Clinical Europium fluorescent based lectin assays for mucin O-glycomics [0.03%]
临床 europium 荧光基立信试验用于粘蛋白 o-糖链分析
Ali R Afshari,Marysol Bello-Gonzalez,Ida E Andersen et al.
Ali R Afshari et al.
In this chapter, we are presenting two assay formats for glycomic discovery and validation: Fluorescent Immune-Lectin Assay (FILA) and Fluorescent Lectin Assay (FLA). Both methods rely on conjugation of lectins to highly sensitive Europium ...
A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases [0.03%]
O-糖肽酶检测和定量分析的双色FRET测定法
Olivia Canil,Liam Mihalynuk,Nicole Thompson et al.
Olivia Canil et al.
Mammalian mucosal surfaces are coated with excreted mucus composed primarily of large, heavily O-glycosylated proteins known as mucins. Such extensive O-glycosylation often makes mucin recalcitrant to conventional proteases, making mucins c...
Petar Pajic,Omer Gokcumen
Petar Pajic
Mucins are heavily glycosylated proteins that form protective mucus barriers at host-environment interfaces. Mucin genes frequently contain exonic variable number tandem repeat (exVNTR) domains that encode peptides enriched in proline, thre...
Elisabeth E L Nyström,Brendan Dolan,George M H Birchenough et al.
Elisabeth E L Nyström et al.
The intestinal mucus layer is a highly hydrated hydrogel that forms a critical barrier separating the gut microbiota from the epithelial surface while permitting nutrient exchange and luminal transport. Despite its central role in intestina...
Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues [0.03%]
糖基-TRAPP：一种实时糖萼通透性测定法，用于评估活组织和固定组织中跨膜黏蛋白屏障功能
Fleur Claire van de Koolwijk,Thaher Pelaseyed
Fleur Claire van de Koolwijk
A glycocalyx permeability assay for in situ assessment of transmembrane mucin barrier function ABSTRACT: The small intestine lacks a dense, attached mucus layer and instead relies on an apical cell surface glycocalyx as an alternative barri...
Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ [0.03%]
定量化结肠黏液结构和功能动态的成像方法及其在健康和疾病中的应用
Megan Turluk,Benjamin Bigiremana,Darrek Kniffen et al.
Megan Turluk et al.
Mucins are highly glycosylated proteins, a subset of which form the structural basis of mucus barriers at mucosal surfaces. In the gastrointestinal tract, the secreted gel-forming mucin MUC2 is the principal component of the intestinal mucu...
Thishan Illankoon,Priyanka K Jaisinghani,Sumaira Z Hasnain
Thishan Illankoon
Mucins, the fundamental structural components of mucus, constitute a diverse family of complex glycoproteins. They are classified into two main categories: secreted mucins and surface-associated mucins, both of which serve critical roles in...
Shreyas Gupta,Guilherme Meira Lima,Chuanhao Peng et al.
Shreyas Gupta et al.
M13 phage makes it possible to produce DNA-encoded display of any molecules stable in water by prospective DNA-barcoding. For example, chemical conjugation of a glycan to an M13 virion that contains a prospectively introduced DNA barcode in...
Covalent crosslinking of mucins: From biomaterial design to tailored functions [0.03%]
黏蛋白的共价交联作用：从生物材料设计到特异性功能定制
Thomas Crouzier
Thomas Crouzier
Mucins are high-molecular-weight glycoproteins essential for the hydration, lubrication, and protective barrier functions of epithelial surfaces. Beyond these physical properties, their dense O-glycan brushes serve as potent multivalent lig...
Olga Sokolovskaya,Reuben Allen,Bradley Turner et al.
Olga Sokolovskaya et al.
Mucin glycoproteins are the major structural components of mucus, imparting its gel consistency. Not only do mucins form a protective barrier that traps debris and keeps pathogenic bacteria at a safe distance from host cells, but they displ...