COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript [0.03%]
COLMARvista：一种用于JavaScript的二维和伪三维NMR光谱处理、可视化和分析软件
Dawei Li,Rafael Brüschweiler
Dawei Li
COLMARvista is presented as a new, highly versatile software for the easy and intuitive processing and visual inspection of 2D and pseudo-3D NMR data both for uniformly and non-uniformly sampled datasets. COLMARvista allows fully autonomous...
Quantifying protein-drug lifetimes in human cells by 19F NMR spectroscopy [0.03%]
通过19F核磁共振光谱量化人体细胞中蛋白质-药物的寿命
Wenkai Zhu,Fatema Bhinderwala,Sarah Rambo et al.
Wenkai Zhu et al.
The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged in intricate networks of communication that modulate binary protein-ligand/protein interactions. As a result, it is b...
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles [0.03%]
一套完整的交叉相关弛豫实验以确定蛋白质主链的二面角
Paulina Bartosińska-Marzec,Bartłomiej Banaś,Clemens Kauffmann et al.
Paulina Bartosińska-Marzec et al.
The investigation of structural propensities of proteins is essential for understanding how they function at the molecular level. NMR, offering atomic-scale information, is often the method of choice. One of the available techniques relies ...
Terminal spin labeling of xylotriose strongly affects interactions in the active site of xylanase BcX [0.03%]
木三糖的末端旋轉標記對木聚糖酶BcX活性位點內的相互作用影響很大
Mahin Saberi,René Dekkers,Leonardo Passerini et al.
Mahin Saberi et al.
Paramagnetic probes provide long-range distance information and report on minor conformations of biomacromolecules. However, it is important to realize that any probe can affect the system of interest. Here, we report on the effects of atta...
Finn O&#x;Dea,Aiden J Seargeant,Jessica Hurcum et al.
Finn O&#x;Dea et al.
Addition of glycine betaine up to 1 M gave rise to increased intensity for some weak signals in the HSQC spectra of barnase and Plasmodium falciparum flap endonuclease. The signals that were enhanced were low intensity signals, often from a...
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies [0.03%]
以丙酮酸作为唯一碳源的细菌培养基的生化特征研究及其在核磁共振中的应用探索
Çağdaş Dağ,Kerem Kahraman
Çağdaş Dağ
The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different c...
Counterintuitive method improves yields of isotopically labelled proteins expressed in flask-cultured Escherichia coli [0.03%]
反直觉的方法提高了培养罐中表达的同位素标记蛋白质的产量
Miguel Ángel Treviño
Miguel Ángel Treviño
NMR is a powerful tool for the structural and dynamic study of proteins. One of the necessary conditions for the study of these proteins is their isotopic labelling with 15N and 13C. One of the most widely used methods to obtain these label...
Local structure propensities in disordered proteins from cross-correlated NMR spin relaxation [0.03%]
无序蛋白质中来自交叉相关核磁共振自旋弛豫的局部结构倾向性
Daniel Braun,Clemens Kauffmann,Andreas Beier et al.
Daniel Braun et al.
Structurally diverse ensembles of intrinsically disordered proteins or regions are difficult to determine, because experimental observables usually report a conformational average. Therefore, in order to infer the underlying distribution, a...
Reducing experimental time through spin-lattice relaxation enhancement via dissolved oxygen [0.03%]
通过溶解氧增强自旋晶格弛豫以减少实验时间
Louis-Philippe Picard,Dmitry Pichugin,Shuya Kate Huang et al.
Louis-Philippe Picard et al.
Large proteins and dilute spin systems within a deuterated background are often characterized by long proton (1H) spin-lattice relaxation times (T1), which directly impacts the recycle delay and hence, the total experimental time. Dioxygen ...
Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding [0.03%]
采用哈达玛编码对组氨酸、精氨酸和赖氨酸进行固态NMR谱编辑
Tata Gopinath,Alyssa Kraft,Kyungsoo Shin et al.
Tata Gopinath et al.
The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their detection can be complicated due to spectral overlap as well as conformational and hydrogen exchange. In this work, we ...