Genomic tales of wine yeasts: from domestication to functional innovation [0.03%]
葡萄酒酵母的基因组故事：从驯化到功能创新
Carmen Becerra-Rodríguez,Hugo Devillers,Jean-Luc Legras et al.
Carmen Becerra-Rodríguez et al.
Over the last decades, the yeast Saccharomyces cerevisiae has emerged as a key model for studying microbial domestication, particularly in the context of winemaking. The recent surge of population genomic data, encompassing thousands of gen...
Simplifying multiplex genome engineering in Saccharomyces cerevisiae with intron-mediated Random Assembly and INtegration (RAIN) [0.03%]
利用介导的随机组装和整合技术简化酿酒酵母基因组工程化过程
Fergus S Harrison,Philip A Kelso,Alexander C Carpenter et al.
Fergus S Harrison et al.
Engineering of multistep enzymatic pathways often involves extensive optimization of heterologous gene expression levels and requires cloning of promoter and open reading frames (ORFs) to generate expression cassettes. We present work on a ...
The nicotinamide mononucleotide adenylyl transferase (NMNAT/Nma1) modulates phosphate-sensing (PHO) signaling independent of its NAD+ synthesis activity in Saccharomyces cerevisiae [0.03%]
烟酰胺单核苷酸腺嘌呤二核苷酸转移酶（NMNAT / Nma1）在酿酒酵母中通过独立于其NAD +合成活性的方式调节无机磷感应（PHO）信号传导
Yi-Ching Lee,Chi-Chun Huang,Matilda McDaniel et al.
Yi-Ching Lee et al.
Regulation of NAD+ metabolism is interconnected with multiple nutrient-sensing pathways and cellular processes. The phosphate (Pi)-sensing (PHO) signaling pathway contributes to NAD+ degradation, and PHO-responsive genes are reciprocally re...
G Brach,J Schacherer
G Brach
Over the past three decades, Saccharomyces cerevisiae has gone from being the first eukaryote to have its genome fully sequenced to one of the most studied biological systems. Advances in sequencing technologies, functional genomics, and po...
Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose [0.03%]
通过定向突变重构酿酒酵母马克斯克鲁维酵母Hgt家族糖转运蛋白以同时利用葡萄糖和木糖
Lorena Donzella,Carlos Belloch-Molina,John P Morrissey et al.
Lorena Donzella et al.
Efficient utilization of lignocellulosic hydrolysates in yeast-based biorefineries requires simultaneous consumption of glucose and xylose, which is often limited by preferential glucose uptake. In Kluyveromyces marxianus, we kinetically ch...
Adaptive laboratory evolution of maltose utilisation in wild Irish isolates of Saccharomyces eubayanus [0.03%]
麦芽糖利用的适应性实验室进化——以爱尔兰野生雪华葡萄酵母(Saccharomyces eubayanus)分离株为材料的研究
Sean Bergin,Jillian Scully,Conor Hession et al.
Sean Bergin et al.
The first European isolates of S. eubayanus were discovered in Ireland in 2022 and belong to the same clade as the S. eubayanus parent of the hybrid lager yeast S. pastorianus. Here, we report the isolation of ten additional Irish strains a...
Replicative age differentially predicts metabolic competence across industrial yeasts at single-cell resolution [0.03%]
replicative 年龄在工业酵母单细胞分辨率代谢能力上的不同预测能力
Marco Eigenfeld,Elisabeth Zach,Ann-Kathrin Bürkle et al.
Marco Eigenfeld et al.
In industrial yeast fermentations, population-level viability assays routinely report healthy cultures even as aged, metabolically compromised mother-cell subpopulations go undetected. Replicative age contributes to this hidden heterogeneit...
Life beyond 6,000 genes: From sequence to synthesis in a post-genomics era [0.03%]
后基因组时代的生命体：从序列到合成研究超六千个基因的生物体
Roy S K Walker,Edward Archer,Paige Erpf et al.
Roy S K Walker et al.
The publication of the Saccharomyces cerevisiae genome sequence thirty years ago marked a defining shift in modern biology, establishing yeast as the first fully sequenced eukaryotic model cell. Access to a complete reference genome has cat...
De novo Genome Assembly of a Native Saccharomyces cerevisiae Strain Isolated from Spontaneous Fermentation of Vineyard Must in the Atacama Desert (Chile) [0.03%]
从阿塔卡马沙漠葡萄汁自发发酵中分离的土著酿酒酵母菌株的从头基因组组装
Héctor Aguayo-Cumplido,Michelle Cifras,Marcelo Lanino et al.
Héctor Aguayo-Cumplido et al.
In this work, we investigated the genomic and phenotypic basis of stress tolerance in the native Saccharomyces cerevisiae strain M6, isolated from spontaneously fermenting must at a vineyard located in Atacama Desert, through a de novo hybr...
Transcriptional regulation: Efficient genetic engineering tools for non-conventional yeasts [0.03%]
转录调控：非传统酵母菌的高效基因工程技术
Shabana Haneef,Yongjin J Zhou,Fan Bai
Shabana Haneef
Non-conventional yeasts are recognized as valuable hosts for producing biofuels, pharmaceuticals, and other high-value chemicals, owing to their diverse physiological traits, ability to utilize various substrates, and greater tolerance to e...