Fluorine NMR functional screening: from purified enzymes to human intact living cells [0.03%]
含氟核磁功能筛选：从纯化酶到活细胞中的应用研究
Claudio Dalvit,Marina Veronesi,Anna Vulpetti
Claudio Dalvit
The substrate- or cofactor-based fluorine NMR screening, also known as n-FABS (n fluorine atoms for biochemical screening), represents a powerful method for performing a direct functional assay in the search of inhibitors or enhancers of an...
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning [0.03%]
利用极化优化实验（POE）进行多接收固态NMR光谱研究在超快准各向同性旋转条件下观测分子内氢键网络的拓扑学结构及其动态变化规律
T Gopinath,Daniel K Weber,Gianluigi Veglia
T Gopinath
Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experim...
Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments [0.03%]
通过抑制伪迹实现甲基分子中自旋纯化的弛豫实验效应
Fa-An Chao,Domarin Khago,R Andrew Byrd
Fa-An Chao
Recent methyl adiabatic relaxation dispersion experiments provide examination of conformational dynamics across a very wide timescale (102-105 s-1) and, particularly, provide insight into the hydrophobic core of proteins and allosteric effe...
Revisiting biomolecular NMR spectroscopy for promoting small-molecule drug discovery [0.03%]
生物分子核磁共振光谱在小分子药物发现中的应用及其前景
Hiroyuki Hanzawa,Takashi Shimada,Mizuki Takahashi et al.
Hiroyuki Hanzawa et al.
Recently, there has been increasing interest in new modalities such as therapeutic antibodies and gene therapy at a number of pharmaceutical companies. Moreover, in small-molecule drug discovery at such companies, efforts have focused on ha...
Understanding and solving abnormal peak splitting in 3D HCCH-TOCSY and HCC(CO)NH-TOCSY [0.03%]
三维HCCH-TOCSY和HCC（CO）NH-TOCSY异常峰裂解析及解决
Youlin Xia,Tairan Yuwen,Paolo Rossi
Youlin Xia
The 3D HCCH-TOCSY and HCC(CO)NH-TOCSY experiments provide through bond connectivity and are used for side-chain chemical shift assignment by solution-state NMR. Careful design and implementation of the pulse sequence are key to the successf...
A novel high-dimensional NMR experiment for resolving protein backbone dihedral angle ambiguities [0.03%]
一种用于解决蛋白质主链二面角歧义性的新型高维核磁共振实验
Clemens Kauffmann,Krzysztof Kazimierczuk,Thomas C Schwarz et al.
Clemens Kauffmann et al.
Intrinsically disordered proteins (IDPs) are challenging established structural biology perception and urge a reassessment of the conventional understanding of the subtle interplay between protein structure and dynamics. Due to their import...
Pinpoint analysis of a protein in slow exchange using F1F2-selective ZZ-exchange spectroscopy: assignment and kinetic analysis [0.03%]
利用F1F2选择性ZZ交换谱法分析慢交换蛋白的精确定量及动力学分析
Mayu Nishizawa,Erik Walinda,Daichi Morimoto et al.
Mayu Nishizawa et al.
ZZ-exchange spectroscopy is widely used to study slow exchange processes in biomolecules, especially determination of exchange rates and assignment of minor peaks. However, if the exchange cross peaks overlap or the populations are skewed, ...
MAS NMR detection of hydrogen bonds for protein secondary structure characterization [0.03%]
利用氢核磁共振波谱法检测氢键以表征蛋白质二级结构
Daniel Friedrich,Jacqueline Perodeau,Andrew J Nieuwkoop et al.
Daniel Friedrich et al.
Hydrogen bonds are essential for protein structure and function, making experimental access to long-range interactions between amide protons and heteroatoms invaluable. Here we show that measuring distance restraints involving backbone hydr...
Xue Kang,Wancheng Zhao,Malitha C Dickwella Widanage et al.
Xue Kang et al.
Carbohydrates are essential to various life activities in living organisms and serve as the central component in many biomaterials. As an emerging technique with steadily improving resolution, solid-state Nuclear Magnetic Resonance (NMR) sp...
Two-dimensional 19F-13C correlation NMR for 19F resonance assignment of fluorinated proteins [0.03%]
用于蛋白质氟化共振 ASSIGNMENT 的二维~(19)F-~(13)C相关核磁共振谱法
Alexander A Shcherbakov,Matthias Roos,Byungsu Kwon et al.
Alexander A Shcherbakov et al.
19F solid-state NMR is an excellent approach for measuring long-range distances for structure determination and for studying molecular motion. For multi-fluorinated proteins, assignment of 19F chemical shifts has been traditionally carried ...