Increasing expression level and copy number of a Yarrowia lipolytica plasmid through regulated centromere function [0.03%]
通过调节着丝粒功能提高安琪酵母质粒的表达水平和拷贝数
Leqian Liu,Peter Otoupal,Anny Pan et al.
Leqian Liu et al.
Manipulating gene expression using different types of plasmids (centromeric, 2-micron, and autonomously replicating sequence) can expand expression levels and enable a quick characterization of combinations, both of which are highly desired...
Florian David,Verena Siewers
Florian David
Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and...
Genomic insights into the evolution of industrial yeast species Brettanomyces bruxellensis [0.03%]
基因组学见解：工业酵母物种巴特诺米耶氏酒香酵母的进化研究
Christopher D Curtin,Isak S Pretorius
Christopher D Curtin
Brettanomyces bruxellensis, like its wine yeast counterpart Saccharomyces cerevisiae, is intrinsically linked with industrial fermentations. In wine, B. bruxellensis is generally considered to contribute negative influences on wine quality,...
Environmental azole fungicide, prochloraz, can induce cross-resistance to medical triazoles in Candida glabrata [0.03%]
环境曲霉 fungicide，prochloraz可以诱导Candida glabrata对医学三唑的交叉抗性
Isabel Faria-Ramos,Pedro R Tavares,Sofia Farinha et al.
Isabel Faria-Ramos et al.
Acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. The main goal of this study was to assess the development of cross-resistance between agricultura...
Description of Kuraishia piskuri f.a., sp. nov., a new methanol assimilating yeast and transfer of phylogenetically related Candida species to the genera Kuraishia and Nakazawaea as new combinations [0.03%]
甲醇同化酵母菌克鲁亚西亚氏毕赤酵母的新种及其相关 Candida 种类向新属克鲁亚西亚氏属和鸟巢穗尾酵母属的转属研究
Cletus P Kurtzman,Christie J Robnett
Cletus P Kurtzman
The new anamorphic yeast Kuraishia piskuri, f.a., sp. nov. is described for three strains that were isolated from insect frass from trees growing in Florida, USA (type strain, NRRL YB-2544, CBS 13714). Species placement was based on phyloge...
The yeast oligopeptide transporter Opt2 is localized to peroxisomes and affects glutathione redox homeostasis [0.03%]
寡肽转运蛋白Opt2定位于过氧化物酶体并影响谷胱甘肽红ox平衡
Yael Elbaz-Alon,Bruce Morgan,Anne Clancy et al.
Yael Elbaz-Alon et al.
Glutathione, the most abundant small-molecule thiol in eukaryotic cells, is synthesized de novo solely in the cytosol and must subsequently be transported to other cellular compartments. The mechanisms of glutathione transport into and out ...
Yeast synthetic biology for the production of recombinant therapeutic proteins [0.03%]
酵母合成生物学在治疗用重组蛋白生产中的应用
Hyunah Kim,Su Jin Yoo,Hyun Ah Kang
Hyunah Kim
The production of recombinant therapeutic proteins is one of the fast-growing areas of molecular medicine and currently plays an important role in treatment of several diseases. Yeasts are unicellular eukaryotic microbial host cells that of...
A novel role of the ferric reductase Cfl1 in cell wall integrity, mitochondrial function, and invasion to host cells in Candida albicans [0.03%]
新型铁还原酶Cfl1在白色念珠菌细胞壁完整性、线粒体功能和侵入宿主细胞中的作用
Qilin Yu,Yijie Dong,Ning Xu et al.
Qilin Yu et al.
Candida albicans is an important opportunistic pathogen, causing both superficial mucosal infections and life-threatening systemic diseases. Iron acquisition is an important factor for pathogen-host interaction and also a significant elemen...
Self-excising integrative yeast plasmid vectors containing an intronated recombinase gene [0.03%]
含有内含子重组酶基因的自我消除型整合酵母质粒载体
Michael Agaphonov,Alexander Alexandrov
Michael Agaphonov
Site-specific recombinases are widely used for selectable marker recycling in molecular-genetic manipulations with eukaryotic cells. This usually involves the use of two genetic constructs, one of which possesses a selectable marker flanked...
The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae [0.03%]
转录激活因子Pog1调控细胞周期，其磷酸化形式通过泛素连接酶Dma2降解在酿酒酵母中的作用机制研究
Satoshi Oshiro,Hiroshi Takagi
Satoshi Oshiro
The POG1 gene in Saccharomyces cerevisiae is suggested to encode the transcriptional activator that promotes growth in the presence of a mating pheromone. We previously showed that the overexpression of POG1 conferred tolerance to high conc...