Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach [0.03%]
基于整合结构生物学方法鉴定膜蛋白插入酶BamA的构象特异性纳米抗体
Hundeep Kaur,Jean-Baptiste Hartmann,Roman P Jakob et al.
Hundeep Kaur et al.
The insertase BamA is an essential protein of the bacterial outer membrane. Its 16-stranded transmembrane β-barrel contains a lateral gate as a key functional element. This gate is formed by the C-terminal half of the last β-strand. The B...
Correction to: Joint X-ray/NMR structure refinement of multidomain/multisubunit systems [0.03%]
多结构域/多亚基系统的联锁X射线/核磁共振结构精修的更正
Azzurra Carlon,Enrico Ravera,Giacomo Parigi et al.
Azzurra Carlon et al.
The article "Joint X-ray/NMR structure refinement of multidomain/multisubunit systems" written by "Azzurra Carlon, Enrico Ravera, Giacomo Parigi, Garib N. Murshudov and Claudio Luchinat" was originally published electronically on the publis...
Published Erratum
Journal of biomolecular NMR. 2019 Jul;73(6-7):279. DOI:10.1007/s10858-019-00238-4 2019
Time-domain signal modelling in multidimensional NMR experiments for estimation of relaxation parameters [0.03%]
利用多维核磁共振实验进行信号建模以估计弛豫参数的方法
Yevgen Matviychuk,Mark J Bostock,Daniel Nietlispach et al.
Yevgen Matviychuk et al.
We present a model-based method for estimation of relaxation parameters from time-domain NMR data specifically suitable for processing data in popular 2D phase-sensitive experiments. Our model is formulated in terms of commutative bicomplex...
Maximizing accuracy of RNA structure in refinement against residual dipolar couplings [0.03%]
最大化残余各向异性强耦合约束下的RNA结构精修的精确性
Christina Bergonzo,Alexander Grishaev
Christina Bergonzo
Structural information about ribonucleic acid (RNA) is lagging behind that of proteins, in part due to its high charge and conformational variability. Molecular dynamics (MD) has played an important role in describing RNA structure, complem...
Improving the sensitivity of FT-NMR spectroscopy by apodization weighted sampling [0.03%]
通过加权采样改善傅里叶变换核磁共振谱仪灵敏度
Bernd Simon,Herbert Köstler
Bernd Simon
Apodization weighted acquisition is a simple approach to enhance the sensitivity of multidimensional NMR spectra by scaling the number of scans during acquisition of the indirect dimension(s). The signal content of the resulting spectra is ...
Measurement of residual dipolar couplings in methyl groups via carbon detection [0.03%]
基于碳探测的甲基团残留二重耦合测量方法
Robert V Williams,Jeong-Yeh Yang,Kelley W Moremen et al.
Robert V Williams et al.
Residual dipolar couplings (RDCs) provide both structural and dynamical information useful in the characterization of biological macromolecules. While most data come from the interaction of simple pairs of directly bonded spin-1/2 nuclei (1...
Charles F DeLisle,H Bhagya Mendis,Justin L Lorieau
Charles F DeLisle
Spectral resolution remains one of the most significant limitations in the NMR study of biomolecules. We present the srNOESY (super resolution nuclear overhauser effect spectroscopy) experiment, which enhances the resolution of NOESY cross-...
Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands [0.03%]
选择性同位素标记用于与非标记配体结合的蛋白质的NMR结构测定
Konstantinos Tripsianes,Ulrike Schütz,Leonidas Emmanouilidis et al.
Konstantinos Tripsianes et al.
The physiological role of proteins is frequently linked to interactions with non-protein ligands or posttranslational modifications. Structural characterization of these complexes or modified proteins by NMR may be difficult as the ligands ...
Characterization of H/D exchange in type 1 pili by proton-detected solid-state NMR and molecular dynamics simulations [0.03%]
利用固态氢/脱uterium交换结合质子检测及分子动力学模拟表征I型菌毛的结构动态學性质
Songhwan Hwang,Carl Öster,Veniamin Chevelkov et al.
Songhwan Hwang et al.
Uropathogenic Escherichia coli invades and colonizes hosts by attaching to cells using adhesive pili on the bacterial surface. Although many biophysical techniques have been used to study the structure and mechanical properties of pili, man...
19F-substituted amino acids as an alternative to fluorophore labels: monitoring of degradation and cellular uptake of analogues of penetratin by 19F NMR [0.03%]
19F标记的氨基酸作为荧光探针替代物的初步研究：利用19F NMR监测pepstatin类似物的降解及穿膜肽penetratin类似物的细胞摄取过程
Malene V Christensen,Kenneth T Kongstad,Teis Esben Sondergaard et al.
Malene V Christensen et al.
Current methods for assessment of cellular uptake of cell-penetrating peptides (CPPs) often rely on detection of fluorophore-labeled CPPs. However, introduction of the fluorescent probe often confers changed physicochemical properties, so t...