Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks [0.03%]
血清和尿液的生物样本库和代谢组学研究的前期处理标准操作程序
Patrizia Bernini,Ivano Bertini,Claudio Luchinat et al.
Patrizia Bernini et al.
(1)H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks....
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system [0.03%]
基于乳腺癌模型系统的缺氧诱导的代谢转变的核磁共振代谢组标志物识别研究
Aalim M Weljie,Alla Bondareva,Ping Zang et al.
Aalim M Weljie et al.
Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics techno...
Nelly Aranibar,Michael Borys,Nancy A Mackin et al.
Nelly Aranibar et al.
NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NM...
Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification [0.03%]
二维HMQC-COSY联用法在小分子化学位移指认及化合物鉴定中的应用
Kaifeng Hu,William M Westler,John L Markley
Kaifeng Hu
Chemical shift assignment is the first step toward the structure elucidation of natural products and other chemical compounds. We propose here the use of 2D concurrent HMQC-COSY as an experiment for rapid chemical shift assignment of small ...
Towards automatic metabolomic profiling of high-resolution one-dimensional proton NMR spectra [0.03%]
面向高分辨率一维氢质子NMR谱的代谢轮廓的自动分析法研究进展
Pascal Mercier,Michael J Lewis,David Chang et al.
Pascal Mercier et al.
Nuclear magnetic resonance (NMR) and Mass Spectroscopy (MS) are the two most common spectroscopic analytical techniques employed in metabolomics. The large spectral datasets generated by NMR and MS are often analyzed using data reduction te...
Metabolic profiling of vitamin C deficiency in Gulo-/- mice using proton NMR spectroscopy [0.03%]
利用核磁共振技术对Gulo-/-小鼠进行维生素C缺乏的代谢轮廓分析
Gavin E Duggan,B Joan Miller,Frank R Jirik et al.
Gavin E Duggan et al.
Nutrient deficiencies are an ongoing problem in many populations and ascorbic acid is a key vitamin whose mild or acute absence leads to a number of conditions including the famously debilitating scurvy. As such, the biochemical effects of ...
Fast methionine-based solution structure determination of calcium-calmodulin complexes [0.03%]
快速测定钙调素-钙复合物的甲硫氨酸基溶液结构
Jessica L Gifford,Hiroaki Ishida,Hans J Vogel
Jessica L Gifford
Here we present a novel NMR method for the structure determination of calcium-calmodulin (Ca(2+)-CaM)-peptide complexes from a limited set of experimental restraints. A comparison of solved CaM-peptide structures reveals invariability in Ca...
Metabolic profiles show specific mitochondrial toxicities in vitro in myotube cells [0.03%]
代谢轮廓显示肌管细胞体外培养的特定线粒体毒性
Qiuwei Xu,Heather Vu,Liping Liu et al.
Qiuwei Xu et al.
Mitochondrial toxicity has been a serious concern, not only in preclinical drug development but also in clinical trials. In mitochondria, there are several distinct metabolic processes including fatty acid β-oxidation, the tricarboxylic ac...
Metabolic characterization of Palatinate German white wines according to sensory attributes, varieties, and vintages using NMR spectroscopy and multivariate data analyses [0.03%]
用于表征普法尔茨德国白葡萄酒代谢特征的核磁共振波谱和多变量数据分析方法（按照感官属性、品种和年份）
Kashif Ali,Federica Maltese,Reinhard Toepfer et al.
Kashif Ali et al.
(1)H NMR (nuclear magnetic resonance spectroscopy) has been used for metabolomic analysis of 'Riesling' and 'Mueller-Thurgau' white wines from the German Palatinate region. Diverse two-dimensional NMR techniques have been applied for the id...
NMR-based stable isotope resolved metabolomics in systems biochemistry [0.03%]
基于核磁共振的稳定同位素解析代谢组学在系统生物化学中的应用
Teresa W-M Fan,Andrew N Lane
Teresa W-M Fan
An important goal of metabolomics is to characterize the changes in metabolic networks in cells or various tissues of an organism in response to external perturbations or pathologies. The profiling of metabolites and their steady state conc...