Integrating Yeast Biodiversity and Machine Learning for Predictive Metabolic Engineering [0.03%]
整合酵母多样性和机器学习进行预测代谢工程
Akaraphol Watcharawipas,Weerawat Runguphan,Peerapat Khamwachirapithak et al.
Akaraphol Watcharawipas et al.
Yeast biodiversity and machine learning (ML) are transforming the landscape of metabolic engineering. While Saccharomyces cerevisiae remains foundational to industrial biotechnology due to its genetic tractability and robust growth, it stru...
Adaptive laboratory evolution of Saccharomyces cerevisiae CEN.PK 113-7D to enhance ethanol tolerance [0.03%]
利用酿酒酵母自适应实验室进化提高耐乙醇性
Fatemeh Sheikhi,Mahsa Babaei,Khosrow Rostami et al.
Fatemeh Sheikhi et al.
Saccharomyces cerevisiae is a widely used yeast for industrial production of ethanol. However, elevated ethanol, temperature, and osmotic stress adversely affect fermentation efficiency. In this study, adaptive laboratory evolution for S. c...
Fungal β-1,3-Glucan Synthase: A Review of Structure, Mechanism, and Regulation [0.03%]
真菌β-1,3-葡聚糖合酶：结构、机制和调控综述
Xintong Huang,Muwu Chen,Zan Chen et al.
Xintong Huang et al.
Fungal β-1,3-glucan synthase (Fks) plays a central role in synthesizing β-1,3-glucan, the main structural polysaccharide of fungal cell walls, and serves as a key target for antifungal drugs such as echinocandins and ibrexafungerp. Recent...
The genetically encoded biosensor HyPer7 enables in-line monitoring of H2O2 accumulation dynamics in the methylotrophic yeast Komagataella phaffii [0.03%]
遗传编码的生物传感器HyPer7能够对甲基营养型酵母Komagataella phaffii中H2O2积累动力学进行在线监测
Victor Mendes Honorato,Jennifer Staudacher,Mikael Molin et al.
Victor Mendes Honorato et al.
In methylotrophic yeasts such as Komagataella phaffii (syn Pichia pastoris), the initial step of methanol utilization (MUT) by alcohol oxidase (Aox) generates H2O2 as a potentially toxic byproduct. Introduction of the ratiometric, genetical...
Genome-based exploration of volatile flavor diversity from food yeast species [0.03%]
基于基因组的食品酵母品种挥发性风味多样性探索
Su Jin Yoo,Da Eun Kim,Lintang Ignatius Satyawan et al.
Su Jin Yoo et al.
Yeast shares a longer than 10 000-year history with humans in food fermentation by producing various volatile flavor compounds that contribute to the final taste and aroma of foods. Yeast-associated volatile flavor compounds include esters,...
Yeast-secreted compounds with antifungal activity - screening, genetic parts, biosynthetic pathways and regulation [0.03%]
酵母分泌抗真菌化合物-筛选、功能元件、生物合成途径及调控
Alicia Maciá Valero,Min Lu,Sonja Billerbeck
Alicia Maciá Valero
Awareness is rising that antifungal resistance poses a threat to agriculture, food safety, biodiversity and human health. There is a limited number of antifungals available and resistance to all of them has been reported. The development of...
Engineering recombination machinery facilitates the construction of yeast cell factories [0.03%]
工程化基因重组系统促进酵母细胞工厂构建
Nan Jia,Yongjin J Zhou,Jiaoqi Gao
Nan Jia
Advances in genome editing have been promoted by programmable nucleases like CRISPR-Cas9, which triggers endogenous DNA repair mechanisms by inducing double-strand break (DSB). Cellular responses to DSBs are governed by competing repair pat...
Breeding of yeast strains with intracellular amino acid accumulation for value-added alcoholic beverages [0.03%]
用于增值酒精饮料的酵母菌株育种以积累体内氨基酸
Shota Isogai,Akira Nishimura,Hiroshi Takagi
Shota Isogai
The yeast Saccharomyces cerevisiae converts amino acids into volatile compounds with fruity and floral aromas during fermentation. These amino acid-derived aroma compounds play a critical role in defining the taste and flavor of alcoholic b...
Lighting up yeast: Overview of optogenetics in yeast and their applications to yeast biotechnology [0.03%]
点亮酵母：光遗传学在酵母中的概述及其在酵母生物技术中的应用
Jaewan Jang,José L Avalos
Jaewan Jang
Optogenetics is an empowering technology that uses light-responsive proteins to control biological processes. Because of its genetic tractability, abundance of genetic tools, and robust culturing conditions, Saccharomyces cerevisiae has ser...
Evolutionary Engineering and Molecular Characterization of an Antimycin A-Resistant Saccharomyces cerevisiae Strain: the Key Role of Pleiotropic Drug Resistance (PDR1) [0.03%]
进化工程和抗霉素A抗性酵母菌株的分子表征：多药耐药基因(PDR1)的关键作用
A Topaloğlu,C Holyavkin,Ö Esen et al.
A Topaloğlu et al.
Antimycin A, an antifungal agent that inhibits mitochondrial respiration, provides a useful model for studying resistance mechanisms. Antifungal resistance is an escalating clinical concern with limited treatment options available. To under...