Talia Fargason,Ting Wang,Naiduwadura Ivon Upekala De Silva et al.
Talia Fargason et al.
Residual dipolar couplings (RDCs) provide valuable NMR parameters that can be used for structural calculation and verification. Measuring RDCs requires aligning macromolecules using one of various types of alignment media. Of different alig...
Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water [0.03%]
利用超极化水增强蛋白质三维和碳检测二维核磁共振灵敏度
Gregory L Olsen,Or Szekely,Borja Mateos et al.
Gregory L Olsen et al.
Signal enhancements of up to two orders of magnitude in protein NMR can be achieved by employing HDO as a vector to introduce hyperpolarization into folded or intrinsically disordered proteins. In this approach, hyperpolarized HDO produced ...
Slow ring flips in aromatic cluster of GB1 studied by aromatic 13C relaxation dispersion methods [0.03%]
利用芳香族碳-13弛豫展宽法研究GB1中芳香簇的缓慢环翻转现象
Matthias Dreydoppel,Heiner N Raum,Ulrich Weininger
Matthias Dreydoppel
Ring flips of phenylalanine and tyrosine are a hallmark of protein dynamics. They report on transient breathing motions of proteins. In addition, flip rates also depend on stabilizing interactions in the ground state, like aromatic stacking...
Mass spectrometry assisted arginine side chains assignment of NMR resonances in natural abundance proteins [0.03%]
基于质谱的精胺侧链磁共振特征辅助天然蛋白质丰度.assign.py
Jingjing Lu,Fengmei Zhou,Wanhui Liu et al.
Jingjing Lu et al.
Arginine side chains play critical roles in many protein-ligand interactions and enzyme catalysis. Unambiguous resonance assignment is a prerequisite for the nuclear magnetic resonance (NMR) spectroscopy studies of arginine side chains dyna...
Amino-acid selective isotope labeling enables simultaneous overlapping signal decomposition and information extraction from NMR spectra [0.03%]
氨基酸特异的同位素标记技术可同时实现NMR谱图中重叠信号的拆分与信息提取
Takuma Kasai,Shunsuke Ono,Seizo Koshiba et al.
Takuma Kasai et al.
Signal overlapping is a major bottleneck for protein NMR analysis. We propose a new method, stable-isotope-assisted parameter extraction (SiPex), to resolve overlapping signals by a combination of amino-acid selective isotope labeling (AASI...
Boosting the resolution of low-field [Formula: see text] relaxation experiments on intrinsically disordered proteins with triple-resonance NMR [0.03%]
利用三共振核磁共振技术提高内在无序蛋白质低场[NCAA: see text]弛豫实验的分辨率
Zuzana Jaseňáková,Vojtěch Zapletal,Petr Padrta et al.
Zuzana Jaseňáková et al.
Improving our understanding of nanosecond motions in disordered proteins requires the enhanced sampling of the spectral density function obtained from relaxation at low magnetic fields. High-resolution relaxometry and two-field NMR measurem...
Determining methyl sidechain conformations in a CS-ROSETTA model using methyl 1H-13C residual dipolar couplings [0.03%]
基于甲基1H-13C残差偶极耦合确定CS-ROSETTA模型中的甲基侧链构象
Theodoros K Karamanos,Vitali Tugarinov,G Marius Clore
Theodoros K Karamanos
Modelling of protein structures based on backbone chemical shifts, using programs such as CS-ROSETTA, is becoming increasingly popular, especially for systems where few restraints are available or where homologous structures are already kno...
Dispersion from Cα or NH: 4D experiments for backbone resonance assignment of intrinsically disordered proteins [0.03%]
基于Cα或NH的散射：用于固有无序蛋白主链共振 assignments的4D实验
Helena Tossavainen,Santeri Salovaara,Maarit Hellman et al.
Helena Tossavainen et al.
Resonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the ami...
Two-dimensional NMR lineshape analysis of single, multiple, zero and double quantum correlation experiments [0.03%]
单量子、多量子、零量子及双量子相关实验的二维核磁共振谱型分析
Christopher A Waudby,Margaux Ouvry,Ben Davis et al.
Christopher A Waudby et al.
NMR spectroscopy provides a powerful approach for the characterisation of chemical exchange and molecular interactions by analysis of series of experiments acquired over the course of a titration measurement. The appearance of NMR resonance...
Optimizing fluorine labelling for 19F solid-state NMR in oriented biological systems [0.03%]
优化氟标记以用于定向生物系统中的19F固态NMR研究
Ian M Robertson,Brittney A Klein,Brian D Sykes
Ian M Robertson
When planning a fluorine labeling strategy for 19F solid state NMR (ssNMR) studies of the structure and/or mobility of fluorine labeled compounds in situ in an oriented biological system, it is important to characterize the NMR properties o...