Towards autonomous analysis of chemical exchange saturation transfer experiments using deep neural networks [0.03%]
基于深度神经网络的化学交换饱和转移实验自分析方法研究
Gogulan Karunanithy,Tairan Yuwen,Lewis E Kay et al.
Gogulan Karunanithy et al.
Macromolecules often exchange between functional states on timescales that can be accessed with NMR spectroscopy and many NMR tools have been developed to characterise the kinetics and thermodynamics of the exchange processes, as well as th...
Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins [0.03%]
配体诱导的结构转变结合顺磁离子有助于大蛋白质中甲基集团的NMR鉴定
Lars Mühlberg,Tuncay Alarcin,Thorben Maass et al.
Lars Mühlberg et al.
NMR spectroscopy allows the study of biomolecules in close-to-native conditions. Structural information can be inferred from the NMR spectra when an assignment is available. Protein assignment is usually a time-consuming task, being special...
Fundamental and practical aspects of machine learning for the peak picking of biomolecular NMR spectra [0.03%]
机器学习在生物大分子NMR谱峰识别中的理论与实际问题探讨
Da-Wei Li,Alexandar L Hansen,Lei Bruschweiler-Li et al.
Da-Wei Li et al.
Rapid progress in machine learning offers new opportunities for the automated analysis of multidimensional NMR spectra ranging from protein NMR to metabolomics applications. Most recently, it has been demonstrated how deep neural networks (...
77Se-13C based dipolar correlation experiments to map selenium sites in microcrystalline proteins [0.03%]
利用77Se-13C构建偶极相关实验在微晶蛋白质中寻找硒位置
Caitlin M Quinn,Shiping Xu,Guangjin Hou et al.
Caitlin M Quinn et al.
Sulfur-containing sites in proteins are of great importance for both protein structure and function, including enzymatic catalysis, signaling pathways, and recognition of ligands and protein partners. Selenium-77 is an NMR active spin-1/2 n...
Art Palmer
Art Palmer
Optimization and validation of multi-state NMR protein structures using structural correlations [0.03%]
基于结构相关性多构象NMR蛋白质结构的优化与验证
Dzmitry Ashkinadze,Harindranath Kadavath,Roland Riek et al.
Dzmitry Ashkinadze et al.
Recent advances in the field of protein structure determination using liquid-state NMR enable the elucidation of multi-state protein conformations that can provide insight into correlated and non-correlated protein dynamics at atomic resolu...
Site-specific protein methyl deuterium quadrupolar patterns by proton-detected 3D 2H-13C-1H MAS NMR spectroscopy [0.03%]
位点特异性蛋白质甲基氘四极图案的质子检测三维2H-13C-1H固体魔角旋转核磁共振光谱学研究
Ümit Akbey
Ümit Akbey
Determination of protein structure and dynamics is key to understand the mechanism of protein action. Perdeuterated proteins have been used to obtain high resolution/sensitivty NMR experiments via proton-detection. These methods utilizes 1H...
Matthias Dreydoppel,Jochen Balbach,Ulrich Weininger
Matthias Dreydoppel
NMR-spectroscopy has certain unique advantages for recording unfolding transitions of proteins compared e.g. to optical methods. It enables per-residue monitoring and separate detection of the folded and unfolded state as well as possible e...
Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1 [0.03%]
无谷氨酰胺哺乳动物细胞表达均一化学生物素标记的重组糖蛋白及其在人源IgG1药用糖型NMR分析中的应用研究
Saeko Yanaka,Hirokazu Yagi,Rina Yogo et al.
Saeko Yanaka et al.
Mammalian cells are widely used for producing recombinant glycoproteins of pharmaceutical interest. However, a major drawback of using mammalian cells is the high production costs associated with uniformly isotope-labeled glycoproteins due ...
Protein resonance assignment by solid-state NMR based on 1H-detected 13C double-quantum spectroscopy at fast MAS [0.03%]
快速MAS基于1H检测的13C双量子谱的固态核磁共振蛋白共振指派
Alons Lends,Mélanie Berbon,Birgit Habenstein et al.
Alons Lends et al.
Solid-state NMR spectroscopy is a powerful technique to study insoluble and non-crystalline proteins and protein complexes at atomic resolution. The development of proton (1H) detection at fast magic-angle spinning (MAS) has considerably in...