Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision [0.03%]
一种提高PFG-NMR扩散数据处理精度的新方案DDfit及其网络服务器发布
Vladislav A Salikov,Olga O Lebedenko,Nikolai R Skrynnikov et al.
Vladislav A Salikov et al.
In this communication we describe a new scheme to process the data from stimulated echo protein diffusion experiments. For a series of gradient-encoded proton spectra [Formula: see text] considered over the selected spectral region [Formula...
Single spin exact gradients for the optimization of complex pulses and pulse sequences [0.03%]
用于复杂脉冲和脉冲序列优化的单自旋精确梯度
Stella Slad,Burkhard Luy
Stella Slad
The efficient computer optimization of magnetic resonance pulses and pulse sequences involves the calculation of a problem-adapted cost function as well as its gradients with respect to all controls applied. The gradients generally can be c...
Conformation-selective detection of residues in solid proteins under magic-angle-spinning [0.03%]
固态魔角旋转条件下蛋白质变体的选择性构象检测
Pragyan P Parida,Pravin P Taware,Kaustubh R Mote
Pragyan P Parida
We demonstrate here a pulse sequence based on rotational-echo double resonance (REDOR) that can help distinguish resonances based on the ψ-torsion angle, which allows it to distinguish α-helical and β-sheet regions in solid proteins unde...
Toward site-specific characterization of structural perturbations on glycosylated Fc using NMR at natural abundance [0.03%]
基于天然丰度的NMR技术在糖基化Fc位点特异性结构表征中的应用
Béatrice Vibert,Sarah Nguyen,Faustine Henot et al.
Béatrice Vibert et al.
Monoclonal antibodies (mAbs) are leading therapeutic agents due to their high specificity and limited side effects. Ensuring their structural integrity under stress and maintaining batch consistency require robust quality control. Methyl 2D...
A fast and efficient strategy for the NMR assignment of Fab methyl groups [0.03%]
一种快速高效的Fab甲基核磁共振指认方法
Faustine Henot,Béatrice Vibert,Arthur Giraud et al.
Faustine Henot et al.
Owing to their high specificity and therapeutic effectiveness, monoclonal antibodies (mAbs) have rapidly become one of the leading classes of biologic drugs used to treat critical illnesses. The antigen-binding fragment (Fab) of mAbs plays ...
19F-NMR spectroscopy of fluorinated isoleucine analogues in a protein [0.03%]
氟化异亮氨酸类似物的蛋白质中的氟磁共振光谱研究
Adarshi P Welegedara,Yi Jiun Tan,Matteo Borgini et al.
Adarshi P Welegedara et al.
The R3H domain of the human protein Sµbp-2 was produced with 5-fluoro-L-isoleucine (FIle) and 5,5-difluoro-L-isoleucine (diFIle) as probes for detection by 19F-NMR spectroscopy. The fluorinated protein, produced by cell-free protein synthe...
Incorporating the BEST methodology in experiments for measuring paramagnetic relaxation enhancements [0.03%]
将BEST方法学用于测量顺磁松弛增效作用的实验中
Nikolaus M Loening
Nikolaus M Loening
Band-selective excitation short-transient (BEST) sequences are widely used for protein NMR experiments that start with amide proton magnetization, such as 1H-15N HSQC, 1H-15N TROSY, and multidimensional backbone assignment experiments, beca...
Correction: Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning [0.03%]
订正：在105kHz全质子魔角旋转条件下测定蛋白质长程接触
Zainab O Mustapha,Eren H Ozturk,Benjamin E Lefkin et al.
Zainab O Mustapha et al.
Published Erratum
Journal of biomolecular NMR. 2025 Nov 21. DOI:10.1007/s10858-025-00478-7 2025
Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in 1H-detected protein solid-state NMR [0.03%]
利用简单的生物表达方法对蛋白质进行侧链选择性氘标记可提高固态NMR谱分辨率并简化谱图
Yoshiki Shigemitsu,Yuki Miyazaki,Hibiki Terami et al.
Yoshiki Shigemitsu et al.
We present a novel approach for side-chain-selective deuteration of proteins to improve 1Hα spectral resolution and to simplify side-chain signals in 1H-detected protein solid-state NMR (SSNMR) with a simple bio-expression method using E. ...
Correction: Quantifying protein-drug lifetimes in human cells by 19F NMR spectroscopy [0.03%]
关于校正：通过19F NMR光谱量化人体细胞中的蛋白质-药物寿命
Wenkai Zhu,Fatema Bhinderwala,Sarah Rambo et al.
Wenkai Zhu et al.
Published Erratum
Journal of biomolecular NMR. 2025 Oct 8. DOI:10.1007/s10858-025-00473-y 2025