Improving the quality of oriented membrane protein spectra using heat-compensated separated local field experiments [0.03%]
利用热补偿局部分辨场实验提高膜蛋白质量异质性谱图的品质
Songlin Wang,T Gopinath,Gianluigi Veglia
Songlin Wang
Oriented sample solid-state NMR (OS-ssNMR) spectroscopy is a powerful technique to determine the topology of membrane proteins in oriented lipid bilayers. Separated local field (SLF) experiments are central to this technique as they provide...
Manman Lu,Rieko Ishima,Tatyana Polenova et al.
Manman Lu et al.
We present 19F longitudinal and transverse relaxation studies for four differently fluorosubstituted L-tryptophans, which carry single F atoms in the indole ring, both in the context of the free amino acid and when located in the cyclophili...
Darón I Freedberg,Lewis E Kay
Darón I Freedberg
Investigation of the internal structure and dynamics of cellulose by 13C-NMR relaxometry and 2DPASS-MAS-NMR measurements [0.03%]
采用碳-13核磁共振弛豫和二维平面偏振谱固体核磁共振研究纤维素的内部结构与动力学性质
Manasi Ghosh,Naveen Kango,Krishna Kishor Dey
Manasi Ghosh
Internal structure and dynamics of commercial and natural cellulose were studied by measuring chemical shift anisotropy (CSA) parameters, and spin-lattice relaxation rate (1/T1) at each and every chemically different carbon nuclear site. CS...
Accounting for the temperature dependence of 13C spin-lattice relaxation of methyl groups in the glycyl-alanyl-leucine model system under MAS with spin diffusion [0.03%]
利用自旋扩散的MAS在甘氨酰-丙氨酰-亮氨酸模型体系中考虑甲基组的13C自旋晶格弛豫温度效应
Van C Phan,Elizabeth A Fry,Kurt W Zilm
Van C Phan
The difficulties in quantitatively modeling the temperature dependence of spin-lattice relaxation in a model isotope-enriched peptide are explored as a prelude to obtaining dynamics parameters for motions in proteins from such measurements....
Lucila Andrea Acevedo,Nathan E Korson,Justin M Williams et al.
Lucila Andrea Acevedo et al.
Peptidyl Prolyl Isomerases (PPIases) accelerate cis-trans isomerization of prolyl peptide bonds. In rice, the PPIase LRT2 is essential for lateral root initiation. LRT2 displays in vitro isomerization of a highly conserved W-P peptide bond ...
Partial alignment, residual dipolar couplings and molecular symmetry in solution NMR [0.03%]
溶液NMR中的部分定相、剩余各向异性耦合及分子对称性
Justin L Lorieau
Justin L Lorieau
Residual dipolar couplings (RDCs) and residual anisotropic chemical shifts (RACSs) are produced by the partial alignment of solution NMR samples. RDCs and RACSs yield high-resolution structural and dynamic information on the orientation of ...
Determination of methyl order parameters using solid state NMR under off magic angle spinning [0.03%]
利用非神奇角度旋转固态NMR确定甲基顺序参数
Kai Xue,Salvatore Mamone,Benita Koch et al.
Kai Xue et al.
Quantification of dipolar couplings in biological solids is important for the understanding of dynamic processes. Under Magic Angle Spinning (MAS), order parameters are normally obtained by recoupling of anisotropic interactions involving t...
Michelle L Gill,Andrew Hsu,Arthur G Palmer
Michelle L Gill
The zero- and double-quantum methyl TROSY Hahn-echo and the methyl 1H-1H dipole-dipole cross-correlation nuclear magnetic resonance experiments enable estimation of multiple quantum chemical exchange broadening in methyl groups in proteins....
Exchange saturation transfer and associated NMR techniques for studies of protein interactions involving high-molecular-weight systems [0.03%]
高分子量体系蛋白质作用的交换饱和转移及核磁共振技术研究进展
Vitali Tugarinov,G Marius Clore
Vitali Tugarinov
A brief overview of theoretical and experimental aspects of the Dark state Exchange Saturation Transfer (DEST) and lifetime line broadening ([Formula: see text]) NMR methodologies is presented from a physico-chemical perspective. We describ...