For the A2A adenosine receptor (A2AAR), a class A G-protein-coupled receptor (GPCR), reconstituted in n-dodecyl-β-D-maltoside (DDM)/cholesteryl hemisuccinate (CHS) mixed micelles, previous 19F-NMR studies revealed the presence of...
Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae [0.03%]
在酿酒酵母中表达的均匀13C/15N标记和糖基化的人IgG1 Fc片段
Alexander R Davis,Elijah T Roberts,I Jonathan Amster et al.
Alexander R Davis et al.
Despite the prevalence and importance of glycoproteins in human biology, methods for isotope labeling suffer significant limitations. Common prokaryotic platforms do not produce mammalian post-translation modifications that are essential to...
Efficient determination of the accessible conformation space of multi-domain complexes based on EPR PELDOR data [0.03%]
基于EPR PELDOR数据有效确定多域复合物可访问构象空间的方法
Sina Kazemi,Anna Lopata,Andreas Kniss et al.
Sina Kazemi et al.
Many proteins can adopt multiple conformations which are important for their function. This is also true for proteins and domains that are covalently linked to each other. One important example is ubiquitin, which can form chains of differe...
Alexander Klein,Suresh K Vasa,Rasmus Linser
Alexander Klein
1H-detected solid-state NMR spectroscopy has been becoming increasingly popular for the characterization of protein structure, dynamics, and function. Recently, we showed that higher-dimensionality solid-state NMR spectroscopy can aid reson...