Yuan Zhang,Ran Zhuang,Zhu-Wei Xu et al.
Yuan Zhang et al.
Disulfide oxidoreductase A (also known as disulfide bond formation protein A, or DsbA) is produced in Escherichia coli (E. coli) periplasm, which plays an important role in correct formulation of disulfide bonds during protein exportation i...
Structural and functional study of mouse antidigoxin monoclonal antibody against thermal variation [0.03%]
Soheila Kashanian,Sirous Ghobadi,Kobra Omidfar et al.
Soheila Kashanian et al.
Conformation, heat-induced conformational changes, and the stability of mouse monoclonal antibody against digoxin were analyzed by circular dichroism (CD), fluorescence, UV-Vis spectroscopy, and ELISA techniques. Since both biological funct...
Application of monoclonal antibodies in a rapid sandwich dot-enzyme linked immunosorbent assay for identification and antigen detection of Leptospira serovars [0.03%]
Rashmi Sharma,Urmil Tuteja,Rekha Khushiramani et al.
Rashmi Sharma et al.
Monoclonal antibodies (MAbs) were produced by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice that were immunized with live whole cells of the four most prevalent Leptospira serovars--namely, autumnalis, australis, grippotyphosa...
Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions [0.03%]
Sun Li Chong,Duen Gang Mou,Abdul Manaf Ali et al.
Sun Li Chong et al.
The effect of mild hypothermic (32 degrees C) conditions on cell growth, cell-cycle progress, and antibody production of hybridoma C2E7 cells was investigated in the present study. The growth of hybridoma cells was slower during the mild hy...
Comparative Study
Hybridoma (2005). 2008 Apr;27(2):107-11. DOI:10.1089/hyb.2007.0548 2008
Effect of osmolytes on the conformational stability of mouse monoclonal antidigoxin antibody in long-term storage [0.03%]
Soheila Kashanian,Maliheh Paknejad,Sirous Ghobadi et al.
Soheila Kashanian et al.
The natural selection of small organic molecules (osmolytes) that protect cellular proteins against denaturing stresses is characteristic of plants and animals that have adapted to environmental extremes. In this study the role of osmolytes...
Comparative Study
Hybridoma (2005). 2008 Apr;27(2):99-106. DOI:10.1089/hyb.2007.0554 2008
Andrea Rottach,Elisabeth Kremmer,Danny Nowak et al.
Andrea Rottach et al.
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This ...
Comparative Study
Hybridoma (2005). 2008 Apr;27(2):91-8. DOI:10.1089/hyb.2007.0555 2008
Brandon A Howard,Gregory D Sempowski,Richard M Scearce et al.
Brandon A Howard et al.
Human (h) CD7 is a 40 kDa single chain Ig superfamily molecule that is expressed on thymocytes, a major subunit of peripheral T cells, and most natural killer cells. Ligands for hCD7 include the epithelial cell-produced molecule, K-12, and ...
Comparative Study
Hybridoma (2005). 2008 Apr;27(2):81-9. DOI:10.1089/hyb.2007.0553 2008
Structural requirements for monoclonal antibody 2-1-L8 recognition of neisserial lipooligosaccharides [0.03%]
Ellen T O&#x;Connor,Karen V Swanson,Hui Cheng et al.
Ellen T O&#x;Connor et al.
Monoclonal antibodies (MAbs) that bind neisserial lipooligosaccharides (LOS) have been widely used in structural studies of these glycolipids. MAb 2-1-L8 binds LOS with a lactosyl a chain (Gal beta1-4 Glc beta1-4 [Glc-NAc alpha1-2 Hep2 alph...
Sharad P Adekar,Fetweh H Al-Saleem,M D Elias et al.
Sharad P Adekar et al.
Affinity-matured human antibodies have demonstrated efficacy as countermeasures for exposure to botulinum neurotoxin (BoNT), which is the cause of the disease botulism category A select bioterror agent. Little is known, however, about the p...
Expression of a splicing isoform of MAIR-V (CD300LF), an inhibitory immunoglobulin-like receptor on myeloid cells [0.03%]
髓系细胞抑制性免疫球蛋白样受体MAIR-V(CD300LF)的剪接异构体表达
Ismail Can,Satoko Tahara-Hanaoka,Akira Shibuya
Ismail Can
The myeloid-associated immunoglobulin-like receptor (MAIR) family consists of nine activating or inhibitory cell surface receptors preferentially expressed on myeloid cells, which are encoded by the genes in a small cluster on mouse chromos...