Characterization of a New Allelic Variant of Triosephosphate Isomerase from the LNCaP Human Prostate Cancer Cell Line: Enzyme Inhibition and Spectroscopic Studies [0.03%]
来自人前列腺癌LNCaP细胞系的新三磷酸甘油醛异构酶等位基因变异体的表征：酶抑制和光谱研究
Valeria Guzmán-Luna,Leticia Olvera-Rodríguez,Peniel Bustamante-Villalobos et al.
Valeria Guzmán-Luna et al.
Background: The glycolytic pathway plays an important role in tumor cells. Triosephosphate isomerase (TIM) catalyzes the reversible isomerization of D-glyceraldehyde-3-phosphate (GAP) to dihydroxyacetone phosphate (DHAP) ...
Targeting HIV-1 Envelope Proteins Using a Fragment Discovery All-Atom Computational Algorithm [0.03%]
靶向HIV-1包膜蛋白的片段发现全原子计算算法
Michael H Peters
Michael H Peters
Introduction: HIV viral envelope proteins are targets for small inhibitor molecules aimed at disrupting the cellular entry process. Potential peptide-class inhibitor molecules (rDNA drugs) have been previously identified,...
An Additional Method for Analyzing the Reversible Inhibition of an Enzyme Using Acid Phosphatase as a Model [0.03%]
一种分析酶的可逆抑制作用的新方法——以酸性磷酸酶为例
Jordan M Baumhardt,Benjamin M Dorsey,Craig C McLauchlan et al.
Jordan M Baumhardt et al.
Using wheat germ acid phosphatase and sodium orthovanadate as a competitive inhibitor, a novel method for analyzing reversible inhibition was carried out. Our alternative approach involves plotting the initial velocity at which product is f...
Beyond Thymidylate Synthase and Dihydrofolate Reductase: Impact of Non-coding microRNAs in Anticancer Chemoresistance [0.03%]
叶酸通路以外：非编码微小RNA在肿瘤化疗耐药中的作用
Jingfang Ju
Jingfang Ju
Chemoresistance is one of the major reasons for the failure of anticancer chemotherapy in treating advanced stage cancer. The mechanism of chemoresistance to fluoropyrimidines and antifolates has been extensively investigated in the past 40...
Inhibition Studies on Enzymes Involved in Isoprenoid Biosynthesis: Focus on Two Potential Drug Targets: DXR and IDI-2 Enzymes [0.03%]
异戊二烯生物合成酶的抑制研究：两个潜在药物靶标DXR和IDI-2酶的研究重点
Jérôme de Ruyck,Johan Wouters,C Dale Poulter
Jérôme de Ruyck
Isoprenoid compounds constitute an immensely diverse group of acyclic, monocyclic and polycyclic compounds that play important roles in all living organisms. Despite the diversity of their structures, this plethora of natural products arise...
Xinping Yue,Bin Shan,Joseph A Lasky
Xinping Yue
Pulmonary fibrosis is characterized by epithelial cell injury, accumulation of myofibroblasts, and excessive deposition of collagen and other extracellular matrix elements, leading to loss of pulmonary function. Studies in both humans and a...
Umesh K Jinwal,John Koren rd,Chad A Dickey
Umesh K Jinwal
Imbalanced protein load within cells is a critical aspect for most diseases of aging. In particular, the accumulation of proteins into neurotoxic aggregates is a common thread for a host of neurodegenerative diseases. Recent work demonstrat...
Clostridium difficile DNA polymerase IIIC: basis for activity of antibacterial compounds [0.03%]
难辨梭菌DNA聚合酶IIIC：抗菌化合物活性的基础
Andrea Torti,Andrea Lossani,Lida Savi et al.
Andrea Torti et al.
Based on the finding that aerobic Gram-positive antibacterials that inhibit DNA polymerase IIIC (pol IIIC) were potent inhibitors of the growth of anaerobic Clostridium difficile (CD) strains, we chose to clone and express the gene for pol ...
Inhibiting Glutathione Metabolism in Lung Lining Fluid as a Strategy to Augment Antioxidant Defense [0.03%]
抑制肺表面液体中谷胱甘肽代谢以增强抗氧化防御的策略
Martin Joyce-Brady,Jun Hiratake
Martin Joyce-Brady
Glutathione is abundant in the lining fluid that bathes the gas exchange surface of the lung. On the one hand glutathione in this extracellular pool functions in antioxidant defense to protect cells and proteins in the alveolar space from o...
Hyehun Choi,Kyan J Allahdadi,Rita C A Tostes et al.
Hyehun Choi et al.
Diacylglycerol kinases (DGKs), a family of lipid kinases, convert diacylglycerol (DG) to phosphatidic acid (PA). Acting as a second messenger, DG activates protein kinase C (PKC). PA, a signaling lipid, regulates diverse functions involved ...