Accuracy and precision of protein structures determined by magic angle spinning NMR spectroscopy: for some 'with a little help from a friend' [0.03%]
魔角旋转核磁共振技术测定蛋白质结构的精确度和精准度：“有些需要朋友的帮助”
Ryan W Russell,Matthew P Fritz,Jodi Kraus et al.
Ryan W Russell et al.
We present a systematic investigation into the attainable accuracy and precision of protein structures determined by heteronuclear magic angle spinning solid-state NMR for a set of four proteins of varied size and secondary structure conten...
Arthur G Palmer rd
Arthur G Palmer rd
Gerhard Wagner
Gerhard Wagner
NMR: an essential structural tool for integrative studies of T cell development, pMHC ligand recognition and TCR mechanobiology [0.03%]
核磁共振：T细胞发育、pMHC配体识别及TCR力学生物学的整合研究的重要结构工具
Robert J Mallis,Kristine N Brazin,Jonathan S Duke-Cohan et al.
Robert J Mallis et al.
Early studies of T cell structural biology using X-ray crystallography, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) focused on a picture of the αβT cell receptor (αβTCR) component domains and their cognate...
Hybridization of TEDOR and NCX MAS solid-state NMR experiments for simultaneous acquisition of heteronuclear correlation spectra and distance measurements [0.03%]
结合TEDOR和NCX MAS固态NMR实验同时获取异核相关光谱和距离信息
T Gopinath,Songlin Wang,John Lee et al.
T Gopinath et al.
Magic angle spinning (MAS) solid-state NMR (ssNMR) spectroscopy is a major technique for the characterization of the structural dynamics of biopolymers at atomic resolution. However, the intrinsic low sensitivity of this technique poses sig...
Artifacts can emerge in spectra recorded with even the simplest of pulse schemes: an HMQC case study [0.03%]
以HMQC为例说明即使是简单的脉冲序列记录的谱图中也可能存在伪峰问题
Lewis E Kay
Lewis E Kay
With the development of sophisticated pulsed field gradient- and phase cycling-approaches for suppressing certain coherence transfer pathways and selecting for others it is sometimes easy to forget that the process is not flawless. In some ...
Jonas Fredriksson,Wolfgang Bermel,Martin Billeter
Jonas Fredriksson
A flexible and scalable approach for protein NMR is introduced that builds on rapid data collection via projection spectroscopy and analysis of the spectral input data via joint decomposition. Input data may originate from various types of ...
Kumar Tekwani Movellan,Eszter E Najbauer,Supriya Pratihar et al.
Kumar Tekwani Movellan et al.
We describe a new labeling method that allows for full protonation at the backbone Hα position, maintaining protein side chains with a high level of deuteration. We refer to the method as alpha proton exchange by transamination (α-PET) si...
The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins [0.03%]
同位素标记蛋白质的N末端葡萄糖酸化的核磁共振特征分析及其广泛性研究
David Schweida,Pierre Barraud,Christof Regl et al.
David Schweida et al.
N-terminal gluconoylation is a moderately widespread modification in recombinant proteins expressed in Escherichia coli, in particular in proteins bearing an N-terminal histidine-tag. This post-translational modification has been investigat...
Biosynthetic production of fully carbon-13 labeled retinal in E. coli for structural and functional studies of rhodopsins [0.03%]
用于视紫红蛋白结构和功能研究的全十三碳标记视黄醛在大肠杆菌中的生物合成生产
Rachel A Munro,Jeffrey de Vlugt,Meaghan E Ward et al.
Rachel A Munro et al.
The isomerization of a covalently bound retinal is an integral part of both microbial and animal rhodopsin function. As such, detailed structure and conformational changes in the retinal binding pocket are of significant interest and are st...