Ann-Sophie Kroell,Kira H Hoffmann,Nikolas A Motzkus et al.
Ann-Sophie Kroell et al.
Thermogenetics enables noninvasive spatiotemporal control over protein activity in living cells and tissues, yet its applications have largely been restricted to transcriptional regulation and membrane recruitment. Here, we present a genera...
NCBP1 stress signaling drives alternative S6K1 splicing inhibiting translation [0.03%]
NCBP1应激信号通过抑制S6K1的剪接来抑制蛋白质翻译
Dalu Chang,Mahdi Assari,Chananya Suwathep et al.
Dalu Chang et al.
Subcellular stress profoundly influences protein synthesis. However, both the nature of spatiotemporally restricted chemical cues and local protein responders to these cues remain elusive. Unlocking these mechanisms requires the ability to ...
Photosensitizer proximity labeling captures the lipid and protein interactomes [0.03%]
光敏剂邻近标记捕获脂质和蛋白质相互作用组
Andrew P Becker,Elijah Biletch,John Paul Kennelly et al.
Andrew P Becker et al.
The physical properties of cellular membranes are influenced by protein and lipid interactions. In situ proximity labeling interactomic methods are well suited to characterize these dynamic and often fleeting interactions. Yet, available me...
Olivia J Seidel,Itay Budin
Olivia J Seidel
Abasic CRISPR RNAs inherently harness fidelity of SpCas9 for genome editing [0.03%]
一种基本的CRISPR RNA可以利用SpCas9的基因组编辑保真度
Dowoon Gu,Geun-Woo D Kim,Mingyo Park et al.
Dowoon Gu et al.
CRISPR-Cas9, an RNA-guided immune system, functions specifically in bacteria while controlling autoimmunity. However, its application to genome editing often causes deleterious off-target cleavages. Here, by sequencing CRISPR RNAs (crRNAs),...
Ella Livnah,Ohad Suss,Adi Rogel et al.
Ella Livnah et al.
Molecules that facilitate protein-protein interactions are immensely impactful. However, such compounds typically rely on accessory proteins to function, such as E3 ligases for targeted degradation, which may restrict their scope or lead to...
Proteins feel the PINCH [0.03%]
蛋白质感受PINCH
Thomas Kodadek
Thomas Kodadek
Uncovering bacterial pseudaminylation with pan-specific antibody tools [0.03%]
具有广泛特异性的抗体工具揭示细菌假氨基化现象
Arthur H Tang,Niccolay Madiedo Soler,Kristian I Karlic et al.
Arthur H Tang et al.
Pseudaminic acids (Pse) are a family of carbohydrates found within bacterial lipopolysaccharides, capsular polysaccharides and glycoproteins that are critical for the virulence of human pathogens. However, a dearth of effective tools for de...
Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates [0.03%]
通过将OGT作用物和底物联系起来分析O-糖基化功能
Matthew E Griffin,John W Thompson,Yao Xiao et al.
Matthew E Griffin et al.
The post-translational modification (PTM) of proteins by O-linked β-N-acetyl-D-glucosamine (O-GlcNAcylation) is widely found across the proteome and regulates diverse cellular processes, from transcription and translation to signal transdu...
Jingyi Zhao,Alex Luebbers,Sofya Savransky et al.
Jingyi Zhao et al.
Gαs serves as the prototypical signal transducer for G-protein-coupled receptors (GPCRs) and is the heterotrimeric G protein most frequently mutated in cancer. The classical view of the plasma membrane as the only cellular location where G...