Susanne Penzel,Andres Oss,Mai-Liis Org et al.
Susanne Penzel et al.
We report linewidth and proton T1, T1ρ and T2' relaxation data of the model protein ubiquitin acquired at MAS frequencies up to 126 kHz. We find a predominantly linear improvement in linewidths and coherence decay times of protons with inc...
Exchangeable deuterons introduce artifacts in amide 15N CEST experiments used to study protein conformational exchange [0.03%]
交换氘原子会在用于研究蛋白质构象变化的酰胺15N CEST实验中引入伪像
Ved Prakash Tiwari,Subhendu Pandit,Pramodh Vallurupalli
Ved Prakash Tiwari
Protein molecules sample different conformations in solution and characterizing these conformations is crucial to understanding protein function. 15N CEST experiments are now routinely used to study slow conformational exchange of protein m...
Increasing the buffering capacity of minimal media leads to higher protein yield [0.03%]
增加最小培养基的缓冲能力可提高蛋白质产量
Stephan B Azatian,Navneet Kaur,Michael P Latham
Stephan B Azatian
We describe a general and simple modification to the standard M9 minimal medium recipe that leads to an approximate twofold increase in the yield of heterologously expressed proteins in Escherichia coli BL21(DE3) bacteria. We monitored the ...
Assessing the potential of quantitative 2D HSQC NMR in 13C enriched living organisms [0.03%]
定量二维HSQC核磁共振在13C富集的活体生物中的潜在应用评估
Daniel Lane,Thomas E Skinner,Naum I Gershenzon et al.
Daniel Lane et al.
In vivo Nuclear Magnetic Resonance (NMR) spectroscopy has great potential to interpret the biochemical response of organisms to their environment, thus making it an essential tool in understanding toxic mechanisms. However, magnetic suscept...
NMR-STAR: comprehensive ontology for representing, archiving and exchanging data from nuclear magnetic resonance spectroscopic experiments [0.03%]
NMR-STAR：核磁共振谱学实验的数据表示、归档及交换的全面本体论
Eldon L Ulrich,Kumaran Baskaran,Hesam Dashti et al.
Eldon L Ulrich et al.
The growth of the biological nuclear magnetic resonance (NMR) field and the development of new experimental technology have mandated the revision and enlargement of the NMR-STAR ontology used to represent experiments, spectral and derived d...
CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion [0.03%]
一种魔法角旋转NMR探针，在转子爆炸时可限制样品(CONFINE-MAS)
Thomas Wiegand,Andreas Hunkeler,Alexander Däpp et al.
Thomas Wiegand et al.
Magic-angle spinning (MAS) is mandatory in solid-state NMR experiments to achieve resolved spectra. In rare cases, instabilities in the rotation or damage of either the rotor or the rotor cap can lead to a so called "rotor crash" involving ...
Oligomeric transition and dynamics of RNA binding by the HuR RRM1 domain in solution [0.03%]
溶液中HuRRRM1结构域的寡聚过渡和RNA结合动力学研究
Carolina Lixa,Amanda Mujo,Mariana T Q de Magalhães et al.
Carolina Lixa et al.
Human antigen R (HuR) functions as a major post-transcriptional regulator of gene expression through its RNA-binding activity. HuR is composed by three RNA recognition motifs, namely RRM1, RRM2, and RRM3. The two N-terminal RRM domains are ...
Selective suppression of excipient signals in 2D 1H-13C methyl spectra of biopharmaceutical products [0.03%]
生物制药产品二维1H-13C甲基光谱中辅料信号的选择性抑制
Luke W Arbogast,Frank Delaglio,Joel R Tolman et al.
Luke W Arbogast et al.
While the use of 1H-13C methyl correlated NMR spectroscopy at natural isotopic abundance has been demonstrated as feasible on protein therapeutics as large as monoclonal antibodies, spectral interference from aliphatic excipients remains a ...
BEST and SOFAST experiments for resonance assignment of histidine and tyrosine side chains in 13C/15N labeled proteins [0.03%]
用于半胱氨酸和酪氨酸侧链共振 assignments 的 BEST 和 SOFAST 实验
Nina Eleni Christou,Bernhard Brutscher
Nina Eleni Christou
Aromatic amino-acid side chains are essential components for the structure and function of proteins. We present herein a set of NMR experiments for time-efficient resonance assignment of histidine and tyrosine side chains in uniformly 13C/1...
Alexander Klein,Suresh K Vasa,Rasmus Linser
Alexander Klein
Given that solid-state NMR is being used for protein samples of increasing molecular weight and complexity, higher-dimensionality methods are likely to be more and more indispensable for unambiguous chemical shift assignments in the near fu...