David J Miller
David J Miller
This obituary provides some detail and background to the life and work of a pioneer of muscle physiology, Rolf Niedergerke, who died in December 2011 aged 90. His name is perhaps most widely known for his role in the discovery of the slidin...
Tetanic force potentiation of mouse fast muscle is shortening speed dependent [0.03%]
小鼠快肌的 tetanic 力增强与缩短速度相关
William Gittings,Jian Huang,Rene Vandenboom
William Gittings
The activity dependent potentiation of peak isometric force associated with phosphorylation of the myosin regulatory light chain (RLC) is generally restricted to low activation frequencies. The purpose of this study was to determine if musc...
Robert Simmons
Robert Simmons
Andrew G Szent-Györgyi,Clive R Bagshaw
Andrew G Szent-Györgyi
Improvement of the yields of recombinant actin and myosin V-HMM in the insect cell/baculovirus system by the addition of nutrients to the high-density cell culture [0.03%]
通过向高密度昆虫细胞培养中添加营养物质提高在昆虫细胞杆状病毒系统中重组actin和肌球蛋白V-HMM的产量
Takashi Ohki,Sergey V Mikhailenko,Tomomi Arai et al.
Takashi Ohki et al.
Baculovirus infection of Sf9 cells at high densities, such as during mid- and late exponential phase, often results in a significant reduction of protein yield per cell, compared to the early exponential phase. Nutrient depletion has been c...
Tuning the structural coupling between the transmembrane and cytoplasmic domains of phospholamban to control sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) function [0.03%]
通过调节磷脂酶蛋白的结构结合来控制肌浆网Ca(2+)-ATP酶（SERCA）的功能
Kim N Ha,Martin Gustavsson,Gianluigi Veglia
Kim N Ha
Phospholamban (PLN) is the endogenous inhibitor of the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), the integral membrane enzyme responsible for 70 % of the removal of Ca(2+) from the cytosol, inducing cardiac muscle relaxation in hu...
Expression of tyrosine kinase receptors in cultured dorsal root ganglion neurons in the presence of monosialoganglioside and skeletal muscle cells [0.03%]
单唾液酸神经节苷脂和骨骼肌细胞对培养的背根神经节神经元酪氨酸激酶受体表达的影响
Hao Li,Weiwei Zhang,Guixiang Liu et al.
Hao Li et al.
The neurotrophic factor-like activity of monosialoganglioside (GM1) has been shown to activate tyrosine kinase receptors (Trk). Targets of neuronal innervation play a vital role in regulating the survival and differentiation of innervating ...
Brett A Colson,Simon J Gruber,David D Thomas
Brett A Colson
We have used site-directed spectroscopic probes to detect structural changes, motions, and interactions due to phosphorylation of proteins involved in the regulation of muscle contraction and relaxation. Protein crystal structures provide s...
Temperature dependent measurements reveal similarities between muscle and non-muscle myosin motility [0.03%]
温度相关的测量揭示了肌肉和非肌肉肌球蛋白运动的相似性
Christopher M Yengo,Yasuharu Takagi,James R Sellers
Christopher M Yengo
We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in...