A CON-based NMR assignment strategy for pro-rich intrinsically disordered proteins with low signal dispersion: the C-terminal domain of histone H1.0 as a case study [0.03%]
一种基于CON的NMR分配策略，用于富含脯氨酸的内在无序蛋白质（信号分散低）：组蛋白H1.0 C末端结构域为例研究
Belén Chaves-Arquero,David Pantoja-Uceda,Alicia Roque et al.
Belén Chaves-Arquero et al.
The C-terminal domain of histone H1.0 (C-H1.0) is involved in DNA binding and is a main determinant of the chromatin condensing properties of histone H1.0. Phosphorylation at the (S/T)-P-X-(K/R) motifs affects DNA binding and is crucial for...
Joint X-ray/NMR structure refinement of multidomain/multisubunit systems [0.03%]
多结构域/多亚基系统的联合X射线/NMR结构优化
Azzurra Carlon,Enrico Ravera,Giacomo Parigi et al.
Azzurra Carlon et al.
Data integration in structural biology has become a paradigm for the characterization of biomolecular systems, and it is now accepted that combining different techniques can fill the gaps in each other's blind spots. In this frame, one of t...
15N transverse relaxation measurements for the characterization of µs-ms dynamics are deteriorated by the deuterium isotope effect on 15N resulting from solvent exchange [0.03%]
溶剂交换导致的15N去簇效应损害了用于表征微秒至毫秒时间尺度过程的15N横向弛豫测量结果
Pratibha Kumari,Lukas Frey,Alexander Sobol et al.
Pratibha Kumari et al.
15N R2 relaxation measurements are key for the elucidation of the dynamics of both folded and intrinsically disordered proteins (IDPs). Here we show, on the example of the intrinsically disordered protein α-synuclein and the folded domain ...
A methyl 1H double quantum CPMG experiment to study protein conformational exchange [0.03%]
一种甲基1H双量子CPMG实验用于研究蛋白质构象交换
Anusha B Gopalan,Tairan Yuwen,Lewis E Kay et al.
Anusha B Gopalan et al.
Protein conformational changes play crucial roles in enabling function. The Carr-Purcell-Meiboom-Gill (CPMG) experiment forms the basis for studying such dynamics when they involve the interconversion between highly populated and sparsely f...
Conformational exchange of aromatic side chains by 1H CPMG relaxation dispersion [0.03%]
利用CPMG弛豫展宽法检测芳香族侧链的构象交换
Heiner N Raum,Matthias Dreydoppel,Ulrich Weininger
Heiner N Raum
Aromatic side chains are attractive probes of protein dynamics on the millisecond time scale, because they are often key residues in enzyme active sites and protein binding sites. Further they allow to study specific processes, like histidi...
Direct amide 15N to 13C transfers for solid-state assignment experiments in deuterated proteins [0.03%]
用于氘代蛋白质固态分配实验的直接酰胺氮-十三碳转移方法
Alons Lends,Francesco Ravotti,Giorgia Zandomeneghi et al.
Alons Lends et al.
The assignment of protein backbone and side-chain NMR chemical shifts is the first step towards the characterization of protein structure. The recent introduction of proton detection in combination with fast MAS has opened up novel opportun...
3-O-Methyl-D-glucose mutarotation and proton exchange rates assessed by 13C, 1H NMR and by chemical exchange saturation transfer and spin lock measurements [0.03%]
基于NMR以及化学交换饱和转移和自旋锁测量的D-葡萄糖甲基苷互变异构和质子交换速率研究
Michal Rivlin,Gil Navon
Michal Rivlin
3-O-Methyl-D-glucose (3OMG) was recently suggested as an agent to image tumors using chemical exchange saturation transfer (CEST) MRI. To characterize the properties of 3OMG in solution, the anomeric equilibrium and the mutarotation rates o...
Rapid automated determination of chemical shift anisotropy values in the carbonyl and carboxyl groups of fd-y21m bacteriophage using solid state NMR [0.03%]
利用固态NMR快速自动测定FD-Y21M噬菌体羰基和羧基化学位移各向异值
Tom Aharoni,Amir Goldbourt
Tom Aharoni
Determination of chemical shift anisotropy (CSA) in immobilized proteins and protein assemblies is one of several tools to determine protein dynamics on the timescales of microseconds and faster. The large CSA values of C=O groups in the ri...
Correlated motions of C'-N and Cα-Cβ pairs in protonated and per-deuterated GB3 [0.03%]
GB3的质子化和完全去污化的C'-N和Cα-Cβ对的相关运动
Liliya Vugmeyster,Aaron Griffin,Dmitry Ostrovsky et al.
Liliya Vugmeyster et al.
We investigated correlated µs-ms time scale motions of neighboring 13C'-15N and 13Cα-13Cβ nuclei in both protonated and perdeuterated samples of GB3. The techniques employed, NMR relaxation due to cross-correlated chemical shift modulati...
On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies [0.03%]
甲硫基球拟酵母在人源G蛋白偶联受体NMR实验异位表达及同位素标记中的应用研究
Lindsay Clark,Igor Dikiy,Daniel M Rosenbaum et al.
Lindsay Clark et al.
NMR studies of human integral membrane proteins provide unique opportunities to probe structure and dynamics at specific locations and on multiple timescales, often with significant implications for disease mechanism and drug development. S...